Did the Iceman Know Better? Screening of the Medicinal Properties of the Birch Polypore Medicinal Mushroom, Piptoporus betulinus (Higher Basidiomycetes)
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Vunduk, JovanaKlaus, Anita
Kozarski, Maja
Petrović, Predrag
Zizak, Zeljko
Nikšić, Miomir
van Griensven, Leo
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The birch polypore Piptoporus betulinus was among two mushrooms that were found in the Iceman's bag. Recent studies indicated that P. betulinus was probably used as a religious and medicinal item. In order to examine the medicinal potential of P. betulinus, hot water (HW), partially purified (PP), and alkali extract (HA) were prepared and tested for antioxidant, antimicrobial, cytotoxic, and angiotensin I-converting enzyme (ACE) inhibitory activity. All tested samples exhibited moderate cytotoxic activity, and HW appeared as the most effective (IC50 = 0.8 +/- 0.1 mg/ml for HeLa cells). HA proved to be a good 1,1-diphenyl-2-picrylhydrazyl radical scavenger and exhibited the strongest ferric-reducing power (EC50 = 0.07 +/- 0.3 mg/ml). The same extract (HA) also expressed the strongest ferric-reducing power (EC50 = 0.99 +/- 0.1 mg/ml). Hot alkali extraction contributed significantly to ACE inhibitory activity (EC50 = 0.06 +/- 0.00 mg/ml) and to antimicrobial activity, especially against h...ighly resistant Enterococcus faecalis (minimum inhibitory concentration: 0.156 +/- 0.000 mg/ml; and minimum bactericidal concentration: 1.25 +/- 0.00 mg/ml).
Keywords:
medicinal mushrooms / angiotensin I-converting enzyme (ACE) / antioxidant activity / cytotoxic activity / Enterococcus faecalis / Iceman / Piptoporus betulinusSource:
International Journal of Medicinal Mushrooms, 2015, 17, 12, 1113-1125Publisher:
- Begell House Inc, Danbury
Funding / projects:
- Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (EU-FP7-316004)
- Develooment and utilization of novel and traditional technologies in production of competitive food products with added valued for national and global market - CREATING WEALTH FROM THE WEALTH OF SERBIA (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46001)
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.1615/IntJMedMushrooms.v17.i12.10
ISSN: 1521-9437
PubMed: 26854098
WoS: 000368923000001
Scopus: 2-s2.0-84955461039
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Institution/Community
Poljoprivredni fakultetTY - JOUR AU - Vunduk, Jovana AU - Klaus, Anita AU - Kozarski, Maja AU - Petrović, Predrag AU - Zizak, Zeljko AU - Nikšić, Miomir AU - van Griensven, Leo PY - 2015 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3717 AB - The birch polypore Piptoporus betulinus was among two mushrooms that were found in the Iceman's bag. Recent studies indicated that P. betulinus was probably used as a religious and medicinal item. In order to examine the medicinal potential of P. betulinus, hot water (HW), partially purified (PP), and alkali extract (HA) were prepared and tested for antioxidant, antimicrobial, cytotoxic, and angiotensin I-converting enzyme (ACE) inhibitory activity. All tested samples exhibited moderate cytotoxic activity, and HW appeared as the most effective (IC50 = 0.8 +/- 0.1 mg/ml for HeLa cells). HA proved to be a good 1,1-diphenyl-2-picrylhydrazyl radical scavenger and exhibited the strongest ferric-reducing power (EC50 = 0.07 +/- 0.3 mg/ml). The same extract (HA) also expressed the strongest ferric-reducing power (EC50 = 0.99 +/- 0.1 mg/ml). Hot alkali extraction contributed significantly to ACE inhibitory activity (EC50 = 0.06 +/- 0.00 mg/ml) and to antimicrobial activity, especially against highly resistant Enterococcus faecalis (minimum inhibitory concentration: 0.156 +/- 0.000 mg/ml; and minimum bactericidal concentration: 1.25 +/- 0.00 mg/ml). PB - Begell House Inc, Danbury T2 - International Journal of Medicinal Mushrooms T1 - Did the Iceman Know Better? Screening of the Medicinal Properties of the Birch Polypore Medicinal Mushroom, Piptoporus betulinus (Higher Basidiomycetes) EP - 1125 IS - 12 SP - 1113 VL - 17 DO - 10.1615/IntJMedMushrooms.v17.i12.10 ER -
@article{ author = "Vunduk, Jovana and Klaus, Anita and Kozarski, Maja and Petrović, Predrag and Zizak, Zeljko and Nikšić, Miomir and van Griensven, Leo", year = "2015", abstract = "The birch polypore Piptoporus betulinus was among two mushrooms that were found in the Iceman's bag. Recent studies indicated that P. betulinus was probably used as a religious and medicinal item. In order to examine the medicinal potential of P. betulinus, hot water (HW), partially purified (PP), and alkali extract (HA) were prepared and tested for antioxidant, antimicrobial, cytotoxic, and angiotensin I-converting enzyme (ACE) inhibitory activity. All tested samples exhibited moderate cytotoxic activity, and HW appeared as the most effective (IC50 = 0.8 +/- 0.1 mg/ml for HeLa cells). HA proved to be a good 1,1-diphenyl-2-picrylhydrazyl radical scavenger and exhibited the strongest ferric-reducing power (EC50 = 0.07 +/- 0.3 mg/ml). The same extract (HA) also expressed the strongest ferric-reducing power (EC50 = 0.99 +/- 0.1 mg/ml). Hot alkali extraction contributed significantly to ACE inhibitory activity (EC50 = 0.06 +/- 0.00 mg/ml) and to antimicrobial activity, especially against highly resistant Enterococcus faecalis (minimum inhibitory concentration: 0.156 +/- 0.000 mg/ml; and minimum bactericidal concentration: 1.25 +/- 0.00 mg/ml).", publisher = "Begell House Inc, Danbury", journal = "International Journal of Medicinal Mushrooms", title = "Did the Iceman Know Better? Screening of the Medicinal Properties of the Birch Polypore Medicinal Mushroom, Piptoporus betulinus (Higher Basidiomycetes)", pages = "1125-1113", number = "12", volume = "17", doi = "10.1615/IntJMedMushrooms.v17.i12.10" }
Vunduk, J., Klaus, A., Kozarski, M., Petrović, P., Zizak, Z., Nikšić, M.,& van Griensven, L.. (2015). Did the Iceman Know Better? Screening of the Medicinal Properties of the Birch Polypore Medicinal Mushroom, Piptoporus betulinus (Higher Basidiomycetes). in International Journal of Medicinal Mushrooms Begell House Inc, Danbury., 17(12), 1113-1125. https://doi.org/10.1615/IntJMedMushrooms.v17.i12.10
Vunduk J, Klaus A, Kozarski M, Petrović P, Zizak Z, Nikšić M, van Griensven L. Did the Iceman Know Better? Screening of the Medicinal Properties of the Birch Polypore Medicinal Mushroom, Piptoporus betulinus (Higher Basidiomycetes). in International Journal of Medicinal Mushrooms. 2015;17(12):1113-1125. doi:10.1615/IntJMedMushrooms.v17.i12.10 .
Vunduk, Jovana, Klaus, Anita, Kozarski, Maja, Petrović, Predrag, Zizak, Zeljko, Nikšić, Miomir, van Griensven, Leo, "Did the Iceman Know Better? Screening of the Medicinal Properties of the Birch Polypore Medicinal Mushroom, Piptoporus betulinus (Higher Basidiomycetes)" in International Journal of Medicinal Mushrooms, 17, no. 12 (2015):1113-1125, https://doi.org/10.1615/IntJMedMushrooms.v17.i12.10 . .