C. apii is one of the most damaging plant pathogenic fungi of celery. During the 2012, samples of celery with typical symptoms of leaf blight were collected on the territory of Veliko Grdaište and their identification is performed using morphological and molecular methods. The pathogenicity test was conducted on healthy plants and all tested isolates caused typical symptoms after inoculation. Conidiophores arising from the stromata formed dense fascicles or single, straight to slightly curved, brown. Conidia are solitary, hyaline and septate (5-13 septa), cylindrical to obclavate, or straight to slightly curved, obtuse at the apex. Truncated and thickened at the base. Fragments of the expected length for the internal transcribed spacer (ITS1/ITS4), calmodulin (CAL-228F/CAL2Rd) and histone (CYLH3F/ CYLH3R) gene were amplified and the obtained sequences showed 100% identity with the C. apii sequences deposited in GenBank.
C. apii predstavlja jednu od najštetnijih fitopatogenih gljiva celera. Tokom 2012. god na teritoriji Velikog Gradišta su prikupljeni uzorci celera sa karakterističnim simptomima pegavosti lista i njihova identifikacija je izvršena pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen na zdravim biljkama i svi ispitivani izolati prouzrokuju karakteristične simptome nakon inokulacije. Konidiofore iz stroma formiraju zbijene fascikule ili su pojedinačne, prave do blago zakrivljene, smeđe boje. Konidije su pojedinačne, hijalinske i septirane (5-13 septi), cilindrične do blago sužene na jednoj strani ili prave do blago zakrivljene i zatupaste na vrhu. U osnovi su zasečene, skoro kvadratnog oblika i stanjene. Amplifikovani su fragmenti očekivanih dužina za internal transcribed spacer (ITS1/ITS4), kalmodulin (CAL-228F/CAL2Rd) i histon (CYLH3F/CYLH3R) genima i dobijene sekvence su pokazale 100% identitet sa sekvencama C. apii u bazi gena.
Keywords:
Cercospora apii / Apium graveolens var. dulce / identification / Cercospora apii / Apium graveolens var. dulce / identifikacija
Source:
Zaštita bilja, 2014, 65, 2, 77-84
Publisher:
Institut za zaštitu bilja i životnu sredinu, Beograd
TY - JOUR
AU - Milosavljević, Anja
AU - Pfaf-Dolovac, Erika
AU - Mitrović, Milana
AU - Jović, Jelena
AU - Toševski, Ivo
AU - Duduk, Nataša
AU - Trkulja, Nenad
PY - 2014
UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3621
AB - C. apii is one of the most damaging plant pathogenic fungi of celery. During the 2012, samples of celery with typical symptoms of leaf blight were collected on the territory of Veliko Grdaište and their identification is performed using morphological and molecular methods. The pathogenicity test was conducted on healthy plants and all tested isolates caused typical symptoms after inoculation. Conidiophores arising from the stromata formed dense fascicles or single, straight to slightly curved, brown. Conidia are solitary, hyaline and septate (5-13 septa), cylindrical to obclavate, or straight to slightly curved, obtuse at the apex. Truncated and thickened at the base. Fragments of the expected length for the internal transcribed spacer (ITS1/ITS4), calmodulin (CAL-228F/CAL2Rd) and histone (CYLH3F/ CYLH3R) gene were amplified and the obtained sequences showed 100% identity with the C. apii sequences deposited in GenBank.
AB - C. apii predstavlja jednu od najštetnijih fitopatogenih gljiva celera. Tokom 2012. god na teritoriji Velikog Gradišta su prikupljeni uzorci celera sa karakterističnim simptomima pegavosti lista i njihova identifikacija je izvršena pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen na zdravim biljkama i svi ispitivani izolati prouzrokuju karakteristične simptome nakon inokulacije. Konidiofore iz stroma formiraju zbijene fascikule ili su pojedinačne, prave do blago zakrivljene, smeđe boje. Konidije su pojedinačne, hijalinske i septirane (5-13 septi), cilindrične do blago sužene na jednoj strani ili prave do blago zakrivljene i zatupaste na vrhu. U osnovi su zasečene, skoro kvadratnog oblika i stanjene. Amplifikovani su fragmenti očekivanih dužina za internal transcribed spacer (ITS1/ITS4), kalmodulin (CAL-228F/CAL2Rd) i histon (CYLH3F/CYLH3R) genima i dobijene sekvence su pokazale 100% identitet sa sekvencama C. apii u bazi gena.
PB - Institut za zaštitu bilja i životnu sredinu, Beograd
T2 - Zaštita bilja
T1 - Morphological and molecular identification of Cercospora apii on celery in Serbia
T1 - Morfološka i molekularna identifikacija Cercospora apii na celeru u Srbiji
EP - 84
IS - 2
SP - 77
VL - 65
DO - 10.5937/zasbilj1402077M
ER -
@article{
author = "Milosavljević, Anja and Pfaf-Dolovac, Erika and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Duduk, Nataša and Trkulja, Nenad",
year = "2014",
abstract = "C. apii is one of the most damaging plant pathogenic fungi of celery. During the 2012, samples of celery with typical symptoms of leaf blight were collected on the territory of Veliko Grdaište and their identification is performed using morphological and molecular methods. The pathogenicity test was conducted on healthy plants and all tested isolates caused typical symptoms after inoculation. Conidiophores arising from the stromata formed dense fascicles or single, straight to slightly curved, brown. Conidia are solitary, hyaline and septate (5-13 septa), cylindrical to obclavate, or straight to slightly curved, obtuse at the apex. Truncated and thickened at the base. Fragments of the expected length for the internal transcribed spacer (ITS1/ITS4), calmodulin (CAL-228F/CAL2Rd) and histone (CYLH3F/ CYLH3R) gene were amplified and the obtained sequences showed 100% identity with the C. apii sequences deposited in GenBank., C. apii predstavlja jednu od najštetnijih fitopatogenih gljiva celera. Tokom 2012. god na teritoriji Velikog Gradišta su prikupljeni uzorci celera sa karakterističnim simptomima pegavosti lista i njihova identifikacija je izvršena pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen na zdravim biljkama i svi ispitivani izolati prouzrokuju karakteristične simptome nakon inokulacije. Konidiofore iz stroma formiraju zbijene fascikule ili su pojedinačne, prave do blago zakrivljene, smeđe boje. Konidije su pojedinačne, hijalinske i septirane (5-13 septi), cilindrične do blago sužene na jednoj strani ili prave do blago zakrivljene i zatupaste na vrhu. U osnovi su zasečene, skoro kvadratnog oblika i stanjene. Amplifikovani su fragmenti očekivanih dužina za internal transcribed spacer (ITS1/ITS4), kalmodulin (CAL-228F/CAL2Rd) i histon (CYLH3F/CYLH3R) genima i dobijene sekvence su pokazale 100% identitet sa sekvencama C. apii u bazi gena.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Morphological and molecular identification of Cercospora apii on celery in Serbia, Morfološka i molekularna identifikacija Cercospora apii na celeru u Srbiji",
pages = "84-77",
number = "2",
volume = "65",
doi = "10.5937/zasbilj1402077M"
}
Milosavljević, A., Pfaf-Dolovac, E., Mitrović, M., Jović, J., Toševski, I., Duduk, N.,& Trkulja, N.. (2014). Morphological and molecular identification of Cercospora apii on celery in Serbia. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 65(2), 77-84.
https://doi.org/10.5937/zasbilj1402077M
Milosavljević A, Pfaf-Dolovac E, Mitrović M, Jović J, Toševski I, Duduk N, Trkulja N. Morphological and molecular identification of Cercospora apii on celery in Serbia. in Zaštita bilja. 2014;65(2):77-84.
doi:10.5937/zasbilj1402077M .
Milosavljević, Anja, Pfaf-Dolovac, Erika, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Duduk, Nataša, Trkulja, Nenad, "Morphological and molecular identification of Cercospora apii on celery in Serbia" in Zaštita bilja, 65, no. 2 (2014):77-84,
https://doi.org/10.5937/zasbilj1402077M . .
