Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism
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Pavlović, Vera P.
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Modern, efficient, and cost-effective approach to remediation of heavy metal-contaminated soil is based on the application of microorganisms. In this paper, four isolates from agricultural and urban contaminated soil showed abundant growth in the presence of copper(II) sulfate pentahydrate (CuSO4 center dot 5H(2)O) up to 2 mM. Selected yeasts were identified by molecular methods as Candida tropicalis (three isolates) and Schwanniomyces occidentalis (one isolate). C. tropicalis (4TD1101S) showed the highest percentage of bioaccumulation capabilities (94.37%), determined by the inductively coupled plasma optical emission spectrometry (ICP-OES). The Raman spectra of C. tropicalis (4TD1101S) analyzed in a medium with the addition of 2 mM CuSO4 center dot 5H(2)O showed certain increase in metallothionein production, which represents a specific response of the yeast species to the stress conditions. These results indicate that soil yeasts represent a potential for practical application in th...e bioremediation of contaminated environments.
Кључне речи:Yeasts / Copper / Bioremediation / Candida tropicalis / Raman spectra / Bioaccumulation
Извор:Environmental Science and Pollution Research, 2017, 24, 27, 21885-21893
- Springer Heidelberg, Heidelberg