Genetic variability of red clover seedlings in relation to salt stress

Abstract

Red clover is highly salt-sensitive plant, especially during germination and early seedling growth stages. The aim of this investigation was to estimate the effects of different saline conditions (0, 50, 100, 150, and 200mM NaCl) on germination and early seedling growth in four red clover varieties (Kolubara, K-32, K-17 and K-39). Germination test was conducted in the laboratory conditions using sterile plastic vessels on filter paper moistened with 10ml of the appropriate salt test solutions. It was observed that the germination energy (GE), germination (G), percentage of dead or infected seeds (DIS), normal seedlings (NS), root length (RL), shoot length (ShL), fresh weight (FW) and dry weight of seedling (DW) and seedling vigor index (SVI) were significantly decreased with increasing concentrations of NaCl in the growing medium. The tested varieties of red clover showed different NaCl tolerance at the seedling stage. Generally, studied red clover varieties are very sensitive to salt, especially K-32 which has the lowest values for GE, G, NS and SVI and highest for DIS. Variety K-17 proved to be a variety that the best tolerates conditions of salt stress because the values for GE, G, NS, RL and SVI were highest. Testing of varieties of red clover in the early seedling growth at different concentrations of NaCl in the growing medium could be helpful in the identification and selection of varieties for cultivation on saline soils.

Crvena detelina je vrlo osetljiva biljka na veću koncentarciju soli, posebno tokom klijanja i rane faze porasta klijanaca. Cilj ovog istraživanja bio je da se proceni uticaj različite zaslanjenosti (0, 50, 100, 150 i 200mM NaCl) na klijanje i rani porast klijanaca četiri sorte crvene deteline (Kolubara, K-32, K-17 i K-39). Test klijavosti sproveden je u laboratorijskim uslovima u sterilnim plastičnim posudama na filter papiru natopljenom sa 10 ml odgovarajuće koncentracije soli. Uočeno je da energija klijanja (EK), klijavost (K), neklijala i bolesna semena (NB), normalni klijanci (NK), dužina korena (DK), dužina hipokotila (DH), sveža (SvMK) i suva masa klijanaca (SuMK) i vigor indeks klijanaca (VIK) se značajno smanjuju sa povećanjem koncentracije NaCl u podlogama za naklijavanje. Ispitivane sorte imale su različitu toleranciju na soni stres u fazi klijanaca. Generalno, proučavane sorte su veoma osetljive na veću koncentraciju soli, posebno K-32 koja je imala najniže vrednosti za EK, K, NK i VIK, kao i najveći broj NB. Sorta K-17 se pokazala kao sorta koja najbolje toleriše soni stres jer su vrednosti za EK, K, NK, DK i VIK bile najviše. Testiranje sorti crvene deteline u ranom porastu klijanaca na podlogama za naklijavanje sa različitom koncentracijom NaCl može pomoći u indentifikaciji i izboru sorti za gajenje na zaslanjenim zemljištima.