Приказ основних података о документу
Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima
dc.creator | Zhu, Gengrui | |
dc.creator | Wang, Shunli | |
dc.creator | Zhen, Shoumin | |
dc.creator | Shen, Xixi | |
dc.creator | Prodanović, Slaven | |
dc.creator | Yan, Yueming | |
dc.date.accessioned | 2020-12-17T21:22:34Z | |
dc.date.available | 2020-12-17T21:22:34Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 0534-0012 | |
dc.identifier.uri | http://aspace.agrif.bg.ac.rs/handle/123456789/3839 | |
dc.description.abstract | Wheat related diploid species Ae. longissima (2n=2x=14, (SSl)-S-l) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S(l)-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S(l)x-123, 1S(l)x-129, 1S(l)x1-136, 1S(l)x2-136 and 1S(l)x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S(l)x-123, 2946 bp (979 aa) for 1S(l)x-129, 2901 bp (965 aa) for 1S(l)x1-136, 2982bp (991 aa) for 1S(l)x2-136 and 2928 bp (974 aa) for 1S(l)x2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1S(l)x-129 had an extra cysteine residue at the position 109 due to a TAT -> TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome. | en |
dc.publisher | Društvo genetičara Srbije, Beograd | |
dc.relation | National Natural Science Foundation of China (NSFC) [31271703, 31471485] | |
dc.relation | Natural Science Foundation of Beijing CityBeijing Natural Science Foundation | |
dc.relation | Key Developmental Project of Science and Technology | |
dc.relation | Beijing Municipal Commission of EducationBeijing Municipal Commission of Education [KZ201410028031] | |
dc.relation | National Key Project for Transgenic Crops of China [2011ZX08009-003-004] | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31069/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Genetika | |
dc.subject | Ae. longissima | en |
dc.subject | HMW-GS | en |
dc.subject | molecular clone | en |
dc.subject | phylogenetics | en |
dc.title | Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dc.citation.epage | 203 | |
dc.citation.issue | 1 | |
dc.citation.other | 47(1): 185-203 | |
dc.citation.rank | M23 | |
dc.citation.spage | 185 | |
dc.citation.volume | 47 | |
dc.identifier.doi | 10.2298/GENSR1501185Z | |
dc.identifier.fulltext | http://aspace.agrif.bg.ac.rs/bitstream/id/2396/3836.pdf | |
dc.identifier.scopus | 2-s2.0-84929323358 | |
dc.identifier.wos | 000355248900017 | |
dc.type.version | publishedVersion |