Raman spectroscopy and determination of soil yeasts
Raman spektroskopija i determinacija zemljišnih kvasaca
Апстракт
In this paper, two isolates of yeasts, members of the genus Candida, have been examined using Raman spectroscopy. The yeasts were isolated from soils sampled at the town park in Tivat (Montenegro) and experimental farm Radmilovac (Serbia), by the accumulation method. The pure cultures of yeasts were identified by the API 20C AUX system. The presence of Candida guilliermondii and Candida utilis was noted. The Raman spectra, originating from lipids, amides, proteins, carbohydrates, aromatic amino acids and the nucleotide bases, were analyzed in the wide range of the wave numbers from 500 to 3200 cm-1. It was noticed that peaks which correspond to the lipid fraction were more pronounced in the case of Candida guilliermondii compared to Candida utilis. This may represent a specific response of the yeast species to stress conditions.
U ovom radu su Raman spektroskopijom ispitivana dva izolata kvasaca iz roda Candida. Izolacija kvasaca je izvršena iz zemljišta uzetog iz gradskog parka u Tivtu (Crna Gora) i sa oglednog dobra Radmilovac (Poljoprivredni fakultet, Beograd, Srbija), metodom nakupljanja. Dobijene čiste kulture kvasaca identifikovane su primenom API AUX 20C sistema (bioMerieux-Vitek). Konstatovano je prisustvo vrsta Candida guilliermondii i Candida utilis. Analizirani su Raman spektri koji potiču od lipida, amida, proteina, ugljenih hidrata, aromatičnih aminokiselina i nukleotidnih baza, u širokoj oblasti talasnih brojeva, od 500 do 3200 cm-1. Utvrđeno je da su kod kvasca Candida guilliermondii pikovi koji odgovaraju frakciji lipida bitno izraženiji nego kod Candida utilis, što može predstavljati specifičan odgovor date vrste kvasca na stres.
Кључне речи:
Raman spectra / yeast / lipids / proteins / amides / aromatic amino acids / Raman spektri / kvasci / lipidi / proteini / amidi / aromatične aminokiselineИзвор:
Zaštita materijala, 2016, 57, 3, 455-459Издавач:
- Inženjersko društvo za koroziju, Beograd
Финансирање / пројекти:
- Усмерена синтеза, структура и својства мултифункционалних материјала (RS-172057)
- Биодиверзитет као потенцијал у екоремедијационим технологијама оштећених екосистема (RS-31080)
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Pavlović, Vera P. AU - Radić, Danka AU - Karličić, Vera AU - Lalević, Blažo AU - Lević, Steva AU - Raičević, Vera PY - 2016 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4232 AB - In this paper, two isolates of yeasts, members of the genus Candida, have been examined using Raman spectroscopy. The yeasts were isolated from soils sampled at the town park in Tivat (Montenegro) and experimental farm Radmilovac (Serbia), by the accumulation method. The pure cultures of yeasts were identified by the API 20C AUX system. The presence of Candida guilliermondii and Candida utilis was noted. The Raman spectra, originating from lipids, amides, proteins, carbohydrates, aromatic amino acids and the nucleotide bases, were analyzed in the wide range of the wave numbers from 500 to 3200 cm-1. It was noticed that peaks which correspond to the lipid fraction were more pronounced in the case of Candida guilliermondii compared to Candida utilis. This may represent a specific response of the yeast species to stress conditions. AB - U ovom radu su Raman spektroskopijom ispitivana dva izolata kvasaca iz roda Candida. Izolacija kvasaca je izvršena iz zemljišta uzetog iz gradskog parka u Tivtu (Crna Gora) i sa oglednog dobra Radmilovac (Poljoprivredni fakultet, Beograd, Srbija), metodom nakupljanja. Dobijene čiste kulture kvasaca identifikovane su primenom API AUX 20C sistema (bioMerieux-Vitek). Konstatovano je prisustvo vrsta Candida guilliermondii i Candida utilis. Analizirani su Raman spektri koji potiču od lipida, amida, proteina, ugljenih hidrata, aromatičnih aminokiselina i nukleotidnih baza, u širokoj oblasti talasnih brojeva, od 500 do 3200 cm-1. Utvrđeno je da su kod kvasca Candida guilliermondii pikovi koji odgovaraju frakciji lipida bitno izraženiji nego kod Candida utilis, što može predstavljati specifičan odgovor date vrste kvasca na stres. PB - Inženjersko društvo za koroziju, Beograd T2 - Zaštita materijala T1 - Raman spectroscopy and determination of soil yeasts T1 - Raman spektroskopija i determinacija zemljišnih kvasaca EP - 459 IS - 3 SP - 455 VL - 57 DO - 10.5937/ZasMat1603455P ER -
@article{ author = "Pavlović, Vera P. and Radić, Danka and Karličić, Vera and Lalević, Blažo and Lević, Steva and Raičević, Vera", year = "2016", abstract = "In this paper, two isolates of yeasts, members of the genus Candida, have been examined using Raman spectroscopy. The yeasts were isolated from soils sampled at the town park in Tivat (Montenegro) and experimental farm Radmilovac (Serbia), by the accumulation method. The pure cultures of yeasts were identified by the API 20C AUX system. The presence of Candida guilliermondii and Candida utilis was noted. The Raman spectra, originating from lipids, amides, proteins, carbohydrates, aromatic amino acids and the nucleotide bases, were analyzed in the wide range of the wave numbers from 500 to 3200 cm-1. It was noticed that peaks which correspond to the lipid fraction were more pronounced in the case of Candida guilliermondii compared to Candida utilis. This may represent a specific response of the yeast species to stress conditions., U ovom radu su Raman spektroskopijom ispitivana dva izolata kvasaca iz roda Candida. Izolacija kvasaca je izvršena iz zemljišta uzetog iz gradskog parka u Tivtu (Crna Gora) i sa oglednog dobra Radmilovac (Poljoprivredni fakultet, Beograd, Srbija), metodom nakupljanja. Dobijene čiste kulture kvasaca identifikovane su primenom API AUX 20C sistema (bioMerieux-Vitek). Konstatovano je prisustvo vrsta Candida guilliermondii i Candida utilis. Analizirani su Raman spektri koji potiču od lipida, amida, proteina, ugljenih hidrata, aromatičnih aminokiselina i nukleotidnih baza, u širokoj oblasti talasnih brojeva, od 500 do 3200 cm-1. Utvrđeno je da su kod kvasca Candida guilliermondii pikovi koji odgovaraju frakciji lipida bitno izraženiji nego kod Candida utilis, što može predstavljati specifičan odgovor date vrste kvasca na stres.", publisher = "Inženjersko društvo za koroziju, Beograd", journal = "Zaštita materijala", title = "Raman spectroscopy and determination of soil yeasts, Raman spektroskopija i determinacija zemljišnih kvasaca", pages = "459-455", number = "3", volume = "57", doi = "10.5937/ZasMat1603455P" }
Pavlović, V. P., Radić, D., Karličić, V., Lalević, B., Lević, S.,& Raičević, V.. (2016). Raman spectroscopy and determination of soil yeasts. in Zaštita materijala Inženjersko društvo za koroziju, Beograd., 57(3), 455-459. https://doi.org/10.5937/ZasMat1603455P
Pavlović VP, Radić D, Karličić V, Lalević B, Lević S, Raičević V. Raman spectroscopy and determination of soil yeasts. in Zaštita materijala. 2016;57(3):455-459. doi:10.5937/ZasMat1603455P .
Pavlović, Vera P., Radić, Danka, Karličić, Vera, Lalević, Blažo, Lević, Steva, Raičević, Vera, "Raman spectroscopy and determination of soil yeasts" in Zaštita materijala, 57, no. 3 (2016):455-459, https://doi.org/10.5937/ZasMat1603455P . .