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dc.creatorZhu, Gengrui
dc.creatorWang, Shunli
dc.creatorZhen, Shoumin
dc.creatorShen, Xixi
dc.creatorProdanović, Slaven
dc.creatorYan, Yueming
dc.date.accessioned2020-12-17T21:22:34Z
dc.date.available2020-12-17T21:22:34Z
dc.date.issued2015
dc.identifier.issn0534-0012
dc.identifier.urihttp://aspace.agrif.bg.ac.rs/handle/123456789/3839
dc.description.abstractWheat related diploid species Ae. longissima (2n=2x=14, (SSl)-S-l) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S(l)-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S(l)x-123, 1S(l)x-129, 1S(l)x1-136, 1S(l)x2-136 and 1S(l)x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S(l)x-123, 2946 bp (979 aa) for 1S(l)x-129, 2901 bp (965 aa) for 1S(l)x1-136, 2982bp (991 aa) for 1S(l)x2-136 and 2928 bp (974 aa) for 1S(l)x2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1S(l)x-129 had an extra cysteine residue at the position 109 due to a TAT -> TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome.en
dc.publisherDruštvo genetičara Srbije, Beograd
dc.relationNational Natural Science Foundation of China (NSFC) [31271703, 31471485]
dc.relationNatural Science Foundation of Beijing CityBeijing Natural Science Foundation
dc.relationKey Developmental Project of Science and Technology
dc.relationBeijing Municipal Commission of EducationBeijing Municipal Commission of Education [KZ201410028031]
dc.relationNational Key Project for Transgenic Crops of China [2011ZX08009-003-004]
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31069/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceGenetika
dc.subjectAe. longissimaen
dc.subjectHMW-GSen
dc.subjectmolecular cloneen
dc.subjectphylogeneticsen
dc.titleMolecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissimaen
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage203
dc.citation.issue1
dc.citation.other47(1): 185-203
dc.citation.rankM23
dc.citation.spage185
dc.citation.volume47
dc.identifier.doi10.2298/GENSR1501185Z
dc.identifier.fulltexthttp://aspace.agrif.bg.ac.rs/bitstream/id/2396/3836.pdf
dc.identifier.rcubconv_5508
dc.identifier.scopus2-s2.0-84929323358
dc.identifier.wos000355248900017
dc.type.versionpublishedVersion


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