Experimental methods applicable in cultivation of medicinal plants

Abstract

This work summarizes the most commonly used methods of research of weed species in medicinal herbs. The choice of method depends on the type of research that we want to conduct. The methods relating to weed herbs are described in detail for the methods of examination of the weeds' seed germination, methods for the determination of reserves of weed plants' seeds in the soil (seed bank), as well as the methods for testing the efficiency of the measures for suppression of weed in remedial plants. Within the methods for the examination of germination, described in detail are the use of greenhouses and the methods for the interruption of seeds' dormancy, with special emphasis on the bases used for the examination of seeds' germination in Petri dishes. If the soil is sampled based on the locality, the procedure for the physical extraction of weed seeds is described, as well as the method of germination of seeds from the sampled soil. Within the methods for testing the efficiency of the measures for suppression of weed in remedial plants, the types of treatment, the way of weed sampling, as well as the examination of the treatment efficiently are described.

U radu su istaknute najčešće korišćene metode istraživanja korovskih vrsta u lekovitom bilju. Odabir metoda zavisi od vrste istraživanja koja se planira.Metode koje se odnose na korovske biljne vrste, detaljno su opisane za ispitivanje klijavosti semena korova, metode za utvrđivanje rezervi semena korovskih biljaka u zemljištu, kao i metode za testiranja efikasnosti mera za suzbijanje korova u lekovitom bilju. U okviru metoda za ispitivanje klijavosti, detaljno je opisana primena klijališta i metode za prekidanje mirovanja semena, sa posebnim osvrtom na podloge koje se koriste prilikom ispitivanja klijavosti semena u Petri šoljama. Ukoliko se uzorkuje zemljište sa određenog lokaliteta, opisan je postupak fizičke ekstrakcije semena korova, kao i metod naklijavanja semena iz uzoraka zemljišta. U okviru metoda za testiranja efikasnosti mera za suzbijanje korova u lekovitom bilju, opisani su vrste tretmana, na koji način se vrši uzorkovanje korova, kao i ispitivanje efikasnosti tretmana. Cilj ovog rada je prikaz određenih metoda koje se mogu koristiti za istraživanja korova u gajenom lekovitom bilju.