Evaluation of propidium monoazide real-time PCR for enumeration of probiotic lactobacilli microencapsulated in calcium alginate beads
Само за регистроване кориснике
2015
Аутори
Oketić, K.Bogović-Matijasić, B.
Obermajer, T.
Radulović, Zorica
Lević, Steva
Mirković, Nemanja
Nedović, Viktor
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of the study was to evaluate real-time PCR coupled with propidium monoazide (PMA) treatment for enumeration of microencapsulated probiotic lactobacilli microencapsulated in calcium alginate beads. Lactobacillus gasseri K7 (CCM 7710) and Lactobacillus delbrueckii subsp. bulgaricus (CCM 7712) were analysed by plate counting and PMA real-time PCR during storage at 4 degrees C for 90 days. PMA was effective in preventing PCR amplification of the target sequences of DNA released from heat-compromised bacteria. The values obtained by real-time PCR of non-treated samples were in general higher than those obtained by real-time PCR of PMA-treated samples or by plate counting, indicating the presence of sub-lethally injured cells. This study shows that plate count could not be completely replaced by culture independent method PMA real-time PCR for enumeration of probiotics, but may rather complement the well-established plate counting, providing useful information about the ratio of comp...romised bacteria in the samples.
Кључне речи:
electrostatic droplet generation / calcium alginate beads / Lactobacillus / viability real-time PCR / propidium monoazideИзвор:
Beneficial Microbes, 2015, 6, 4, 573-581Издавач:
- Wageningen Academic Publishers, Wageningen
Финансирање / пројекти:
- Republic of Slovenia [BI-SL/10-11-035]
- Bilateral Project of the Republic of Serbia
- Slovenian Ministry of Higher Education, Science and Technology
- European Social FundEuropean Social Fund (ESF)
DOI: 10.3920/BM2014.0095
ISSN: 1876-2883
PubMed: 25653154
WoS: 000367160600020
Scopus: 2-s2.0-84931402569
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Oketić, K. AU - Bogović-Matijasić, B. AU - Obermajer, T. AU - Radulović, Zorica AU - Lević, Steva AU - Mirković, Nemanja AU - Nedović, Viktor PY - 2015 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3763 AB - The aim of the study was to evaluate real-time PCR coupled with propidium monoazide (PMA) treatment for enumeration of microencapsulated probiotic lactobacilli microencapsulated in calcium alginate beads. Lactobacillus gasseri K7 (CCM 7710) and Lactobacillus delbrueckii subsp. bulgaricus (CCM 7712) were analysed by plate counting and PMA real-time PCR during storage at 4 degrees C for 90 days. PMA was effective in preventing PCR amplification of the target sequences of DNA released from heat-compromised bacteria. The values obtained by real-time PCR of non-treated samples were in general higher than those obtained by real-time PCR of PMA-treated samples or by plate counting, indicating the presence of sub-lethally injured cells. This study shows that plate count could not be completely replaced by culture independent method PMA real-time PCR for enumeration of probiotics, but may rather complement the well-established plate counting, providing useful information about the ratio of compromised bacteria in the samples. PB - Wageningen Academic Publishers, Wageningen T2 - Beneficial Microbes T1 - Evaluation of propidium monoazide real-time PCR for enumeration of probiotic lactobacilli microencapsulated in calcium alginate beads EP - 581 IS - 4 SP - 573 VL - 6 DO - 10.3920/BM2014.0095 ER -
@article{ author = "Oketić, K. and Bogović-Matijasić, B. and Obermajer, T. and Radulović, Zorica and Lević, Steva and Mirković, Nemanja and Nedović, Viktor", year = "2015", abstract = "The aim of the study was to evaluate real-time PCR coupled with propidium monoazide (PMA) treatment for enumeration of microencapsulated probiotic lactobacilli microencapsulated in calcium alginate beads. Lactobacillus gasseri K7 (CCM 7710) and Lactobacillus delbrueckii subsp. bulgaricus (CCM 7712) were analysed by plate counting and PMA real-time PCR during storage at 4 degrees C for 90 days. PMA was effective in preventing PCR amplification of the target sequences of DNA released from heat-compromised bacteria. The values obtained by real-time PCR of non-treated samples were in general higher than those obtained by real-time PCR of PMA-treated samples or by plate counting, indicating the presence of sub-lethally injured cells. This study shows that plate count could not be completely replaced by culture independent method PMA real-time PCR for enumeration of probiotics, but may rather complement the well-established plate counting, providing useful information about the ratio of compromised bacteria in the samples.", publisher = "Wageningen Academic Publishers, Wageningen", journal = "Beneficial Microbes", title = "Evaluation of propidium monoazide real-time PCR for enumeration of probiotic lactobacilli microencapsulated in calcium alginate beads", pages = "581-573", number = "4", volume = "6", doi = "10.3920/BM2014.0095" }
Oketić, K., Bogović-Matijasić, B., Obermajer, T., Radulović, Z., Lević, S., Mirković, N.,& Nedović, V.. (2015). Evaluation of propidium monoazide real-time PCR for enumeration of probiotic lactobacilli microencapsulated in calcium alginate beads. in Beneficial Microbes Wageningen Academic Publishers, Wageningen., 6(4), 573-581. https://doi.org/10.3920/BM2014.0095
Oketić K, Bogović-Matijasić B, Obermajer T, Radulović Z, Lević S, Mirković N, Nedović V. Evaluation of propidium monoazide real-time PCR for enumeration of probiotic lactobacilli microencapsulated in calcium alginate beads. in Beneficial Microbes. 2015;6(4):573-581. doi:10.3920/BM2014.0095 .
Oketić, K., Bogović-Matijasić, B., Obermajer, T., Radulović, Zorica, Lević, Steva, Mirković, Nemanja, Nedović, Viktor, "Evaluation of propidium monoazide real-time PCR for enumeration of probiotic lactobacilli microencapsulated in calcium alginate beads" in Beneficial Microbes, 6, no. 4 (2015):573-581, https://doi.org/10.3920/BM2014.0095 . .