Sunflower line with resistance for downy mildew (Ha-26R) and susceptible line (Ha-26S) were inoculated with the suspension of Plasmopara halstedii zoosporangia, race 730, in the phase of first pair of leaves. The expression of defense related genes was investigated in the time period of 2 to 96 h after treatment. Several categories of defense-related genes: signalization (EDS1 and EDR1); H2O2 producing (Hacaox and Haoxox); antioxidative response (SODc and SODp), pathogenesis related (chi, PAL, PR5) were examined. Most examined defense related genes were constitutive with higher expression in resistant line. However, in response to secondary downy mildew infection six genes were upregulated. Upregulation of HaEDS1 signaling gene 2 h after infection indicates that SA mediated response is activated. Pathogenesis related genes: chitinase and PR5, were also upregulated in the earliest time point. Other defense related genes: SODp, Caox and OxOx were sequentially upregulated from 4 to 48 h a...fter infection in resistant line. Our results indicate that the early response of defense related genes to secondary downy mildew infection, resembles to hypersensitive-like reaction and is connected with resistance conferred by Pl6 gene. According to our results resistance to secondary infection is characterized with earlier upregulation of PR5 in comparison to primary infection.
TY - JOUR
AU - Ćurčić, N.
AU - Prokić, Ljiljana
AU - Škorić, D.
AU - Panković, D.M.
PY - 2016
UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4217
AB - Sunflower line with resistance for downy mildew (Ha-26R) and susceptible line (Ha-26S) were inoculated with the suspension of Plasmopara halstedii zoosporangia, race 730, in the phase of first pair of leaves. The expression of defense related genes was investigated in the time period of 2 to 96 h after treatment. Several categories of defense-related genes: signalization (EDS1 and EDR1); H2O2 producing (Hacaox and Haoxox); antioxidative response (SODc and SODp), pathogenesis related (chi, PAL, PR5) were examined. Most examined defense related genes were constitutive with higher expression in resistant line. However, in response to secondary downy mildew infection six genes were upregulated. Upregulation of HaEDS1 signaling gene 2 h after infection indicates that SA mediated response is activated. Pathogenesis related genes: chitinase and PR5, were also upregulated in the earliest time point. Other defense related genes: SODp, Caox and OxOx were sequentially upregulated from 4 to 48 h after infection in resistant line. Our results indicate that the early response of defense related genes to secondary downy mildew infection, resembles to hypersensitive-like reaction and is connected with resistance conferred by Pl6 gene. According to our results resistance to secondary infection is characterized with earlier upregulation of PR5 in comparison to primary infection.
PB - Institut za ratarstvo i povrtarstvo, Novi Sad
T2 - Helia
T1 - Early response of defense related genes to secondary downy mildew infection in Sunflower line with Pl6 gene
EP - 182
IS - 65
SP - 169
VL - 39
DO - 10.1515/helia-2016-0009
ER -
@article{
author = "Ćurčić, N. and Prokić, Ljiljana and Škorić, D. and Panković, D.M.",
year = "2016",
abstract = "Sunflower line with resistance for downy mildew (Ha-26R) and susceptible line (Ha-26S) were inoculated with the suspension of Plasmopara halstedii zoosporangia, race 730, in the phase of first pair of leaves. The expression of defense related genes was investigated in the time period of 2 to 96 h after treatment. Several categories of defense-related genes: signalization (EDS1 and EDR1); H2O2 producing (Hacaox and Haoxox); antioxidative response (SODc and SODp), pathogenesis related (chi, PAL, PR5) were examined. Most examined defense related genes were constitutive with higher expression in resistant line. However, in response to secondary downy mildew infection six genes were upregulated. Upregulation of HaEDS1 signaling gene 2 h after infection indicates that SA mediated response is activated. Pathogenesis related genes: chitinase and PR5, were also upregulated in the earliest time point. Other defense related genes: SODp, Caox and OxOx were sequentially upregulated from 4 to 48 h after infection in resistant line. Our results indicate that the early response of defense related genes to secondary downy mildew infection, resembles to hypersensitive-like reaction and is connected with resistance conferred by Pl6 gene. According to our results resistance to secondary infection is characterized with earlier upregulation of PR5 in comparison to primary infection.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Helia",
title = "Early response of defense related genes to secondary downy mildew infection in Sunflower line with Pl6 gene",
pages = "182-169",
number = "65",
volume = "39",
doi = "10.1515/helia-2016-0009"
}
Ćurčić, N., Prokić, L., Škorić, D.,& Panković, D.M.. (2016). Early response of defense related genes to secondary downy mildew infection in Sunflower line with Pl6 gene. in Helia
Institut za ratarstvo i povrtarstvo, Novi Sad., 39(65), 169-182.
https://doi.org/10.1515/helia-2016-0009
Ćurčić N, Prokić L, Škorić D, Panković D. Early response of defense related genes to secondary downy mildew infection in Sunflower line with Pl6 gene. in Helia. 2016;39(65):169-182.
doi:10.1515/helia-2016-0009 .
Ćurčić, N., Prokić, Ljiljana, Škorić, D., Panković, D.M., "Early response of defense related genes to secondary downy mildew infection in Sunflower line with Pl6 gene" in Helia, 39, no. 65 (2016):169-182,
https://doi.org/10.1515/helia-2016-0009 . .
