During 1997, samples of plants showing symptoms of a viral disease were collected from thirteen pepper growing areas. On some plants characteristic symptoms of Potato Virus Y (PVY) infection were observed. Pepper samples were tested by applying the direct immunoenzyme test (DAS ELISA). For the identification of the virus a PVY antiserum was used, i.e. the kit of Loewe Biochemica, GmbH Germany. Isolation of the virus was performed by the method of mechanical inoculation using 0.01 M phosphate buffer (pH-7) and 0.1% Na2SO3 from the infected pepper samples. Identification of the virus strains was performed on the basis of the reaction of differential pepper genotypes. The virus was identified according to pepper disease symptoms, DAS ELISA test, reaction of inoculated test plants, way of transmission and its biophysical constants. On the basis of the observed reaction of the differential pepper genotypes, two strains of this virus were identified.
Keywords:
DAS ELISA / host plants / virus strains
Source:
Proceedings of the Second Balkan Symposium on Vegetables and Potatoes, 2002, 579, 545-549
TY - CONF
AU - Mijatović, M
AU - Ivanović, M
AU - Obradović, Aleksa
AU - Zecević, Bogoljub
PY - 2002
UR - http://aspace.agrif.bg.ac.rs/handle/123456789/496
AB - During 1997, samples of plants showing symptoms of a viral disease were collected from thirteen pepper growing areas. On some plants characteristic symptoms of Potato Virus Y (PVY) infection were observed. Pepper samples were tested by applying the direct immunoenzyme test (DAS ELISA). For the identification of the virus a PVY antiserum was used, i.e. the kit of Loewe Biochemica, GmbH Germany. Isolation of the virus was performed by the method of mechanical inoculation using 0.01 M phosphate buffer (pH-7) and 0.1% Na2SO3 from the infected pepper samples. Identification of the virus strains was performed on the basis of the reaction of differential pepper genotypes. The virus was identified according to pepper disease symptoms, DAS ELISA test, reaction of inoculated test plants, way of transmission and its biophysical constants. On the basis of the observed reaction of the differential pepper genotypes, two strains of this virus were identified.
PB - Int Soc Horticultural Science, Leuven 1
C3 - Proceedings of the Second Balkan Symposium on Vegetables and Potatoes
T1 - Potato Virus Y (PVY) on pepper in Serbia
EP - 549
IS - 579
SP - 545
DO - 10.17660/ActaHortic.2002.579.96
ER -
@conference{
author = "Mijatović, M and Ivanović, M and Obradović, Aleksa and Zecević, Bogoljub",
year = "2002",
abstract = "During 1997, samples of plants showing symptoms of a viral disease were collected from thirteen pepper growing areas. On some plants characteristic symptoms of Potato Virus Y (PVY) infection were observed. Pepper samples were tested by applying the direct immunoenzyme test (DAS ELISA). For the identification of the virus a PVY antiserum was used, i.e. the kit of Loewe Biochemica, GmbH Germany. Isolation of the virus was performed by the method of mechanical inoculation using 0.01 M phosphate buffer (pH-7) and 0.1% Na2SO3 from the infected pepper samples. Identification of the virus strains was performed on the basis of the reaction of differential pepper genotypes. The virus was identified according to pepper disease symptoms, DAS ELISA test, reaction of inoculated test plants, way of transmission and its biophysical constants. On the basis of the observed reaction of the differential pepper genotypes, two strains of this virus were identified.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "Proceedings of the Second Balkan Symposium on Vegetables and Potatoes",
title = "Potato Virus Y (PVY) on pepper in Serbia",
pages = "549-545",
number = "579",
doi = "10.17660/ActaHortic.2002.579.96"
}
Mijatović, M., Ivanović, M., Obradović, A.,& Zecević, B.. (2002). Potato Virus Y (PVY) on pepper in Serbia. in Proceedings of the Second Balkan Symposium on Vegetables and Potatoes
Int Soc Horticultural Science, Leuven 1.(579), 545-549.
https://doi.org/10.17660/ActaHortic.2002.579.96
Mijatović M, Ivanović M, Obradović A, Zecević B. Potato Virus Y (PVY) on pepper in Serbia. in Proceedings of the Second Balkan Symposium on Vegetables and Potatoes. 2002;(579):545-549.
doi:10.17660/ActaHortic.2002.579.96 .
Mijatović, M, Ivanović, M, Obradović, Aleksa, Zecević, Bogoljub, "Potato Virus Y (PVY) on pepper in Serbia" in Proceedings of the Second Balkan Symposium on Vegetables and Potatoes, no. 579 (2002):545-549,
https://doi.org/10.17660/ActaHortic.2002.579.96 . .
