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dc.creatorMirković, Nemanja
dc.creatorPolović, Natalija
dc.creatorVukotić, Goran
dc.creatorJovcić, Branko
dc.creatorMiljković, Marija
dc.creatorRadulović, Zorica
dc.creatorDiep, Dzung B.
dc.creatorKojić, Milan
dc.date.accessioned2020-12-17T21:45:38Z
dc.date.available2020-12-17T21:45:38Z
dc.date.issued2016
dc.identifier.issn0099-2240
dc.identifier.urihttp://aspace.agrif.bg.ac.rs/handle/123456789/4208
dc.description.abstractBacteriocin producers normally possess dedicated immunity systems to protect themselves from their own bacteriocins. Lactococcus lactis strains LMG2081 and BGBM50 are known as lactococcin G producers. However, BGBM50 was sensitive to LMG2081, which indicated that LMG2081 might produce additional bacteriocins that are not present in BGBM50. Therefore, whole-genome sequencing of the two strains was performed, and a lantibiotic operon (called lctLMG) was identified in LMG2081 but not in BGBM50. The lctLMG operon contains six open reading frames; the first three genes, lmgA, lmgM, and lmgT, are involved in the biosynthesis and export of bacteriocin, while the other three genes, lmgF, lmgE, and lmgG, are involved in lantibiotic immunity. Mutational analysis confirmed that the lctLMG operon is responsible for the additional antimicrobial activity. Specifically, site-directed mutation within this operon rendered LMG2081 inactive toward BGBM50. Subsequent purification and electrospray ionization-time of flight mass spectrometric analysis confirmed that the lantibiotic bacteriocin called lacticin LMG is exported as a 25-amino-acid peptide. Lacticin LMG is highly similar to the lacticin 481 group. It is interesting that a bacteriocin producer produces two different classes of bacteriocins, whose operons are located in the chromosome and a plasmid.en
dc.publisherAmer Soc Microbiology, Washington
dc.relationFederation of European Microbiological Societies (FEMS)
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173019/RS//
dc.rightsopenAccess
dc.sourceApplied and Environmental Microbiology
dc.titleLactococcus lactis LMG2081 Produces Two Bacteriocins, a Nonlantibiotic and a Novel Lantibioticen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage2562
dc.citation.issue8
dc.citation.other82(8): 2555-2562
dc.citation.rankM21
dc.citation.spage2555
dc.citation.volume82
dc.identifier.doi10.1128/AEM.03988-15
dc.identifier.fulltexthttp://aspace.agrif.bg.ac.rs/bitstream/id/2758/4205.pdf
dc.identifier.scopus2-s2.0-84963600251
dc.identifier.pmid26896142
dc.identifier.wos000373343300032
dc.type.versionpublishedVersion


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Приказ основних података о документу