Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia
2018
Autori
Krnjaja, VesnaStanković, Slavica
Obradović, Ana
Petrović, Tanja
Mandić, Violeta
Bijelić, Zorica
Božić, Manja
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 mu g kg(-1)) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequ...ent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.
Ključne reči:
winter wheat / Fusarium head blight / Fusarium graminearum / trichothecene genotypesIzvor:
Toxins, 2018, 10, 11Izdavač:
- MDPI, BASEL
Finansiranje / projekti:
- Redukcija toksigenih gljiva roda Fusarium i njihovih mikotoksina u proizvodnji zdravstveno bezbedne hrane na bazi žita (RS-MESTD-Technological Development (TD or TR)-31023)
- Primena novih biotehnoloških rešenja gajenja goveda, ovaca i koza u cilju dobijanja biološki vredne i zdravstveno bezbedne hrane (RS-MESTD-Technological Development (TD or TR)-31053)
- Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.3390/toxins10110460
ISSN: 2072-6651
PubMed: 30413020
WoS: 000451315900039
Scopus: 2-s2.0-85056429593
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Krnjaja, Vesna AU - Stanković, Slavica AU - Obradović, Ana AU - Petrović, Tanja AU - Mandić, Violeta AU - Bijelić, Zorica AU - Božić, Manja PY - 2018 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4716 AB - Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 mu g kg(-1)) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis. PB - MDPI, BASEL T2 - Toxins T1 - Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia IS - 11 VL - 10 DO - 10.3390/toxins10110460 ER -
@article{ author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Mandić, Violeta and Bijelić, Zorica and Božić, Manja", year = "2018", abstract = "Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 mu g kg(-1)) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.", publisher = "MDPI, BASEL", journal = "Toxins", title = "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia", number = "11", volume = "10", doi = "10.3390/toxins10110460" }
Krnjaja, V., Stanković, S., Obradović, A., Petrović, T., Mandić, V., Bijelić, Z.,& Božić, M.. (2018). Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia. in Toxins MDPI, BASEL., 10(11). https://doi.org/10.3390/toxins10110460
Krnjaja V, Stanković S, Obradović A, Petrović T, Mandić V, Bijelić Z, Božić M. Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia. in Toxins. 2018;10(11). doi:10.3390/toxins10110460 .
Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Petrović, Tanja, Mandić, Violeta, Bijelić, Zorica, Božić, Manja, "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia" in Toxins, 10, no. 11 (2018), https://doi.org/10.3390/toxins10110460 . .