Приказ основних података о документу

dc.creatorSupramani, Sugenendran
dc.creatorJailani, Nurulhuda
dc.creatorRamarao, Kivaandra
dc.creatorZain, Nurul Amalina Mohd
dc.creatorKlaus, Anita
dc.creatorAhmad, Rahayu
dc.creatorWan-Mohtar, Wan Abd Al Qadr Imad
dc.date.accessioned2020-12-17T22:31:42Z
dc.date.available2020-12-17T22:31:42Z
dc.date.issued2019
dc.identifier.issn1878-8181
dc.identifier.urihttp://aspace.agrif.bg.ac.rs/handle/123456789/4959
dc.description.abstractThe pellet diameter and morphology of an underutilized European Ganoderma pfeifferi (EGP) mycelium was studied in a repeated-batch fermentation (RBF) for exopolysaccharide (EPS) production. In preliminary fermentation, growth screening was performed for initial pH, glucose concentration, and agitation speed. At 30 degrees C, the favourable conditions for EPS production (0.58 g/L) and EPS productivity (0.058 g/L day(-1)) were 15 g/L glucose, initial pH 6, and 100 rpm, while the highest dry cell weight (DCW; 3.63 g/L) was achieved at 9 g/L glucose, initial pH 4, and 120 rpm. Morphologically verified, large, compact pellets (diameter: 40 mu m lt d lt 40.67 mu m) were associated with high DCW while small dispersed pellets (diameter: 11.67 mu m lt d lt 14.67 mu m) were associated with high EPS production. In RBF, EGP mycelium exhibited the ability to self-immobilize and high stability for repeated use with increasing smooth pellet diameter from RBF1 (7.33 mu m lt d lt 7.67 mu m) to RBF4 (16.67 mu m lt d lt 18.33 mu m). The fermentation period was subsequently shortened from 48 (batch) to 24 days in four consecutive cycles of RBF, and the productivity of EPS increased from 0.053 g/L day(-1) to 0.108 g/L day(-1). In an FTIR analysis, crude EPS of EGP showed the presence of beta-glucan characteristics at 1075 cm(-1) and 891 cm(-1) wavelengths, similar with European G. lucidum BCCM 31549. In addition, H-1 NMR showed similar beta-glycosidic linkages when compared with laminarin standard. The proposed strategy demonstrated that G. pfeifferi pellet morphology can withstand extended fermentation cycles for efficient EPS production.en
dc.publisherElsevier, Amsterdam
dc.relationMinistry of Education MalaysiaMinistry of Education, Malaysia [FP066-2018A, RU003I-2017]
dc.rightsrestrictedAccess
dc.sourceBiocatalysis and Agricultural Biotechnology
dc.subjectGanoderma pfeifferien
dc.subjectPellet morphologyen
dc.subjectPellet diameteren
dc.subjectExopolysaccharideen
dc.subjectbeta-glucanen
dc.subjectRepeated-batch fermentationen
dc.titlePellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide productionen
dc.typearticle
dc.rights.licenseARR
dc.citation.other19: -
dc.citation.volume19
dc.identifier.doi10.1016/j.bcab.2019.101118
dc.identifier.scopus2-s2.0-85064011008
dc.identifier.wos000477644800025
dc.type.versionpublishedVersion


Документи

Thumbnail

Овај документ се појављује у следећим колекцијама

Приказ основних података о документу