Listeria monocytogenes - Danger for health safety vegetable production
Samo za registrovane korisnike
2018
Autori
Kljujev, IgorRaičević, Vera
Jovičić-Petrović, Jelena
Vujović, Bojana
Mirković, Milica
Rothballer, Michael
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The microbiologically contaminated vegetables represent a risk for consumers, especially vegetables without thermal processing. It is known that human pathogen bacteria, such as Listeria monocytogenes, could exist on fresh vegetables. The fresh vegetables could become Listeria-contaminated if they come in touch with contaminated soil, manure, irrigation water. The aim of this work was to investigate the presence of Listeria spp. and L. monocytogenes in different kind of vegetables grown in field and greenhouse condition as well as surface and endophytic colonization plant roots of different vegetables species by L. monocytogenes in laboratory conditions. The detection of Listeria spp. and L. monocytogenes in vegetable samples was done using ISO and PCR methods. The investigation of colonization vegetable roots and detection Listeria-cells inside plant root tissue was done using Fluorescence in situ hybridization (FISH) method in combination with confocal laser scanning microscopy (CLSM...). The results showed that 25.58% vegetable samples were positive for Listeria spp. and only one sample (carrot) was positive for L. monocytogenes out of 43 samples in total collected from field and greenhouse. The strain L. monocytogenes EGD-E surface and endophytic colonized carrot root in highest degree while strain L. monocytogenes SV4B was the most represented at leafy vegetable plants, such at lettuce (1.68 x 10(6) cells/mm(3) absolutely dry root) and spinach (1.39 x 10(6) cells/mm(3) absolutely dry root) root surface. The cells of L. monocytogenes SV4B were visible as single cells in interior tissue of plant roots (celery and sweet corn roots) as well as in the interior of the plant root cell at sweet corn root. The cells of L. monocytogenes EGD-E bind to the surface of the plant root and they were less commonly found out on root hair. In the inner layers of the root, those bacterial cells were inhabited intercellular spaces mainly as single cells very close to the larval vessels of root. Our results suggest that L. monocytogenes is very good endophytic colonizer of vegetable plant roots.
Ključne reči:
Contamination of vegetables / Listeria monocytogenes / Irrigation water / FISH / CLSMIzvor:
Microbial Pathogenesis, 2018, 120, 23-31Izdavač:
- Academic Press Ltd- Elsevier Science Ltd, London
Finansiranje / projekti:
DOI: 10.1016/j.micpath.2018.04.034
ISSN: 0882-4010
PubMed: 29684542
WoS: 000452578300004
Scopus: 2-s2.0-85046160977
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Kljujev, Igor AU - Raičević, Vera AU - Jovičić-Petrović, Jelena AU - Vujović, Bojana AU - Mirković, Milica AU - Rothballer, Michael PY - 2018 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4692 AB - The microbiologically contaminated vegetables represent a risk for consumers, especially vegetables without thermal processing. It is known that human pathogen bacteria, such as Listeria monocytogenes, could exist on fresh vegetables. The fresh vegetables could become Listeria-contaminated if they come in touch with contaminated soil, manure, irrigation water. The aim of this work was to investigate the presence of Listeria spp. and L. monocytogenes in different kind of vegetables grown in field and greenhouse condition as well as surface and endophytic colonization plant roots of different vegetables species by L. monocytogenes in laboratory conditions. The detection of Listeria spp. and L. monocytogenes in vegetable samples was done using ISO and PCR methods. The investigation of colonization vegetable roots and detection Listeria-cells inside plant root tissue was done using Fluorescence in situ hybridization (FISH) method in combination with confocal laser scanning microscopy (CLSM). The results showed that 25.58% vegetable samples were positive for Listeria spp. and only one sample (carrot) was positive for L. monocytogenes out of 43 samples in total collected from field and greenhouse. The strain L. monocytogenes EGD-E surface and endophytic colonized carrot root in highest degree while strain L. monocytogenes SV4B was the most represented at leafy vegetable plants, such at lettuce (1.68 x 10(6) cells/mm(3) absolutely dry root) and spinach (1.39 x 10(6) cells/mm(3) absolutely dry root) root surface. The cells of L. monocytogenes SV4B were visible as single cells in interior tissue of plant roots (celery and sweet corn roots) as well as in the interior of the plant root cell at sweet corn root. The cells of L. monocytogenes EGD-E bind to the surface of the plant root and they were less commonly found out on root hair. In the inner layers of the root, those bacterial cells were inhabited intercellular spaces mainly as single cells very close to the larval vessels of root. Our results suggest that L. monocytogenes is very good endophytic colonizer of vegetable plant roots. PB - Academic Press Ltd- Elsevier Science Ltd, London T2 - Microbial Pathogenesis T1 - Listeria monocytogenes - Danger for health safety vegetable production EP - 31 SP - 23 VL - 120 DO - 10.1016/j.micpath.2018.04.034 ER -
@article{ author = "Kljujev, Igor and Raičević, Vera and Jovičić-Petrović, Jelena and Vujović, Bojana and Mirković, Milica and Rothballer, Michael", year = "2018", abstract = "The microbiologically contaminated vegetables represent a risk for consumers, especially vegetables without thermal processing. It is known that human pathogen bacteria, such as Listeria monocytogenes, could exist on fresh vegetables. The fresh vegetables could become Listeria-contaminated if they come in touch with contaminated soil, manure, irrigation water. The aim of this work was to investigate the presence of Listeria spp. and L. monocytogenes in different kind of vegetables grown in field and greenhouse condition as well as surface and endophytic colonization plant roots of different vegetables species by L. monocytogenes in laboratory conditions. The detection of Listeria spp. and L. monocytogenes in vegetable samples was done using ISO and PCR methods. The investigation of colonization vegetable roots and detection Listeria-cells inside plant root tissue was done using Fluorescence in situ hybridization (FISH) method in combination with confocal laser scanning microscopy (CLSM). The results showed that 25.58% vegetable samples were positive for Listeria spp. and only one sample (carrot) was positive for L. monocytogenes out of 43 samples in total collected from field and greenhouse. The strain L. monocytogenes EGD-E surface and endophytic colonized carrot root in highest degree while strain L. monocytogenes SV4B was the most represented at leafy vegetable plants, such at lettuce (1.68 x 10(6) cells/mm(3) absolutely dry root) and spinach (1.39 x 10(6) cells/mm(3) absolutely dry root) root surface. The cells of L. monocytogenes SV4B were visible as single cells in interior tissue of plant roots (celery and sweet corn roots) as well as in the interior of the plant root cell at sweet corn root. The cells of L. monocytogenes EGD-E bind to the surface of the plant root and they were less commonly found out on root hair. In the inner layers of the root, those bacterial cells were inhabited intercellular spaces mainly as single cells very close to the larval vessels of root. Our results suggest that L. monocytogenes is very good endophytic colonizer of vegetable plant roots.", publisher = "Academic Press Ltd- Elsevier Science Ltd, London", journal = "Microbial Pathogenesis", title = "Listeria monocytogenes - Danger for health safety vegetable production", pages = "31-23", volume = "120", doi = "10.1016/j.micpath.2018.04.034" }
Kljujev, I., Raičević, V., Jovičić-Petrović, J., Vujović, B., Mirković, M.,& Rothballer, M.. (2018). Listeria monocytogenes - Danger for health safety vegetable production. in Microbial Pathogenesis Academic Press Ltd- Elsevier Science Ltd, London., 120, 23-31. https://doi.org/10.1016/j.micpath.2018.04.034
Kljujev I, Raičević V, Jovičić-Petrović J, Vujović B, Mirković M, Rothballer M. Listeria monocytogenes - Danger for health safety vegetable production. in Microbial Pathogenesis. 2018;120:23-31. doi:10.1016/j.micpath.2018.04.034 .
Kljujev, Igor, Raičević, Vera, Jovičić-Petrović, Jelena, Vujović, Bojana, Mirković, Milica, Rothballer, Michael, "Listeria monocytogenes - Danger for health safety vegetable production" in Microbial Pathogenesis, 120 (2018):23-31, https://doi.org/10.1016/j.micpath.2018.04.034 . .