Antioxidant (AO) capacity of various medicinal plants extracts and phenolic compounds was assessed by the most widely used spectrophotometric assays such as ferric reducing antioxidant power (FRAP) and scavenging of 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). In addition, two direct current (DC) polarographic assays, one based on a decrease of anodic current of [Hg(O2H)(OH)]-HydroxoPerhydroxoMercury(II) Complex (HPMC) formation in alkaline solution of H2O2, at the potential of mercury dissolution and another recently developed Mercury Reduction Antioxidant Power (MRAP), based on a a decrease of cathodic current of Hg(II) reduction were employed. Percentage of both currents decrease was plotted versus the volume of gradually added complex samples or the amount of individual ones and the slopes of these plots were used to express AO capacity. Total phenolic content (TPC) of extracts was determined by Folin-Ciocalteu (FC) assay. Co...rrelations between applied assays were calculated by regression analysis. Relative Antioxidant Capacity Index (RACI), calculated by assigning equal weight to all applied assays and Phenolic Antioxidant Coefficients (PAC), calculated as a ratio between particular AO capacity and TPC, were used to achieve more comprehensive comparison between analyzed samples, as well as applied assays.
TY - JOUR
AU - Petrović, Marija
AU - Suznjević, Desanka
AU - Pastor, Ferenc T.
AU - Veljović, Mile
AU - Pezo, Lato
AU - Antić, Mališa
AU - Gorjanović, Stanislava
PY - 2016
UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4140
AB - Antioxidant (AO) capacity of various medicinal plants extracts and phenolic compounds was assessed by the most widely used spectrophotometric assays such as ferric reducing antioxidant power (FRAP) and scavenging of 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). In addition, two direct current (DC) polarographic assays, one based on a decrease of anodic current of [Hg(O2H)(OH)]-HydroxoPerhydroxoMercury(II) Complex (HPMC) formation in alkaline solution of H2O2, at the potential of mercury dissolution and another recently developed Mercury Reduction Antioxidant Power (MRAP), based on a a decrease of cathodic current of Hg(II) reduction were employed. Percentage of both currents decrease was plotted versus the volume of gradually added complex samples or the amount of individual ones and the slopes of these plots were used to express AO capacity. Total phenolic content (TPC) of extracts was determined by Folin-Ciocalteu (FC) assay. Correlations between applied assays were calculated by regression analysis. Relative Antioxidant Capacity Index (RACI), calculated by assigning equal weight to all applied assays and Phenolic Antioxidant Coefficients (PAC), calculated as a ratio between particular AO capacity and TPC, were used to achieve more comprehensive comparison between analyzed samples, as well as applied assays.
PB - Bentham Science Publ Ltd, Sharjah
T2 - Combinatorial Chemistry & High Throughput Screening
T1 - Antioxidant Capacity Determination of Complex Samples and Individual Phenolics - Multilateral Approach
EP - 65
IS - 1
SP - 58
VL - 19
DO - 10.2174/1386207318666151102094227
ER -
@article{
author = "Petrović, Marija and Suznjević, Desanka and Pastor, Ferenc T. and Veljović, Mile and Pezo, Lato and Antić, Mališa and Gorjanović, Stanislava",
year = "2016",
abstract = "Antioxidant (AO) capacity of various medicinal plants extracts and phenolic compounds was assessed by the most widely used spectrophotometric assays such as ferric reducing antioxidant power (FRAP) and scavenging of 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). In addition, two direct current (DC) polarographic assays, one based on a decrease of anodic current of [Hg(O2H)(OH)]-HydroxoPerhydroxoMercury(II) Complex (HPMC) formation in alkaline solution of H2O2, at the potential of mercury dissolution and another recently developed Mercury Reduction Antioxidant Power (MRAP), based on a a decrease of cathodic current of Hg(II) reduction were employed. Percentage of both currents decrease was plotted versus the volume of gradually added complex samples or the amount of individual ones and the slopes of these plots were used to express AO capacity. Total phenolic content (TPC) of extracts was determined by Folin-Ciocalteu (FC) assay. Correlations between applied assays were calculated by regression analysis. Relative Antioxidant Capacity Index (RACI), calculated by assigning equal weight to all applied assays and Phenolic Antioxidant Coefficients (PAC), calculated as a ratio between particular AO capacity and TPC, were used to achieve more comprehensive comparison between analyzed samples, as well as applied assays.",
publisher = "Bentham Science Publ Ltd, Sharjah",
journal = "Combinatorial Chemistry & High Throughput Screening",
title = "Antioxidant Capacity Determination of Complex Samples and Individual Phenolics - Multilateral Approach",
pages = "65-58",
number = "1",
volume = "19",
doi = "10.2174/1386207318666151102094227"
}
Petrović, M., Suznjević, D., Pastor, F. T., Veljović, M., Pezo, L., Antić, M.,& Gorjanović, S.. (2016). Antioxidant Capacity Determination of Complex Samples and Individual Phenolics - Multilateral Approach. in Combinatorial Chemistry & High Throughput Screening
Bentham Science Publ Ltd, Sharjah., 19(1), 58-65.
https://doi.org/10.2174/1386207318666151102094227
Petrović M, Suznjević D, Pastor FT, Veljović M, Pezo L, Antić M, Gorjanović S. Antioxidant Capacity Determination of Complex Samples and Individual Phenolics - Multilateral Approach. in Combinatorial Chemistry & High Throughput Screening. 2016;19(1):58-65.
doi:10.2174/1386207318666151102094227 .
Petrović, Marija, Suznjević, Desanka, Pastor, Ferenc T., Veljović, Mile, Pezo, Lato, Antić, Mališa, Gorjanović, Stanislava, "Antioxidant Capacity Determination of Complex Samples and Individual Phenolics - Multilateral Approach" in Combinatorial Chemistry & High Throughput Screening, 19, no. 1 (2016):58-65,
https://doi.org/10.2174/1386207318666151102094227 . .
