Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential
Само за регистроване кориснике
2014
Аутори
Gašić, K.Ivanović, Milan
Kuzmanović, Nemanja
Prokić, Andjelka
Obradović, Aleksa
Šević, Milan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.
Кључне речи:
bacteriophages / host range / fire blight / biological controlИзвор:
Acta Horticulturae, 2014, 1056, 123-126Издавач:
- International Society for Horticultural Science
Финансирање / пројекти:
DOI: 10.17660/ActaHortic.2014.1056.17
ISSN: 0567-7572
WoS: 000357733400017
Scopus: 2-s2.0-84912099519
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Gašić, K. AU - Ivanović, Milan AU - Kuzmanović, Nemanja AU - Prokić, Andjelka AU - Obradović, Aleksa AU - Šević, Milan PY - 2014 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3510 AB - In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation. PB - International Society for Horticultural Science T2 - Acta Horticulturae T1 - Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential EP - 126 SP - 123 VL - 1056 DO - 10.17660/ActaHortic.2014.1056.17 ER -
@article{ author = "Gašić, K. and Ivanović, Milan and Kuzmanović, Nemanja and Prokić, Andjelka and Obradović, Aleksa and Šević, Milan", year = "2014", abstract = "In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.", publisher = "International Society for Horticultural Science", journal = "Acta Horticulturae", title = "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential", pages = "126-123", volume = "1056", doi = "10.17660/ActaHortic.2014.1056.17" }
Gašić, K., Ivanović, M., Kuzmanović, N., Prokić, A., Obradović, A.,& Šević, M.. (2014). Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in Acta Horticulturae International Society for Horticultural Science., 1056, 123-126. https://doi.org/10.17660/ActaHortic.2014.1056.17
Gašić K, Ivanović M, Kuzmanović N, Prokić A, Obradović A, Šević M. Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in Acta Horticulturae. 2014;1056:123-126. doi:10.17660/ActaHortic.2014.1056.17 .
Gašić, K., Ivanović, Milan, Kuzmanović, Nemanja, Prokić, Andjelka, Obradović, Aleksa, Šević, Milan, "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential" in Acta Horticulturae, 1056 (2014):123-126, https://doi.org/10.17660/ActaHortic.2014.1056.17 . .