Structural changes of Ca-alginate beads caused by immobilized yeast cell growth
Само за регистроване кориснике
2015
Аутори
Pajić-Lijaković, IvanaLević, Steva
Hadnadjev, Miroslav
Dajić-Stevanović, Zora
Radošević, Radenko
Nedović, Viktor
Bugarski, Branko
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Structural changes of Ca-alginate bead due to yeast cell loading and the impact on their mechanical properties were studied based on experimental data of cell number density per beads, cell rearrangement within the bead surface and core regions, bead average volume and density, bead storage and loss moduli. Structural changes could be explained by (1) mechanical and electrostatic cell-matrix interactions and (2) chemical interactions of the matrix with the components of the nutrition medium which include: wash out of Ca2+ ions by presence of non-gelling ions and by chelating compounds such as phosphate. According to obtained results, it was estimated that: (1) cell-matrix electrostatic interactions within the hydrogel matrix at t=0 induced the bead weakening, (2) wash out of Ca2+ ions by presence of non-gelling ions and by chelating compounds such as phosphate led to the bead weakening (regime 1, t is an element of (left perpendicular0, 2 daysright perpendicular), (3) the bead stiffnes...s remained constant (regime 2, t is an element of (left perpendicular2, 4 daysright perpendicular)) and (4) the bead reinforcement was caused by cell clusters rapid increase and their inter connections within the bead surface region (regime 3, t is an element of (left perpendicular4, 5 daysright perpendicular)). Regulation of the matrix resistance stress and the rate of its change could lead to decrease of the micro-environmental restriction effects and the whole process optimization.
Кључне речи:
Ca-alginate bead / Yeast / Immobilized cells / Modelling / Rheology / Cell growthИзвор:
Biochemical Engineering Journal, 2015, 103, 32-38Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (EU-FP7-316004)
- Развој и примена нових и традиционалних технологија у производњи конкурентних прехрамбених производа са додатом вредношћу за европско и светско тржиште - Створимо богатство из богатства Србије (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46001)
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.1016/j.bej.2015.06.016
ISSN: 1369-703X
WoS: 000362137400004
Scopus: 2-s2.0-84936866199
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Pajić-Lijaković, Ivana AU - Lević, Steva AU - Hadnadjev, Miroslav AU - Dajić-Stevanović, Zora AU - Radošević, Radenko AU - Nedović, Viktor AU - Bugarski, Branko PY - 2015 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3701 AB - Structural changes of Ca-alginate bead due to yeast cell loading and the impact on their mechanical properties were studied based on experimental data of cell number density per beads, cell rearrangement within the bead surface and core regions, bead average volume and density, bead storage and loss moduli. Structural changes could be explained by (1) mechanical and electrostatic cell-matrix interactions and (2) chemical interactions of the matrix with the components of the nutrition medium which include: wash out of Ca2+ ions by presence of non-gelling ions and by chelating compounds such as phosphate. According to obtained results, it was estimated that: (1) cell-matrix electrostatic interactions within the hydrogel matrix at t=0 induced the bead weakening, (2) wash out of Ca2+ ions by presence of non-gelling ions and by chelating compounds such as phosphate led to the bead weakening (regime 1, t is an element of (left perpendicular0, 2 daysright perpendicular), (3) the bead stiffness remained constant (regime 2, t is an element of (left perpendicular2, 4 daysright perpendicular)) and (4) the bead reinforcement was caused by cell clusters rapid increase and their inter connections within the bead surface region (regime 3, t is an element of (left perpendicular4, 5 daysright perpendicular)). Regulation of the matrix resistance stress and the rate of its change could lead to decrease of the micro-environmental restriction effects and the whole process optimization. PB - Elsevier Science Bv, Amsterdam T2 - Biochemical Engineering Journal T1 - Structural changes of Ca-alginate beads caused by immobilized yeast cell growth EP - 38 SP - 32 VL - 103 DO - 10.1016/j.bej.2015.06.016 ER -
@article{ author = "Pajić-Lijaković, Ivana and Lević, Steva and Hadnadjev, Miroslav and Dajić-Stevanović, Zora and Radošević, Radenko and Nedović, Viktor and Bugarski, Branko", year = "2015", abstract = "Structural changes of Ca-alginate bead due to yeast cell loading and the impact on their mechanical properties were studied based on experimental data of cell number density per beads, cell rearrangement within the bead surface and core regions, bead average volume and density, bead storage and loss moduli. Structural changes could be explained by (1) mechanical and electrostatic cell-matrix interactions and (2) chemical interactions of the matrix with the components of the nutrition medium which include: wash out of Ca2+ ions by presence of non-gelling ions and by chelating compounds such as phosphate. According to obtained results, it was estimated that: (1) cell-matrix electrostatic interactions within the hydrogel matrix at t=0 induced the bead weakening, (2) wash out of Ca2+ ions by presence of non-gelling ions and by chelating compounds such as phosphate led to the bead weakening (regime 1, t is an element of (left perpendicular0, 2 daysright perpendicular), (3) the bead stiffness remained constant (regime 2, t is an element of (left perpendicular2, 4 daysright perpendicular)) and (4) the bead reinforcement was caused by cell clusters rapid increase and their inter connections within the bead surface region (regime 3, t is an element of (left perpendicular4, 5 daysright perpendicular)). Regulation of the matrix resistance stress and the rate of its change could lead to decrease of the micro-environmental restriction effects and the whole process optimization.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Biochemical Engineering Journal", title = "Structural changes of Ca-alginate beads caused by immobilized yeast cell growth", pages = "38-32", volume = "103", doi = "10.1016/j.bej.2015.06.016" }
Pajić-Lijaković, I., Lević, S., Hadnadjev, M., Dajić-Stevanović, Z., Radošević, R., Nedović, V.,& Bugarski, B.. (2015). Structural changes of Ca-alginate beads caused by immobilized yeast cell growth. in Biochemical Engineering Journal Elsevier Science Bv, Amsterdam., 103, 32-38. https://doi.org/10.1016/j.bej.2015.06.016
Pajić-Lijaković I, Lević S, Hadnadjev M, Dajić-Stevanović Z, Radošević R, Nedović V, Bugarski B. Structural changes of Ca-alginate beads caused by immobilized yeast cell growth. in Biochemical Engineering Journal. 2015;103:32-38. doi:10.1016/j.bej.2015.06.016 .
Pajić-Lijaković, Ivana, Lević, Steva, Hadnadjev, Miroslav, Dajić-Stevanović, Zora, Radošević, Radenko, Nedović, Viktor, Bugarski, Branko, "Structural changes of Ca-alginate beads caused by immobilized yeast cell growth" in Biochemical Engineering Journal, 103 (2015):32-38, https://doi.org/10.1016/j.bej.2015.06.016 . .