Phenotypic and molecular evaluation of genetic diversity in ns safflower (Carthamus tinctorius L.): Collection

Abstract

Safflower (Carthamus tinctorius L.) belongs to the Asteraceae (Compositae) family. It is primarily grown for seeds used for bird feed or as edible oil. Stamens are used in traditional medicine and nutrition. Breeding for high resistance to dry growing conditions has initiated intensive studies of this plant species in recent years. Six safflower genotypes of different geographical origins (Ukraine, Italy, Turkey) were collected and added to the collection of less cultivated oil plant species of the Institute of Field and Vegetable Crops in Novi Sad. Phenotypic observations during two growing seasons revealed that analysed genotypes differed in flower colour (yellow, orange, red), in the presence of spines, and in seed oil and protein content. Oil and protein content differed between years and genotypes, indicating large influence of genotype and environmental conditions on variations of these quantitative traits that are negatively correlated. Genetic variability of the analysed genotypes was tested by use of molecular markers. Given that sunflower and safflower belong to the same family, the possibility of applying SSR markers developed for sunflower for molecular analysis of safflower was analysed. The obtained results proved that sunflower markers can be successfully transferred to safflower. Future studies should include larger number of markers in order to identify polymorphic and informative ones. Significant variations within a relatively small number of the analysed safflower genotypes justify further work on the evaluation of the collection, taking into account both genetic and environmental factors.

Šafranjika (Carthamus tinctorius L.) pripada porodici Asteraceae (Composita). Gaji se prvenstveno za dobijanje semena koje se koristi za ishranu ptica ili proizvodnju jestivog ulja. Prašnici se koriste u tradicionalnoj medicini i ishrani. Visoka otpornost na sušne uslove gajenja inicirala je poslednjih godina intenzivnija istraživanja ove biljne vrste. Za kolekciju manje gajenih uljanih biljnih vrsta Instituta za ratarstvo i povrtarstvo, prikupljeno je šest genotipova šafranjike različitog geografskog porekla (Ukrajina, Italija, Turska). Fenotipskim opažanjem u toku dve vegetacione sezone utvrđeno je da se genotipovi među sobom razlikuju u boji cveta (žuta, narandžasta, crvena), u prisustvu bodlji, kao i sadržaju ulja i proteina u semenu. Sadržaj ulja i proteina se razlikovao između godina i genotipova, što ukazuje na veliki uticaj i genotipa i spoljašnje sredine u variranju ovih kvantitativnih svojstava koja se nalaze u negativnoj korelaciji. Genetička varijabilnost genotipova je ispitana molekularnim markerima. S obzirom na to da suncokret i šafranjika pripadaju istoj porodici, proučavana je mogućnost upotrebe SSR markera suncokreta za molekularnu analizu šafranjike. Dobijeni rezultati pokazali su da se markeri suncokreta mogu uspešno koristiti za šafranjiku i da buduća istraživanja treba da sadrže veći broj markera u cilju identifikovanja polimorfnih i informativnih markera. Značajne razlike u relativno malom broju analiziranih genotipova šafranjike opravdavaju dalji rad na evaluaciji kolekcije, uzimajući u obzir i uslove sredine i genetičku varijabilnost.