The effect of temperature on pollen germination and pollen tube growth of pear cultivars

Abstract

The effect of three different temperatures (8, 16 and 24°C) on pollen germination and length of pollen tube in vitro was investigated in four pear cultivars ('Butirra Precoce Morettini', 'Williams', 'Conference' and 'Abate Fetel'). The temperature showed a significant effect on in vitro pollen germination. The highest pollen germination was determined at a temperature of 24°C (53.25%), somewhat lower at 16°C (44.72%) and the lowest at 8°C (23.16%). The temperature effect was significantly more pronounced on the length of pollen tube. Pollen tube length was about three times higher at the temperatures of 16 and 24°C compared to 8°C. The temperature of 8°C was not sufficient for pollen germination and pollen tube growth in pear cultivars. However, temperatures of 16 and 24°C were optimal for pollen germination and pollen tube growth.

U ovom radu ispitivan je uticaj tri različite temperature (8, 16 i 24°C) na klijavost polena i dužinu polenovih cevčica kod četiri sorte kruške (Rana Moretinijeva, Vilijamovka, Konferans i Fetelova). Temperatura je ispoljila značajan uticaj na klijavost polena. Najveća klijavost polena je utvrđena na temperaturi od 24°C (53,25%), nešto manja na 16°C (44,72%), a najmanja na 8°C (23,16%). Uticaj temperature je bio znatno više izražen na dužinu polenovih cevčica. Dužina polenovih cevčica je za oko tri puta bila veća na temperaturama od 16 i 24°C u odnosu na 8°C. Temperatura od 8°C nije dovoljna za klijavost polena i rast polenovih cevčica sorti kruške. Međutim, temperature od 16 i 24°C su optimalne za klijavost polena i rast polenovih cevčica.