Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil

2019
Authors
Ilić, Dora S.Dimkić, Ivica

Waisi, Hadi K.
Gkorezis, Panagiotis M.
Hamidović, Saud
Raičević, Vera

Lalević, Blažo

Article (Published version)
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Hexavalent chromium, Cr(VI), one of the major pollutants from industrial facilities, is very toxic and harmful for human health and environmental quaky. Due to the lack of conventional methods, bioremediation was recommended as an environmentally friendly and effective technique. The aim of this paper was the isolation, identification and selection of the microorganisms which are capable of Cr(VI) reduction in vitro. Heavy metal concentration, detected in four soil samples, within and around the former bicycle factory Wog" (Republic . of Slovenia), was measured using the ICP-OES method. Bacteria were isolated and tested for chromium tolerance using LB agar supplemented with various Cr(VI) concentrations, whilst Cr(VI) reduction and bacterial growth was determined using the LB liquid medium. From 53 bacterial isolates, five of them showed a tolerance of 1000 mg/L of Cr(VI). Those five isolates showed the capability of growth under various Cr(VI) concentrations (50-1000 mg/L). Initial Cr...(VI) concentrations ranging from 50 to 100 mg/L were completely reduced by four bacterial isolates, whilst 500 to 1000 mg/L by Bacillus safensis 342-9. Using 16S rDNA and tuf gene sequence analyses, isolates 270-9R and 342-9 were identified as Bacillus safensis, isolates 351-9 and 270-9C as Bacillus subtilis subsp. subtilis, and 212-9 as Bacillus thuringiensis. These results indicated that these bacteria may be promising tools for remediation of metal-polluted sites.
Keywords:
Bacillus spp. / bioremediation / heavy metal pollution / hexa-valent chromium reduction / indigenous bacteriaSource:
Chemical Industry and Chemical Engineering Quarterly / CICEQ, 2019, 25, 3, 247-258Publisher:
- Savez hemijskih inženjera, Beograd
Funding / projects:
- Biodiversity as potential in ecoremediation technologies of degraded ecosystems (RS-31080)
- Molecular characterization of bacteria from genera Bacillus and Pseudomonas as potential agents for biological control (RS-173026)
DOI: 10.2298/CICEQ180607003I
ISSN: 1451-9372
WoS: 000489133800005
Scopus: 2-s2.0-85075595339
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Poljoprivredni fakultetTY - JOUR AU - Ilić, Dora S. AU - Dimkić, Ivica AU - Waisi, Hadi K. AU - Gkorezis, Panagiotis M. AU - Hamidović, Saud AU - Raičević, Vera AU - Lalević, Blažo PY - 2019 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4922 AB - Hexavalent chromium, Cr(VI), one of the major pollutants from industrial facilities, is very toxic and harmful for human health and environmental quaky. Due to the lack of conventional methods, bioremediation was recommended as an environmentally friendly and effective technique. The aim of this paper was the isolation, identification and selection of the microorganisms which are capable of Cr(VI) reduction in vitro. Heavy metal concentration, detected in four soil samples, within and around the former bicycle factory Wog" (Republic . of Slovenia), was measured using the ICP-OES method. Bacteria were isolated and tested for chromium tolerance using LB agar supplemented with various Cr(VI) concentrations, whilst Cr(VI) reduction and bacterial growth was determined using the LB liquid medium. From 53 bacterial isolates, five of them showed a tolerance of 1000 mg/L of Cr(VI). Those five isolates showed the capability of growth under various Cr(VI) concentrations (50-1000 mg/L). Initial Cr(VI) concentrations ranging from 50 to 100 mg/L were completely reduced by four bacterial isolates, whilst 500 to 1000 mg/L by Bacillus safensis 342-9. Using 16S rDNA and tuf gene sequence analyses, isolates 270-9R and 342-9 were identified as Bacillus safensis, isolates 351-9 and 270-9C as Bacillus subtilis subsp. subtilis, and 212-9 as Bacillus thuringiensis. These results indicated that these bacteria may be promising tools for remediation of metal-polluted sites. PB - Savez hemijskih inženjera, Beograd T2 - Chemical Industry and Chemical Engineering Quarterly / CICEQ T1 - Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil EP - 258 IS - 3 SP - 247 VL - 25 DO - 10.2298/CICEQ180607003I ER -
@article{ author = "Ilić, Dora S. and Dimkić, Ivica and Waisi, Hadi K. and Gkorezis, Panagiotis M. and Hamidović, Saud and Raičević, Vera and Lalević, Blažo", year = "2019", abstract = "Hexavalent chromium, Cr(VI), one of the major pollutants from industrial facilities, is very toxic and harmful for human health and environmental quaky. Due to the lack of conventional methods, bioremediation was recommended as an environmentally friendly and effective technique. The aim of this paper was the isolation, identification and selection of the microorganisms which are capable of Cr(VI) reduction in vitro. Heavy metal concentration, detected in four soil samples, within and around the former bicycle factory Wog" (Republic . of Slovenia), was measured using the ICP-OES method. Bacteria were isolated and tested for chromium tolerance using LB agar supplemented with various Cr(VI) concentrations, whilst Cr(VI) reduction and bacterial growth was determined using the LB liquid medium. From 53 bacterial isolates, five of them showed a tolerance of 1000 mg/L of Cr(VI). Those five isolates showed the capability of growth under various Cr(VI) concentrations (50-1000 mg/L). Initial Cr(VI) concentrations ranging from 50 to 100 mg/L were completely reduced by four bacterial isolates, whilst 500 to 1000 mg/L by Bacillus safensis 342-9. Using 16S rDNA and tuf gene sequence analyses, isolates 270-9R and 342-9 were identified as Bacillus safensis, isolates 351-9 and 270-9C as Bacillus subtilis subsp. subtilis, and 212-9 as Bacillus thuringiensis. These results indicated that these bacteria may be promising tools for remediation of metal-polluted sites.", publisher = "Savez hemijskih inženjera, Beograd", journal = "Chemical Industry and Chemical Engineering Quarterly / CICEQ", title = "Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil", pages = "258-247", number = "3", volume = "25", doi = "10.2298/CICEQ180607003I" }
Ilić, D. S., Dimkić, I., Waisi, H. K., Gkorezis, P. M., Hamidović, S., Raičević, V.,& Lalević, B.. (2019). Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil. in Chemical Industry and Chemical Engineering Quarterly / CICEQ Savez hemijskih inženjera, Beograd., 25(3), 247-258. https://doi.org/10.2298/CICEQ180607003I
Ilić DS, Dimkić I, Waisi HK, Gkorezis PM, Hamidović S, Raičević V, Lalević B. Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil. in Chemical Industry and Chemical Engineering Quarterly / CICEQ. 2019;25(3):247-258. doi:10.2298/CICEQ180607003I .
Ilić, Dora S., Dimkić, Ivica, Waisi, Hadi K., Gkorezis, Panagiotis M., Hamidović, Saud, Raičević, Vera, Lalević, Blažo, "Reduction of hexavalent chromium by bacillusspp. Isolated from heavy metal-polluted soil" in Chemical Industry and Chemical Engineering Quarterly / CICEQ, 25, no. 3 (2019):247-258, https://doi.org/10.2298/CICEQ180607003I . .