Vitality and in vitro pollen germination of different 'Oblacinska' sour cherry clones

2017
Autori
Fotirić-Akšić, Milica
Cerović, Radosav
Rakonjac, Vera

Bakić, Ivana
Čolić, Slavica

Meland, Mekjell
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Vitality of pollen, in vitro pollen germination and pollen tube growth (pollen tube length and pollen tube growth rate) were investigated in Oblacinska sour cherry in order to determine the differences between clones which have divergent yielding potential. For this purpose two 'Oblacinska' sour cherry clones with high fruit set and high yields (II/2, III/9) and two with low fruit set and low-yielding (XI/3 and XIII/1) were used in this study. Pollen germination was done on artificial medium containing 14% sucrose and 0.3% agar-agar at room temperature (23 degrees C). Pollen tube growth was stopped with a drop of 40% formaldehyde, 1, 3, 6, 12 and 24 h after contact with the medium. The maximum percentage of germination ranged from 13.01% (clone II/2, after 1 h) to 54.19% (clone III/9, after 24 h). Pollen tube length varied from 64.84 mu m (clone XIII/1, after 1 h) to > 1,100 mu m (clones II/2 and III/9, after 24 h). Pollen growth rate was quite high (up to 1.71 mu m min(-1)) after 6 h ...of germination, but rather decreasing until 24 h of germination (0.56. 0.83 mu m min(-1)). The dynamics of in vitro pollen tubes growth among the clones were quite different, especially after 12 h and 24 h of germination. Clones that are singled out as fruitful (II/2 and III/9) gave much better results regarding pollen germination and pollen tube growth in comparison to clones which were characterized by low fruit set and yields (XI/3 and XIII/1).
Ključne reči:
Prunus cerasus L. / genotype / acetocarmine / germination medium / pollen tube growth / pollen growth rateIzvor:
Genetika, 2017, 49, 3, 791-800Izdavač:
- Društvo genetičara Srbije, Beograd
Finansiranje / projekti:
DOI: 10.2298/GENSR1703791A
ISSN: 0534-0012
WoS: 000423604800003
Scopus: 2-s2.0-85042130792
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Fotirić-Akšić, Milica AU - Cerović, Radosav AU - Rakonjac, Vera AU - Bakić, Ivana AU - Čolić, Slavica AU - Meland, Mekjell PY - 2017 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4439 AB - Vitality of pollen, in vitro pollen germination and pollen tube growth (pollen tube length and pollen tube growth rate) were investigated in Oblacinska sour cherry in order to determine the differences between clones which have divergent yielding potential. For this purpose two 'Oblacinska' sour cherry clones with high fruit set and high yields (II/2, III/9) and two with low fruit set and low-yielding (XI/3 and XIII/1) were used in this study. Pollen germination was done on artificial medium containing 14% sucrose and 0.3% agar-agar at room temperature (23 degrees C). Pollen tube growth was stopped with a drop of 40% formaldehyde, 1, 3, 6, 12 and 24 h after contact with the medium. The maximum percentage of germination ranged from 13.01% (clone II/2, after 1 h) to 54.19% (clone III/9, after 24 h). Pollen tube length varied from 64.84 mu m (clone XIII/1, after 1 h) to > 1,100 mu m (clones II/2 and III/9, after 24 h). Pollen growth rate was quite high (up to 1.71 mu m min(-1)) after 6 h of germination, but rather decreasing until 24 h of germination (0.56. 0.83 mu m min(-1)). The dynamics of in vitro pollen tubes growth among the clones were quite different, especially after 12 h and 24 h of germination. Clones that are singled out as fruitful (II/2 and III/9) gave much better results regarding pollen germination and pollen tube growth in comparison to clones which were characterized by low fruit set and yields (XI/3 and XIII/1). PB - Društvo genetičara Srbije, Beograd T2 - Genetika T1 - Vitality and in vitro pollen germination of different 'Oblacinska' sour cherry clones EP - 800 IS - 3 SP - 791 VL - 49 DO - 10.2298/GENSR1703791A ER -
@article{ author = "Fotirić-Akšić, Milica and Cerović, Radosav and Rakonjac, Vera and Bakić, Ivana and Čolić, Slavica and Meland, Mekjell", year = "2017", abstract = "Vitality of pollen, in vitro pollen germination and pollen tube growth (pollen tube length and pollen tube growth rate) were investigated in Oblacinska sour cherry in order to determine the differences between clones which have divergent yielding potential. For this purpose two 'Oblacinska' sour cherry clones with high fruit set and high yields (II/2, III/9) and two with low fruit set and low-yielding (XI/3 and XIII/1) were used in this study. Pollen germination was done on artificial medium containing 14% sucrose and 0.3% agar-agar at room temperature (23 degrees C). Pollen tube growth was stopped with a drop of 40% formaldehyde, 1, 3, 6, 12 and 24 h after contact with the medium. The maximum percentage of germination ranged from 13.01% (clone II/2, after 1 h) to 54.19% (clone III/9, after 24 h). Pollen tube length varied from 64.84 mu m (clone XIII/1, after 1 h) to > 1,100 mu m (clones II/2 and III/9, after 24 h). Pollen growth rate was quite high (up to 1.71 mu m min(-1)) after 6 h of germination, but rather decreasing until 24 h of germination (0.56. 0.83 mu m min(-1)). The dynamics of in vitro pollen tubes growth among the clones were quite different, especially after 12 h and 24 h of germination. Clones that are singled out as fruitful (II/2 and III/9) gave much better results regarding pollen germination and pollen tube growth in comparison to clones which were characterized by low fruit set and yields (XI/3 and XIII/1).", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika", title = "Vitality and in vitro pollen germination of different 'Oblacinska' sour cherry clones", pages = "800-791", number = "3", volume = "49", doi = "10.2298/GENSR1703791A" }
Fotirić-Akšić, M., Cerović, R., Rakonjac, V., Bakić, I., Čolić, S.,& Meland, M.. (2017). Vitality and in vitro pollen germination of different 'Oblacinska' sour cherry clones. in Genetika Društvo genetičara Srbije, Beograd., 49(3), 791-800. https://doi.org/10.2298/GENSR1703791A
Fotirić-Akšić M, Cerović R, Rakonjac V, Bakić I, Čolić S, Meland M. Vitality and in vitro pollen germination of different 'Oblacinska' sour cherry clones. in Genetika. 2017;49(3):791-800. doi:10.2298/GENSR1703791A .
Fotirić-Akšić, Milica, Cerović, Radosav, Rakonjac, Vera, Bakić, Ivana, Čolić, Slavica, Meland, Mekjell, "Vitality and in vitro pollen germination of different 'Oblacinska' sour cherry clones" in Genetika, 49, no. 3 (2017):791-800, https://doi.org/10.2298/GENSR1703791A . .