Beer brewing with immobilized yeast cells - Current experiance and recent results
Apstrakt
Immobilization of yeast cells offers many potential advantages in beer brewing and during the recent years, it has gained increasing interest in brewing industry. Over free cell systems, such as higher cell densities and loads, it enables much higher fermentation rates and remarkable shortening of the brewing process. This paper outlines several favourable bioreactor designs for primary beer fermentation and results obtained in internal loop gas-lift bioreactor system, introduced in this field by our research group. Several extrusion techniques for cell immobilization were investigated for production of alginate beads. Application of extrusion under gravity force, extrusion under coaxial air-jet and electrostatic droplet generation resulted in beads with diameters which ranged from 0.3 to 2 mm. These beads were successfully used in repeated batch and continuous beer fermentation in an internal loop gas-lift bioreactor for up to 3 months. Alginate was shown to be a suitable carrier for ...yeast cells. During a period of 3 months immobilized yeast cells have shown unchangeable activity and viability. Fermentation time was reduced below 1 day and final beers had desired sensory and analytical profiles.
Ključne reči:
beer / immobilized yeast / internal loop gas-lift bioreactor / alginate beads / extrusionIzvor:
Proceedings of the 4th Croatian Congress of Food Technologist, Biotechnologists, 2002, 296-302Izdavač:
- Univ Zagreb, Zagreb
Institucija/grupa
Poljoprivredni fakultetTY - CONF AU - Leskošek-Čukalović, Ida AU - Nedović, Viktor PY - 2002 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/480 AB - Immobilization of yeast cells offers many potential advantages in beer brewing and during the recent years, it has gained increasing interest in brewing industry. Over free cell systems, such as higher cell densities and loads, it enables much higher fermentation rates and remarkable shortening of the brewing process. This paper outlines several favourable bioreactor designs for primary beer fermentation and results obtained in internal loop gas-lift bioreactor system, introduced in this field by our research group. Several extrusion techniques for cell immobilization were investigated for production of alginate beads. Application of extrusion under gravity force, extrusion under coaxial air-jet and electrostatic droplet generation resulted in beads with diameters which ranged from 0.3 to 2 mm. These beads were successfully used in repeated batch and continuous beer fermentation in an internal loop gas-lift bioreactor for up to 3 months. Alginate was shown to be a suitable carrier for yeast cells. During a period of 3 months immobilized yeast cells have shown unchangeable activity and viability. Fermentation time was reduced below 1 day and final beers had desired sensory and analytical profiles. PB - Univ Zagreb, Zagreb C3 - Proceedings of the 4th Croatian Congress of Food Technologist, Biotechnologists T1 - Beer brewing with immobilized yeast cells - Current experiance and recent results EP - 302 SP - 296 UR - https://hdl.handle.net/21.15107/rcub_agrospace_480 ER -
@conference{ author = "Leskošek-Čukalović, Ida and Nedović, Viktor", year = "2002", abstract = "Immobilization of yeast cells offers many potential advantages in beer brewing and during the recent years, it has gained increasing interest in brewing industry. Over free cell systems, such as higher cell densities and loads, it enables much higher fermentation rates and remarkable shortening of the brewing process. This paper outlines several favourable bioreactor designs for primary beer fermentation and results obtained in internal loop gas-lift bioreactor system, introduced in this field by our research group. Several extrusion techniques for cell immobilization were investigated for production of alginate beads. Application of extrusion under gravity force, extrusion under coaxial air-jet and electrostatic droplet generation resulted in beads with diameters which ranged from 0.3 to 2 mm. These beads were successfully used in repeated batch and continuous beer fermentation in an internal loop gas-lift bioreactor for up to 3 months. Alginate was shown to be a suitable carrier for yeast cells. During a period of 3 months immobilized yeast cells have shown unchangeable activity and viability. Fermentation time was reduced below 1 day and final beers had desired sensory and analytical profiles.", publisher = "Univ Zagreb, Zagreb", journal = "Proceedings of the 4th Croatian Congress of Food Technologist, Biotechnologists", title = "Beer brewing with immobilized yeast cells - Current experiance and recent results", pages = "302-296", url = "https://hdl.handle.net/21.15107/rcub_agrospace_480" }
Leskošek-Čukalović, I.,& Nedović, V.. (2002). Beer brewing with immobilized yeast cells - Current experiance and recent results. in Proceedings of the 4th Croatian Congress of Food Technologist, Biotechnologists Univ Zagreb, Zagreb., 296-302. https://hdl.handle.net/21.15107/rcub_agrospace_480
Leskošek-Čukalović I, Nedović V. Beer brewing with immobilized yeast cells - Current experiance and recent results. in Proceedings of the 4th Croatian Congress of Food Technologist, Biotechnologists. 2002;:296-302. https://hdl.handle.net/21.15107/rcub_agrospace_480 .
Leskošek-Čukalović, Ida, Nedović, Viktor, "Beer brewing with immobilized yeast cells - Current experiance and recent results" in Proceedings of the 4th Croatian Congress of Food Technologist, Biotechnologists (2002):296-302, https://hdl.handle.net/21.15107/rcub_agrospace_480 .