Acaricidal activity and sublethal effects of the microbial pesticide spinosad on Tetranychus urticae (Acari: Tetranychidae)
Само за регистроване кориснике
2017
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Laboratory bioassays were conducted to evaluate the toxicity of the microbial pesticide spinosad to different life stages of the two-spotted spider mite, Tetranychus urticae Koch, as well as its sublethal effects on reproduction and population growth of this important mite pest. The biopesticide was applied to bean primary leaves or leaf discs carrying spider mites using a Potter spray tower (2.7 mg/cm(2) aqueous deposit). The following LC50 and LC90 (mg/L) estimates for motile stages were obtained in acute toxicity bioassays: 27.52 and 116.72 (larvae), 36.55 and 136.20 (protonymphs), 82.76 and 721.28 (female deutonymphs), and 61.47 and 457.21 (adult females). Spinosad showed no significant ovicidal action: toxic effect observed after spraying eggs (LC50 = 105.78 mg/L, LC90 = 596.95 mg/L) was the result of its residual action on larvae that hatched from the treated eggs. The effects of spinosad on life history traits and population growth of adult female survivors from treatments with ...240, 120 and 60 mg/L were evaluated in two successive 7-day bioassays on untreated leaf discs. In the first bioassay, females that survived treatments as 24 h old eggs and completed their juvenile development on treated leaves had significantly lower gross fecundity, net fecundity and instantaneous rate of increase (r(i)) but the reduction was merely 4-6%, 9-11%, and 2-3%, respectively. Female longevity was significantly reduced (approximately by half a day) only after treatment with 240 mg/L. In the second bioassay, in which females were treated during their pre-ovipositional period, the treatments with 240 and 120 mg/L significantly reduced their gross fecundity (16-17%), net fecundity (28-31%), ri values (8-9%) and female longevity (approximately by one day). Spinosad effects on the intrinsic rate of increase (rm) and other demographic parameters were evaluated in two successive bioassays in which life tables were constructed for females that survived treatment with 120 mg/L at the egg stage (first demographic bioassay) or pre-ovipositional period (second demographic bioassay). In the first bioassay, the intrinsic rate of increase was significantly higher in treated (r(m) = 0.278) than control mites (r(m) = 0.267) as a result of higher net fertility at the beginning of reproduction of treated females. In the second bioassay, treated females had significantly lower rm than control females (0.254 and 0.283, respectively). The results obtained in this study indicate that spinosad, applied against insect pests (at field relevant rates of 60-240 mg/L), could eliminate a part of T. urticae population as well, but survivors would retain a significant potential for population recovery.
Кључне речи:
T. urticae / spinosad / toxicity / life history traits / population growthИзвор:
Systematic and Applied Acarology, 2017, 22, 10, 1748-1762Издавач:
- Systematic & Applied Acarology Soc London, Natural History Museum, London
Финансирање / пројекти:
- Проучавање биљних патогена, артропода, корова и пестицида у циљу развоја метода биорационалне заштите биља и производње безбедне хране (RS-MESTD-Technological Development (TD or TR)-31043)
DOI: 10.11158/saa.22.10.14
ISSN: 1362-1971
WoS: 000412773300014
Scopus: 2-s2.0-85030767271
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Medo, Irena AU - Stojnić, Bojan AU - Marcić, Dejan PY - 2017 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4478 AB - Laboratory bioassays were conducted to evaluate the toxicity of the microbial pesticide spinosad to different life stages of the two-spotted spider mite, Tetranychus urticae Koch, as well as its sublethal effects on reproduction and population growth of this important mite pest. The biopesticide was applied to bean primary leaves or leaf discs carrying spider mites using a Potter spray tower (2.7 mg/cm(2) aqueous deposit). The following LC50 and LC90 (mg/L) estimates for motile stages were obtained in acute toxicity bioassays: 27.52 and 116.72 (larvae), 36.55 and 136.20 (protonymphs), 82.76 and 721.28 (female deutonymphs), and 61.47 and 457.21 (adult females). Spinosad showed no significant ovicidal action: toxic effect observed after spraying eggs (LC50 = 105.78 mg/L, LC90 = 596.95 mg/L) was the result of its residual action on larvae that hatched from the treated eggs. The effects of spinosad on life history traits and population growth of adult female survivors from treatments with 240, 120 and 60 mg/L were evaluated in two successive 7-day bioassays on untreated leaf discs. In the first bioassay, females that survived treatments as 24 h old eggs and completed their juvenile development on treated leaves had significantly lower gross fecundity, net fecundity and instantaneous rate of increase (r(i)) but the reduction was merely 4-6%, 9-11%, and 2-3%, respectively. Female longevity was significantly reduced (approximately by half a day) only after treatment with 240 mg/L. In the second bioassay, in which females were treated during their pre-ovipositional period, the treatments with 240 and 120 mg/L significantly reduced their gross fecundity (16-17%), net fecundity (28-31%), ri values (8-9%) and female longevity (approximately by one day). Spinosad effects on the intrinsic rate of increase (rm) and other demographic parameters were evaluated in two successive bioassays in which life tables were constructed for females that survived treatment with 120 mg/L at the egg stage (first demographic bioassay) or pre-ovipositional period (second demographic bioassay). In the first bioassay, the intrinsic rate of increase was significantly higher in treated (r(m) = 0.278) than control mites (r(m) = 0.267) as a result of higher net fertility at the beginning of reproduction of treated females. In the second bioassay, treated females had significantly lower rm than control females (0.254 and 0.283, respectively). The results obtained in this study indicate that spinosad, applied against insect pests (at field relevant rates of 60-240 mg/L), could eliminate a part of T. urticae population as well, but survivors would retain a significant potential for population recovery. PB - Systematic & Applied Acarology Soc London, Natural History Museum, London T2 - Systematic and Applied Acarology T1 - Acaricidal activity and sublethal effects of the microbial pesticide spinosad on Tetranychus urticae (Acari: Tetranychidae) EP - 1762 IS - 10 SP - 1748 VL - 22 DO - 10.11158/saa.22.10.14 ER -
@article{ author = "Medo, Irena and Stojnić, Bojan and Marcić, Dejan", year = "2017", abstract = "Laboratory bioassays were conducted to evaluate the toxicity of the microbial pesticide spinosad to different life stages of the two-spotted spider mite, Tetranychus urticae Koch, as well as its sublethal effects on reproduction and population growth of this important mite pest. The biopesticide was applied to bean primary leaves or leaf discs carrying spider mites using a Potter spray tower (2.7 mg/cm(2) aqueous deposit). The following LC50 and LC90 (mg/L) estimates for motile stages were obtained in acute toxicity bioassays: 27.52 and 116.72 (larvae), 36.55 and 136.20 (protonymphs), 82.76 and 721.28 (female deutonymphs), and 61.47 and 457.21 (adult females). Spinosad showed no significant ovicidal action: toxic effect observed after spraying eggs (LC50 = 105.78 mg/L, LC90 = 596.95 mg/L) was the result of its residual action on larvae that hatched from the treated eggs. The effects of spinosad on life history traits and population growth of adult female survivors from treatments with 240, 120 and 60 mg/L were evaluated in two successive 7-day bioassays on untreated leaf discs. In the first bioassay, females that survived treatments as 24 h old eggs and completed their juvenile development on treated leaves had significantly lower gross fecundity, net fecundity and instantaneous rate of increase (r(i)) but the reduction was merely 4-6%, 9-11%, and 2-3%, respectively. Female longevity was significantly reduced (approximately by half a day) only after treatment with 240 mg/L. In the second bioassay, in which females were treated during their pre-ovipositional period, the treatments with 240 and 120 mg/L significantly reduced their gross fecundity (16-17%), net fecundity (28-31%), ri values (8-9%) and female longevity (approximately by one day). Spinosad effects on the intrinsic rate of increase (rm) and other demographic parameters were evaluated in two successive bioassays in which life tables were constructed for females that survived treatment with 120 mg/L at the egg stage (first demographic bioassay) or pre-ovipositional period (second demographic bioassay). In the first bioassay, the intrinsic rate of increase was significantly higher in treated (r(m) = 0.278) than control mites (r(m) = 0.267) as a result of higher net fertility at the beginning of reproduction of treated females. In the second bioassay, treated females had significantly lower rm than control females (0.254 and 0.283, respectively). The results obtained in this study indicate that spinosad, applied against insect pests (at field relevant rates of 60-240 mg/L), could eliminate a part of T. urticae population as well, but survivors would retain a significant potential for population recovery.", publisher = "Systematic & Applied Acarology Soc London, Natural History Museum, London", journal = "Systematic and Applied Acarology", title = "Acaricidal activity and sublethal effects of the microbial pesticide spinosad on Tetranychus urticae (Acari: Tetranychidae)", pages = "1762-1748", number = "10", volume = "22", doi = "10.11158/saa.22.10.14" }
Medo, I., Stojnić, B.,& Marcić, D.. (2017). Acaricidal activity and sublethal effects of the microbial pesticide spinosad on Tetranychus urticae (Acari: Tetranychidae). in Systematic and Applied Acarology Systematic & Applied Acarology Soc London, Natural History Museum, London., 22(10), 1748-1762. https://doi.org/10.11158/saa.22.10.14
Medo I, Stojnić B, Marcić D. Acaricidal activity and sublethal effects of the microbial pesticide spinosad on Tetranychus urticae (Acari: Tetranychidae). in Systematic and Applied Acarology. 2017;22(10):1748-1762. doi:10.11158/saa.22.10.14 .
Medo, Irena, Stojnić, Bojan, Marcić, Dejan, "Acaricidal activity and sublethal effects of the microbial pesticide spinosad on Tetranychus urticae (Acari: Tetranychidae)" in Systematic and Applied Acarology, 22, no. 10 (2017):1748-1762, https://doi.org/10.11158/saa.22.10.14 . .