Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima
Апстракт
Wheat related diploid species Ae. longissima (2n=2x=14, (SSl)-S-l) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S(l)-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S(l)x-123, 1S(l)x-129, 1S(l)x1-136, 1S(l)x2-136 and 1S(l)x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S(l)x-123, 2946 bp (979 aa) for 1S(l)x-129, 2901 bp (965 aa) for 1S(l)x1-136, 2982bp (991 aa) for 1S(l)x2-136 and 2928 bp (974 aa) for 1S(l)x2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1S(l)x-129 had an extra cysteine residue at the position 109 due to a TAT -> TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these u...nusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome.
Кључне речи:
Ae. longissima / HMW-GS / molecular clone / phylogeneticsИзвор:
Genetika, 2015, 47, 1, 185-203Издавач:
- Društvo genetičara Srbije, Beograd
Финансирање / пројекти:
- National Natural Science Foundation of China (NSFC) [31271703, 31471485]
- Natural Science Foundation of Beijing CityBeijing Natural Science Foundation
- Key Developmental Project of Science and Technology
- Beijing Municipal Commission of EducationBeijing Municipal Commission of Education [KZ201410028031]
- National Key Project for Transgenic Crops of China [2011ZX08009-003-004]
- Коришћење биљних извора протеина, дијеталних влакана и антиоксиданаса у производњи хране (RS-31069)
DOI: 10.2298/GENSR1501185Z
ISSN: 0534-0012
WoS: 000355248900017
Scopus: 2-s2.0-84929323358
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Zhu, Gengrui AU - Wang, Shunli AU - Zhen, Shoumin AU - Shen, Xixi AU - Prodanović, Slaven AU - Yan, Yueming PY - 2015 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3839 AB - Wheat related diploid species Ae. longissima (2n=2x=14, (SSl)-S-l) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S(l)-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S(l)x-123, 1S(l)x-129, 1S(l)x1-136, 1S(l)x2-136 and 1S(l)x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S(l)x-123, 2946 bp (979 aa) for 1S(l)x-129, 2901 bp (965 aa) for 1S(l)x1-136, 2982bp (991 aa) for 1S(l)x2-136 and 2928 bp (974 aa) for 1S(l)x2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1S(l)x-129 had an extra cysteine residue at the position 109 due to a TAT -> TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome. PB - Društvo genetičara Srbije, Beograd T2 - Genetika T1 - Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima EP - 203 IS - 1 SP - 185 VL - 47 DO - 10.2298/GENSR1501185Z ER -
@article{ author = "Zhu, Gengrui and Wang, Shunli and Zhen, Shoumin and Shen, Xixi and Prodanović, Slaven and Yan, Yueming", year = "2015", abstract = "Wheat related diploid species Ae. longissima (2n=2x=14, (SSl)-S-l) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S(l)-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S(l)x-123, 1S(l)x-129, 1S(l)x1-136, 1S(l)x2-136 and 1S(l)x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S(l)x-123, 2946 bp (979 aa) for 1S(l)x-129, 2901 bp (965 aa) for 1S(l)x1-136, 2982bp (991 aa) for 1S(l)x2-136 and 2928 bp (974 aa) for 1S(l)x2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1S(l)x-129 had an extra cysteine residue at the position 109 due to a TAT -> TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika", title = "Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima", pages = "203-185", number = "1", volume = "47", doi = "10.2298/GENSR1501185Z" }
Zhu, G., Wang, S., Zhen, S., Shen, X., Prodanović, S.,& Yan, Y.. (2015). Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima. in Genetika Društvo genetičara Srbije, Beograd., 47(1), 185-203. https://doi.org/10.2298/GENSR1501185Z
Zhu G, Wang S, Zhen S, Shen X, Prodanović S, Yan Y. Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima. in Genetika. 2015;47(1):185-203. doi:10.2298/GENSR1501185Z .
Zhu, Gengrui, Wang, Shunli, Zhen, Shoumin, Shen, Xixi, Prodanović, Slaven, Yan, Yueming, "Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima" in Genetika, 47, no. 1 (2015):185-203, https://doi.org/10.2298/GENSR1501185Z . .