In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks
2017
Authors
Skocajić, Dragana M.Nesić, Marija M.
Nonić, Marina Z.
Fotirić-Akšić, Milica
Grbić, Mihailo N.
Dukić, Matilda S.
Sijacić-Nikolić, Mirjana T.
Article (Published version)
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Several in vitro biotechnological techniques have been developed, all of which require a reliable protocol to produce a responsive callus mass. One of these techniques is callus fusion in vitro, which is reliable for the early detection of (in)compatibility of scions and rootstocks. In this paper, the possibility to obtain friable callus tissues was explored by callus induction of adult tissues of Japanese flowering cherry trees from the group Sato zakura (Prunus serrulata 'Amanogawa', 'Kanzan' and 'Kiku-shidare-zakura') and two domestic cherry rootstocks -Prunus avium and Prunus 'Colt'. The explants used in the research were: leaf petiole, leaf base with a part of a petiole, part of lamina with a midvein and a stem with an axillary bud. Among three plant growth media (MS, SH and WP) that were used in this study, the MS proved to be the most favourable for the majority of taxa during the callus induction process. For the sweet cherry tree and the cultivars 'Kanzan' and 'Colt', the SH p...lant growth medium was also acceptable. The best results in callogenesis were obtained for the majority of taxons with auxin at the concentration 2 mgL-1 NAA and cytokinin BAP 0.5 mgL-1. It is also possible to use 2.4-D at the same concentration as a substitute for the genotypes Prunus avium, Prunus ` Colt' and Prunus serrulata 'Kanzan', whereas IBA proved to be an inappropriate auxin for callus induction. The protocol described herein is proved to be efficient callus induction in a range of taxa of genus Prunus.
Keywords:
callogenesis / explants / growth media / in vitro / Prunus serrulata / scionSource:
NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA, 2017, 45, 2, 392-399Publisher:
- Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca
Funding / projects:
- Studying climate change and its influence on environment: impacts, adaptation and mitigation (RS-43007)
DOI: 10.15835/nbha45210899
ISSN: 0255-965X
WoS: 000411504400011
Scopus: 2-s2.0-85029455973
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Poljoprivredni fakultetTY - JOUR AU - Skocajić, Dragana M. AU - Nesić, Marija M. AU - Nonić, Marina Z. AU - Fotirić-Akšić, Milica AU - Grbić, Mihailo N. AU - Dukić, Matilda S. AU - Sijacić-Nikolić, Mirjana T. PY - 2017 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4490 AB - Several in vitro biotechnological techniques have been developed, all of which require a reliable protocol to produce a responsive callus mass. One of these techniques is callus fusion in vitro, which is reliable for the early detection of (in)compatibility of scions and rootstocks. In this paper, the possibility to obtain friable callus tissues was explored by callus induction of adult tissues of Japanese flowering cherry trees from the group Sato zakura (Prunus serrulata 'Amanogawa', 'Kanzan' and 'Kiku-shidare-zakura') and two domestic cherry rootstocks -Prunus avium and Prunus 'Colt'. The explants used in the research were: leaf petiole, leaf base with a part of a petiole, part of lamina with a midvein and a stem with an axillary bud. Among three plant growth media (MS, SH and WP) that were used in this study, the MS proved to be the most favourable for the majority of taxa during the callus induction process. For the sweet cherry tree and the cultivars 'Kanzan' and 'Colt', the SH plant growth medium was also acceptable. The best results in callogenesis were obtained for the majority of taxons with auxin at the concentration 2 mgL-1 NAA and cytokinin BAP 0.5 mgL-1. It is also possible to use 2.4-D at the same concentration as a substitute for the genotypes Prunus avium, Prunus ` Colt' and Prunus serrulata 'Kanzan', whereas IBA proved to be an inappropriate auxin for callus induction. The protocol described herein is proved to be efficient callus induction in a range of taxa of genus Prunus. PB - Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca T2 - NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA T1 - In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks EP - 399 IS - 2 SP - 392 VL - 45 DO - 10.15835/nbha45210899 ER -
@article{ author = "Skocajić, Dragana M. and Nesić, Marija M. and Nonić, Marina Z. and Fotirić-Akšić, Milica and Grbić, Mihailo N. and Dukić, Matilda S. and Sijacić-Nikolić, Mirjana T.", year = "2017", abstract = "Several in vitro biotechnological techniques have been developed, all of which require a reliable protocol to produce a responsive callus mass. One of these techniques is callus fusion in vitro, which is reliable for the early detection of (in)compatibility of scions and rootstocks. In this paper, the possibility to obtain friable callus tissues was explored by callus induction of adult tissues of Japanese flowering cherry trees from the group Sato zakura (Prunus serrulata 'Amanogawa', 'Kanzan' and 'Kiku-shidare-zakura') and two domestic cherry rootstocks -Prunus avium and Prunus 'Colt'. The explants used in the research were: leaf petiole, leaf base with a part of a petiole, part of lamina with a midvein and a stem with an axillary bud. Among three plant growth media (MS, SH and WP) that were used in this study, the MS proved to be the most favourable for the majority of taxa during the callus induction process. For the sweet cherry tree and the cultivars 'Kanzan' and 'Colt', the SH plant growth medium was also acceptable. The best results in callogenesis were obtained for the majority of taxons with auxin at the concentration 2 mgL-1 NAA and cytokinin BAP 0.5 mgL-1. It is also possible to use 2.4-D at the same concentration as a substitute for the genotypes Prunus avium, Prunus ` Colt' and Prunus serrulata 'Kanzan', whereas IBA proved to be an inappropriate auxin for callus induction. The protocol described herein is proved to be efficient callus induction in a range of taxa of genus Prunus.", publisher = "Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca", journal = "NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA", title = "In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks", pages = "399-392", number = "2", volume = "45", doi = "10.15835/nbha45210899" }
Skocajić, D. M., Nesić, M. M., Nonić, M. Z., Fotirić-Akšić, M., Grbić, M. N., Dukić, M. S.,& Sijacić-Nikolić, M. T.. (2017). In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks. in NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca., 45(2), 392-399. https://doi.org/10.15835/nbha45210899
Skocajić DM, Nesić MM, Nonić MZ, Fotirić-Akšić M, Grbić MN, Dukić MS, Sijacić-Nikolić MT. In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks. in NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA. 2017;45(2):392-399. doi:10.15835/nbha45210899 .
Skocajić, Dragana M., Nesić, Marija M., Nonić, Marina Z., Fotirić-Akšić, Milica, Grbić, Mihailo N., Dukić, Matilda S., Sijacić-Nikolić, Mirjana T., "In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks" in NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA, 45, no. 2 (2017):392-399, https://doi.org/10.15835/nbha45210899 . .