Fluorescence microscopy as a tool for determining self-incompatibility in apricot cultivars
Abstract
Fluorescence microscopy is a relatively rapid and reliable method to determine self-incompatibility in fruit-tree species. It is based on observation of pollen-tube growth in the pistils. Pollen tubes stained with fluorochromes show fluorescence when exposed to ultraviolet light. Testing of the self-compatibility trait was carried out in 123 apricot cultivars using fluorescence microscopy. In self-compatible cultivars, in the majority of pistils (60-100%), the pollen tubes reached the ovary. In contrast, in self-incompatible cultivars, pollen tubes growth ceased in the style, with plugs forming at their tips. In these cultivars, pollen tubes rarely (0-30%) reached the base of the style. Although apricot cultivars of the European eco-geographical group are traditionally considered self-compatible, we identified many self-incompatible cultivars, especially among those originating from new North American and West European breeding programs. About half ( 62) of the studied cultivars were s...elf-incompatible. Given that self-incompatibility occurs frequently among new apricot cultivars, special care should be taken when considering cultivar composition in new orchard plantings.
Keywords:
Prunus armeniaca / self-incompatibility / pollen tube growth / fluorescence microscopySource:
XVI International Symposium on Apricot Breeding and Culture, 2018, 1214, 7-13Publisher:
- Int Soc Horticultural Science, Leuven 1
Funding / projects:
- The application of new genotypes and technological innovations for the purpose of improvement of fruit-growing and viticultural production (RS-31063)
- Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (EU-316004)
DOI: 10.17660/ActaHortic.2018.1214.2
ISSN: 0567-7572
WoS: 000467800200002
Scopus: 2-s2.0-85056820689
Collections
Institution/Community
Poljoprivredni fakultetTY - CONF AU - Milatović, Dragan AU - Nikolić, D. AU - Radović, Aleksandar AU - Krska, B. PY - 2018 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4626 AB - Fluorescence microscopy is a relatively rapid and reliable method to determine self-incompatibility in fruit-tree species. It is based on observation of pollen-tube growth in the pistils. Pollen tubes stained with fluorochromes show fluorescence when exposed to ultraviolet light. Testing of the self-compatibility trait was carried out in 123 apricot cultivars using fluorescence microscopy. In self-compatible cultivars, in the majority of pistils (60-100%), the pollen tubes reached the ovary. In contrast, in self-incompatible cultivars, pollen tubes growth ceased in the style, with plugs forming at their tips. In these cultivars, pollen tubes rarely (0-30%) reached the base of the style. Although apricot cultivars of the European eco-geographical group are traditionally considered self-compatible, we identified many self-incompatible cultivars, especially among those originating from new North American and West European breeding programs. About half ( 62) of the studied cultivars were self-incompatible. Given that self-incompatibility occurs frequently among new apricot cultivars, special care should be taken when considering cultivar composition in new orchard plantings. PB - Int Soc Horticultural Science, Leuven 1 C3 - XVI International Symposium on Apricot Breeding and Culture T1 - Fluorescence microscopy as a tool for determining self-incompatibility in apricot cultivars EP - 13 SP - 7 VL - 1214 DO - 10.17660/ActaHortic.2018.1214.2 ER -
@conference{ author = "Milatović, Dragan and Nikolić, D. and Radović, Aleksandar and Krska, B.", year = "2018", abstract = "Fluorescence microscopy is a relatively rapid and reliable method to determine self-incompatibility in fruit-tree species. It is based on observation of pollen-tube growth in the pistils. Pollen tubes stained with fluorochromes show fluorescence when exposed to ultraviolet light. Testing of the self-compatibility trait was carried out in 123 apricot cultivars using fluorescence microscopy. In self-compatible cultivars, in the majority of pistils (60-100%), the pollen tubes reached the ovary. In contrast, in self-incompatible cultivars, pollen tubes growth ceased in the style, with plugs forming at their tips. In these cultivars, pollen tubes rarely (0-30%) reached the base of the style. Although apricot cultivars of the European eco-geographical group are traditionally considered self-compatible, we identified many self-incompatible cultivars, especially among those originating from new North American and West European breeding programs. About half ( 62) of the studied cultivars were self-incompatible. Given that self-incompatibility occurs frequently among new apricot cultivars, special care should be taken when considering cultivar composition in new orchard plantings.", publisher = "Int Soc Horticultural Science, Leuven 1", journal = "XVI International Symposium on Apricot Breeding and Culture", title = "Fluorescence microscopy as a tool for determining self-incompatibility in apricot cultivars", pages = "13-7", volume = "1214", doi = "10.17660/ActaHortic.2018.1214.2" }
Milatović, D., Nikolić, D., Radović, A.,& Krska, B.. (2018). Fluorescence microscopy as a tool for determining self-incompatibility in apricot cultivars. in XVI International Symposium on Apricot Breeding and Culture Int Soc Horticultural Science, Leuven 1., 1214, 7-13. https://doi.org/10.17660/ActaHortic.2018.1214.2
Milatović D, Nikolić D, Radović A, Krska B. Fluorescence microscopy as a tool for determining self-incompatibility in apricot cultivars. in XVI International Symposium on Apricot Breeding and Culture. 2018;1214:7-13. doi:10.17660/ActaHortic.2018.1214.2 .
Milatović, Dragan, Nikolić, D., Radović, Aleksandar, Krska, B., "Fluorescence microscopy as a tool for determining self-incompatibility in apricot cultivars" in XVI International Symposium on Apricot Breeding and Culture, 1214 (2018):7-13, https://doi.org/10.17660/ActaHortic.2018.1214.2 . .