Antifungal-demelanizing properties and RAW264.7 macrophages stimulation of glucan sulfate from the mycelium of the mushroom Ganoderma lucidum
Abstract
Underutilized mycelium of Ganoderma lucidum BCCM 31549 has been a significant source of a glucan sulfate (GS) possessing therapeutic activities. GS have been evaluated for their antifungaldemelanizing properties and nitrite oxide production from stimulated RAW264.7 macrophages. GS exhibited antifungal activity against Aspergillus niger A60 with a minimum inhibitory concentration of 60 mg/mL and a minimum fungicidal concentration of 100 mg/mL. At 60mg/mL (sublethal) and 30mg/mL (subinhibitory) doses of GS, the mycelium of A. niger A60 was successfully demelanized with a conidiophore head and black pigment reduction. Additionally, GS successfully stimulated RAW264.7 macrophage cells at a concentration of 500 mu g/mL to produce 0.45 mu M of nitric oxide. The GS-stimulated RAW264.7 macrophages were morphologically similar to those treated with lipopolysaccharide. The results highlight a novel bifunctional property of mycelial GS from G. lucidum.
Keywords:
antifungal activity / demelanization / Ganoderma lucidum / glucan sulfate / nitric oxide productionSource:
Food Science and Biotechnology, 2017, 26, 1, 159-165Publisher:
- Korean Society Food Science & Technology-Kosfost, Seoul
Funding / projects:
- Majlis Amanah Rakyat Malaysia
DOI: 10.1007/s10068-017-0021-6
ISSN: 1226-7708
PubMed: 30263523
WoS: 000396268200021
Scopus: 2-s2.0-85014896461
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Institution/Community
Poljoprivredni fakultetTY - JOUR AU - Wan-Mohtar, Wan Abd Al Qadr Imad AU - Viegelmann, Christina AU - Klaus, Anita AU - Lim, Sarina Abdul Halim PY - 2017 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4379 AB - Underutilized mycelium of Ganoderma lucidum BCCM 31549 has been a significant source of a glucan sulfate (GS) possessing therapeutic activities. GS have been evaluated for their antifungaldemelanizing properties and nitrite oxide production from stimulated RAW264.7 macrophages. GS exhibited antifungal activity against Aspergillus niger A60 with a minimum inhibitory concentration of 60 mg/mL and a minimum fungicidal concentration of 100 mg/mL. At 60mg/mL (sublethal) and 30mg/mL (subinhibitory) doses of GS, the mycelium of A. niger A60 was successfully demelanized with a conidiophore head and black pigment reduction. Additionally, GS successfully stimulated RAW264.7 macrophage cells at a concentration of 500 mu g/mL to produce 0.45 mu M of nitric oxide. The GS-stimulated RAW264.7 macrophages were morphologically similar to those treated with lipopolysaccharide. The results highlight a novel bifunctional property of mycelial GS from G. lucidum. PB - Korean Society Food Science & Technology-Kosfost, Seoul T2 - Food Science and Biotechnology T1 - Antifungal-demelanizing properties and RAW264.7 macrophages stimulation of glucan sulfate from the mycelium of the mushroom Ganoderma lucidum EP - 165 IS - 1 SP - 159 VL - 26 DO - 10.1007/s10068-017-0021-6 ER -
@article{ author = "Wan-Mohtar, Wan Abd Al Qadr Imad and Viegelmann, Christina and Klaus, Anita and Lim, Sarina Abdul Halim", year = "2017", abstract = "Underutilized mycelium of Ganoderma lucidum BCCM 31549 has been a significant source of a glucan sulfate (GS) possessing therapeutic activities. GS have been evaluated for their antifungaldemelanizing properties and nitrite oxide production from stimulated RAW264.7 macrophages. GS exhibited antifungal activity against Aspergillus niger A60 with a minimum inhibitory concentration of 60 mg/mL and a minimum fungicidal concentration of 100 mg/mL. At 60mg/mL (sublethal) and 30mg/mL (subinhibitory) doses of GS, the mycelium of A. niger A60 was successfully demelanized with a conidiophore head and black pigment reduction. Additionally, GS successfully stimulated RAW264.7 macrophage cells at a concentration of 500 mu g/mL to produce 0.45 mu M of nitric oxide. The GS-stimulated RAW264.7 macrophages were morphologically similar to those treated with lipopolysaccharide. The results highlight a novel bifunctional property of mycelial GS from G. lucidum.", publisher = "Korean Society Food Science & Technology-Kosfost, Seoul", journal = "Food Science and Biotechnology", title = "Antifungal-demelanizing properties and RAW264.7 macrophages stimulation of glucan sulfate from the mycelium of the mushroom Ganoderma lucidum", pages = "165-159", number = "1", volume = "26", doi = "10.1007/s10068-017-0021-6" }
Wan-Mohtar, W. A. A. Q. I., Viegelmann, C., Klaus, A.,& Lim, S. A. H.. (2017). Antifungal-demelanizing properties and RAW264.7 macrophages stimulation of glucan sulfate from the mycelium of the mushroom Ganoderma lucidum. in Food Science and Biotechnology Korean Society Food Science & Technology-Kosfost, Seoul., 26(1), 159-165. https://doi.org/10.1007/s10068-017-0021-6
Wan-Mohtar WAAQI, Viegelmann C, Klaus A, Lim SAH. Antifungal-demelanizing properties and RAW264.7 macrophages stimulation of glucan sulfate from the mycelium of the mushroom Ganoderma lucidum. in Food Science and Biotechnology. 2017;26(1):159-165. doi:10.1007/s10068-017-0021-6 .
Wan-Mohtar, Wan Abd Al Qadr Imad, Viegelmann, Christina, Klaus, Anita, Lim, Sarina Abdul Halim, "Antifungal-demelanizing properties and RAW264.7 macrophages stimulation of glucan sulfate from the mycelium of the mushroom Ganoderma lucidum" in Food Science and Biotechnology, 26, no. 1 (2017):159-165, https://doi.org/10.1007/s10068-017-0021-6 . .