Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, "A fabrum" and A. pusense. This was further supported by average nucleotide identity values ( lt 92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation ...from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)).
Keywords:
Agrobacterium / Crown gall / 16S rDNA / MLSA / ANI / Taxonomy
Source:
Systematic and Applied Microbiology, 2015, 38, 6, 373-378
TY - JOUR
AU - Kuzmanović, Nemanja
AU - Pulawska, Joanna
AU - Prokić, Andjelka
AU - Ivanović, Milan
AU - Zlatković, Nevena
AU - Jones, Jeffrey B.
AU - Obradović, Aleksa
PY - 2015
UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3716
AB - Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, "A fabrum" and A. pusense. This was further supported by average nucleotide identity values ( lt 92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)).
PB - Elsevier Gmbh, Munich
T2 - Systematic and Applied Microbiology
T1 - Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum
EP - 378
IS - 6
SP - 373
VL - 38
DO - 10.1016/j.syapm.2015.06.001
ER -
@article{
author = "Kuzmanović, Nemanja and Pulawska, Joanna and Prokić, Andjelka and Ivanović, Milan and Zlatković, Nevena and Jones, Jeffrey B. and Obradović, Aleksa",
year = "2015",
abstract = "Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, "A fabrum" and A. pusense. This was further supported by average nucleotide identity values ( lt 92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)).",
publisher = "Elsevier Gmbh, Munich",
journal = "Systematic and Applied Microbiology",
title = "Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum",
pages = "378-373",
number = "6",
volume = "38",
doi = "10.1016/j.syapm.2015.06.001"
}
Kuzmanović, N., Pulawska, J., Prokić, A., Ivanović, M., Zlatković, N., Jones, J. B.,& Obradović, A.. (2015). Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum. in Systematic and Applied Microbiology
Elsevier Gmbh, Munich., 38(6), 373-378.
https://doi.org/10.1016/j.syapm.2015.06.001
Kuzmanović N, Pulawska J, Prokić A, Ivanović M, Zlatković N, Jones JB, Obradović A. Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum. in Systematic and Applied Microbiology. 2015;38(6):373-378.
doi:10.1016/j.syapm.2015.06.001 .
Kuzmanović, Nemanja, Pulawska, Joanna, Prokić, Andjelka, Ivanović, Milan, Zlatković, Nevena, Jones, Jeffrey B., Obradović, Aleksa, "Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum" in Systematic and Applied Microbiology, 38, no. 6 (2015):373-378,
https://doi.org/10.1016/j.syapm.2015.06.001 . .
