The effect of temperature on pollen germination and pollen tube growth of sour cherry cultivars

Abstract

The study was carried out to determine the effect of three different temperatures (5, 15 and 25°C) on in vitro pollen germination and pollen tube growth of five sour cherry cultivars: 'Heimanns Konservenweichsel', 'Kelleriis 14', 'Oblačinska', 'Rexelle' and 'Šumadinka'. Pollen germination a % agar % Temperature significantly affected pollen germination. High germination rates (50-70%) were obtained at both 15°C and 25°C. Satisfactory germination rates (42-51%) were also obtained at 5°C in some cultivars ('Rexelle', 'Šumadinka' and 'Heimanns Konservenweichsel'). The influence of temperature on the pollen tube growth was more prominent. The length of pollen tubes was three to six times higher at 15°C and 25°C in comparison with 5°C. This has led to the conclusion that the temperature of 5°C, although it could be adequate for pollen germination, is not high enough for optimal pollen tube growth. was determined by germinating pollen grains in culture medium containing 0.7agar-and 15 sucrose.

Ispitivan je uticaj tri različite temperature (5, 15 i 25°C) na klijavost polena i rast polenovih cevčica in vitro kod pet sorti višnje: Hajmanova konzervna, Keleris 14, Oblačinska, Reksele i Šumadinka. Klijavost polena je određivana naklijavanjem polenovih zrna na hranljive podloge koje su se sastojale od 0,7% agar-agara i 15% saharoze. Temperatura je ispoljila značajan uticaj na klijavost polena. Visoka klijavost polena (50-70%) dobijena je na temperaturama od 15°C i 25°C. Zadovoljavajuća klijavost polena (42-51%) takođe je dobijena na temperaturi od 5°C kod nekih sorti (Reksele, Šumadinka i Hajmanova konzervna). Uticaj temperature je bio znatno više izražen na rast polenovih cevčica u odnosu na klijavost polena. Dužina polenovih cevčica je bila tri do šest puta veća na temperaturama od 15°C i 25°C u poređenju sa temperaturom od 5°C. Na osnovu dobijenih rezultata se može zaključiti da temperatura od 5°C, iako može biti dovoljna za klijavost polena, nije dovoljno visoka za optimalan rast polenovih cevčica.