In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum
Само за регистроване кориснике
2019
Аутори
Vasić, TanjaVojinović, Uroš
Zujović, Suzana
Krnjaja, Vesna
Zivković, Sanja
Marković, Jordan
Stević, Milan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Sensitivity of 24 isolates of Colletotrichum destructivum O'Gara, collected from alfalfa plants in Serbia, to eight selected fungicides, was investigated in this study. Molecular identification and pathogenicity test of isolates tested were also performed. Fungicide sensitivity was evaluated in vitro, using mycelial growth assay method. All isolates exhibited significant pathogenicity, causing necrosis at the alfalfa seedling root tips two days after inoculation. Using the primer pair GSF1-SR1 and by comparing the amplified fragments of the tested isolates with the marker (M), the presence of the amplicon of the expected size of about 900 bp was determined for all isolates. The isolates tested in this study showed different sensitivity towards fungicides in vitro. Mycelial growth was highly inhibited by QoI (quinone outside inhibitors) fungicide pyraclostrobin (mean EC50=0.39 mu g mL(-1)) and by DMI (demethylation-inhibiting) fungicide tebuconazole (mean EC50=0.61 mu g mL(-1)), followe...d by azoxystrobin (mean EC50=2.83 mu g mL(-1)) and flutriafol (mean EC50=2.11 mu g mL(-1)). Multi-site fungicide chlorothalonil and MBC (methyl benzimidazole carbamate) fungicide thiophanate-methyl evinced moderate inhibition with mean EC50=35.31 and 62.83 mu g mL(-1), respectively. Thirteen isolates were sensitive to SDHI (succinate dehydrogenase inhibitors) fungicide boscalid and fluxapyroxad, (mean EC50=0.49 and 0.19 mu g mL(-1), respectively), while the rest of isolates were highly resistant.
Кључне речи:
Fungicide sensitivity / DMI / QoI / SDHI / molecular characterization / pathogenicityИзвор:
Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was, 2019, 54, 12, 964-971Издавач:
- Taylor & Francis Inc, Philadelphia
Финансирање / пројекти:
- Побољшање генетичког потенцијала и технологија производње крмног биља у функцији одрживог развоја сточарства (RS-MESTD-Technological Development (TD or TR)-31057)
DOI: 10.1080/03601234.2019.1653735
ISSN: 0360-1234
PubMed: 31429367
WoS: 000482289700001
Scopus: 2-s2.0-85070982025
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Vasić, Tanja AU - Vojinović, Uroš AU - Zujović, Suzana AU - Krnjaja, Vesna AU - Zivković, Sanja AU - Marković, Jordan AU - Stević, Milan PY - 2019 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4954 AB - Sensitivity of 24 isolates of Colletotrichum destructivum O'Gara, collected from alfalfa plants in Serbia, to eight selected fungicides, was investigated in this study. Molecular identification and pathogenicity test of isolates tested were also performed. Fungicide sensitivity was evaluated in vitro, using mycelial growth assay method. All isolates exhibited significant pathogenicity, causing necrosis at the alfalfa seedling root tips two days after inoculation. Using the primer pair GSF1-SR1 and by comparing the amplified fragments of the tested isolates with the marker (M), the presence of the amplicon of the expected size of about 900 bp was determined for all isolates. The isolates tested in this study showed different sensitivity towards fungicides in vitro. Mycelial growth was highly inhibited by QoI (quinone outside inhibitors) fungicide pyraclostrobin (mean EC50=0.39 mu g mL(-1)) and by DMI (demethylation-inhibiting) fungicide tebuconazole (mean EC50=0.61 mu g mL(-1)), followed by azoxystrobin (mean EC50=2.83 mu g mL(-1)) and flutriafol (mean EC50=2.11 mu g mL(-1)). Multi-site fungicide chlorothalonil and MBC (methyl benzimidazole carbamate) fungicide thiophanate-methyl evinced moderate inhibition with mean EC50=35.31 and 62.83 mu g mL(-1), respectively. Thirteen isolates were sensitive to SDHI (succinate dehydrogenase inhibitors) fungicide boscalid and fluxapyroxad, (mean EC50=0.49 and 0.19 mu g mL(-1), respectively), while the rest of isolates were highly resistant. PB - Taylor & Francis Inc, Philadelphia T2 - Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was T1 - In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum EP - 971 IS - 12 SP - 964 VL - 54 DO - 10.1080/03601234.2019.1653735 ER -
@article{ author = "Vasić, Tanja and Vojinović, Uroš and Zujović, Suzana and Krnjaja, Vesna and Zivković, Sanja and Marković, Jordan and Stević, Milan", year = "2019", abstract = "Sensitivity of 24 isolates of Colletotrichum destructivum O'Gara, collected from alfalfa plants in Serbia, to eight selected fungicides, was investigated in this study. Molecular identification and pathogenicity test of isolates tested were also performed. Fungicide sensitivity was evaluated in vitro, using mycelial growth assay method. All isolates exhibited significant pathogenicity, causing necrosis at the alfalfa seedling root tips two days after inoculation. Using the primer pair GSF1-SR1 and by comparing the amplified fragments of the tested isolates with the marker (M), the presence of the amplicon of the expected size of about 900 bp was determined for all isolates. The isolates tested in this study showed different sensitivity towards fungicides in vitro. Mycelial growth was highly inhibited by QoI (quinone outside inhibitors) fungicide pyraclostrobin (mean EC50=0.39 mu g mL(-1)) and by DMI (demethylation-inhibiting) fungicide tebuconazole (mean EC50=0.61 mu g mL(-1)), followed by azoxystrobin (mean EC50=2.83 mu g mL(-1)) and flutriafol (mean EC50=2.11 mu g mL(-1)). Multi-site fungicide chlorothalonil and MBC (methyl benzimidazole carbamate) fungicide thiophanate-methyl evinced moderate inhibition with mean EC50=35.31 and 62.83 mu g mL(-1), respectively. Thirteen isolates were sensitive to SDHI (succinate dehydrogenase inhibitors) fungicide boscalid and fluxapyroxad, (mean EC50=0.49 and 0.19 mu g mL(-1), respectively), while the rest of isolates were highly resistant.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was", title = "In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum", pages = "971-964", number = "12", volume = "54", doi = "10.1080/03601234.2019.1653735" }
Vasić, T., Vojinović, U., Zujović, S., Krnjaja, V., Zivković, S., Marković, J.,& Stević, M.. (2019). In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum. in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was Taylor & Francis Inc, Philadelphia., 54(12), 964-971. https://doi.org/10.1080/03601234.2019.1653735
Vasić T, Vojinović U, Zujović S, Krnjaja V, Zivković S, Marković J, Stević M. In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum. in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was. 2019;54(12):964-971. doi:10.1080/03601234.2019.1653735 .
Vasić, Tanja, Vojinović, Uroš, Zujović, Suzana, Krnjaja, Vesna, Zivković, Sanja, Marković, Jordan, Stević, Milan, "In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum" in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was, 54, no. 12 (2019):964-971, https://doi.org/10.1080/03601234.2019.1653735 . .