Detection and molecular characterization of pepper mild mottle virus in Serbia

2015
Authors
Milošević, Dragana
Stanković, Ivana

Bulajić, Aleksandra

Ignjatov, Maja

Nikolić, Zorica

Petrović, Gordana R.
Krstić, Branka
Article (Published version)
Metadata
Show full item recordAbstract
During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L-3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which b...elong to pathotypes P-1,P-2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.
Keywords:
DAS-ELISA / phylogenic analysis / pepper / RT-PCR / sequencing / virusesSource:
Genetika, 2015, 47, 2, 651-663Publisher:
- Društvo genetičara Srbije, Beograd
Funding / projects:
- Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (EU-316004)
- Development of vegetable cultivars and hybrids intended for outdoor and indoor production (RS-31030)
- Agrobiodiversity and land-use change in Serbia: an integrated biodiversity assessment of key functional groups of arthropods and plant pathogens (RS-43001)
DOI: 10.2298/GENSR1502651M
ISSN: 0534-0012
WoS: 000363055800025
Scopus: 2-s2.0-84945919526
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Institution/Community
Poljoprivredni fakultetTY - JOUR AU - Milošević, Dragana AU - Stanković, Ivana AU - Bulajić, Aleksandra AU - Ignjatov, Maja AU - Nikolić, Zorica AU - Petrović, Gordana R. AU - Krstić, Branka PY - 2015 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3684 AB - During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L-3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P-1,P-2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country. PB - Društvo genetičara Srbije, Beograd T2 - Genetika T1 - Detection and molecular characterization of pepper mild mottle virus in Serbia EP - 663 IS - 2 SP - 651 VL - 47 DO - 10.2298/GENSR1502651M ER -
@article{ author = "Milošević, Dragana and Stanković, Ivana and Bulajić, Aleksandra and Ignjatov, Maja and Nikolić, Zorica and Petrović, Gordana R. and Krstić, Branka", year = "2015", abstract = "During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L-3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P-1,P-2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika", title = "Detection and molecular characterization of pepper mild mottle virus in Serbia", pages = "663-651", number = "2", volume = "47", doi = "10.2298/GENSR1502651M" }
Milošević, D., Stanković, I., Bulajić, A., Ignjatov, M., Nikolić, Z., Petrović, G. R.,& Krstić, B.. (2015). Detection and molecular characterization of pepper mild mottle virus in Serbia. in Genetika Društvo genetičara Srbije, Beograd., 47(2), 651-663. https://doi.org/10.2298/GENSR1502651M
Milošević D, Stanković I, Bulajić A, Ignjatov M, Nikolić Z, Petrović GR, Krstić B. Detection and molecular characterization of pepper mild mottle virus in Serbia. in Genetika. 2015;47(2):651-663. doi:10.2298/GENSR1502651M .
Milošević, Dragana, Stanković, Ivana, Bulajić, Aleksandra, Ignjatov, Maja, Nikolić, Zorica, Petrović, Gordana R., Krstić, Branka, "Detection and molecular characterization of pepper mild mottle virus in Serbia" in Genetika, 47, no. 2 (2015):651-663, https://doi.org/10.2298/GENSR1502651M . .