Profiling antioxidant activity of two primocane fruiting red raspberry cultivars (Autumn bliss and Polka)
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2013
Authors
Dragišić-Maksimović, Jelena J.
Milivojević, Jasminka

Poledica, Milena
Nikolić, Mihailo D.
Maksimović, Vuk

Article (Published version)

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Fruit extracts of two raspberry (Rubus idaeus L) cultivars (Autumn Bliss and Polka) were analysed for total phenolic (TPC) and anthocyanin (TACY) content. Correlation of TPC with total antioxidant capacity (TAC) showed higher free-radical scavenging properties of Autumn Bliss (r(2) = 0.9999) compared to Polka (r(2) = 0.8972). Correlation coefficient between TACY and TAC were higher in Autumn Bliss (r(2) = 0.9939) compared to Polka fruits (r(2) = 0.8419). Although total protein concentrations were similar in both cultivars (similar to 0.35 mg mL(-1)), activities of peroxidases and polyphenol oxidases were much higher in Polka, which were confirmed with isoelectric focusing in cationic (pl 9.3) and anionic (pl 3.6) range. HPLC detection showed that among detected flavonoids (catechin, epicatechin, epicatechin gallate, rutin, myricetin, resveratrol, quercetin and kaempferol) epicatechin appears to be the most abundant compound. Chlorogenic, caffeic and p-coumaric acid were also detected. ...The results indicate that the changes in enzymes activities related to content of substrates play an important role in nutrient quality definition of raspberry fruits.
Keywords:
Antioxidative capacity / Flavonoids / Food analysis / Food composition / HPLC / Isoelectric focusing / Peroxidase / Phenolic acids / Polyphenol oxidase / Primocane raspberries / Rubus idaeus L.Source:
Journal of Food Composition and Analysis, 2013, 31, 2, 173-179Publisher:
- Academic Press Inc Elsevier Science, San Diego
Funding / projects:
- The membranes as sites of interaction between the intracellular and apoplastic environments: studies of the bioenergetics and signaling using biophysical and biochemical techniques. (RS-173040)
- Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety (RS-46008)
DOI: 10.1016/j.jfca.2013.05.008
ISSN: 0889-1575
WoS: 000328308100001
Scopus: 2-s2.0-84886314520
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Poljoprivredni fakultetTY - JOUR AU - Dragišić-Maksimović, Jelena J. AU - Milivojević, Jasminka AU - Poledica, Milena AU - Nikolić, Mihailo D. AU - Maksimović, Vuk PY - 2013 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3090 AB - Fruit extracts of two raspberry (Rubus idaeus L) cultivars (Autumn Bliss and Polka) were analysed for total phenolic (TPC) and anthocyanin (TACY) content. Correlation of TPC with total antioxidant capacity (TAC) showed higher free-radical scavenging properties of Autumn Bliss (r(2) = 0.9999) compared to Polka (r(2) = 0.8972). Correlation coefficient between TACY and TAC were higher in Autumn Bliss (r(2) = 0.9939) compared to Polka fruits (r(2) = 0.8419). Although total protein concentrations were similar in both cultivars (similar to 0.35 mg mL(-1)), activities of peroxidases and polyphenol oxidases were much higher in Polka, which were confirmed with isoelectric focusing in cationic (pl 9.3) and anionic (pl 3.6) range. HPLC detection showed that among detected flavonoids (catechin, epicatechin, epicatechin gallate, rutin, myricetin, resveratrol, quercetin and kaempferol) epicatechin appears to be the most abundant compound. Chlorogenic, caffeic and p-coumaric acid were also detected. The results indicate that the changes in enzymes activities related to content of substrates play an important role in nutrient quality definition of raspberry fruits. PB - Academic Press Inc Elsevier Science, San Diego T2 - Journal of Food Composition and Analysis T1 - Profiling antioxidant activity of two primocane fruiting red raspberry cultivars (Autumn bliss and Polka) EP - 179 IS - 2 SP - 173 VL - 31 DO - 10.1016/j.jfca.2013.05.008 ER -
@article{ author = "Dragišić-Maksimović, Jelena J. and Milivojević, Jasminka and Poledica, Milena and Nikolić, Mihailo D. and Maksimović, Vuk", year = "2013", abstract = "Fruit extracts of two raspberry (Rubus idaeus L) cultivars (Autumn Bliss and Polka) were analysed for total phenolic (TPC) and anthocyanin (TACY) content. Correlation of TPC with total antioxidant capacity (TAC) showed higher free-radical scavenging properties of Autumn Bliss (r(2) = 0.9999) compared to Polka (r(2) = 0.8972). Correlation coefficient between TACY and TAC were higher in Autumn Bliss (r(2) = 0.9939) compared to Polka fruits (r(2) = 0.8419). Although total protein concentrations were similar in both cultivars (similar to 0.35 mg mL(-1)), activities of peroxidases and polyphenol oxidases were much higher in Polka, which were confirmed with isoelectric focusing in cationic (pl 9.3) and anionic (pl 3.6) range. HPLC detection showed that among detected flavonoids (catechin, epicatechin, epicatechin gallate, rutin, myricetin, resveratrol, quercetin and kaempferol) epicatechin appears to be the most abundant compound. Chlorogenic, caffeic and p-coumaric acid were also detected. The results indicate that the changes in enzymes activities related to content of substrates play an important role in nutrient quality definition of raspberry fruits.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Journal of Food Composition and Analysis", title = "Profiling antioxidant activity of two primocane fruiting red raspberry cultivars (Autumn bliss and Polka)", pages = "179-173", number = "2", volume = "31", doi = "10.1016/j.jfca.2013.05.008" }
Dragišić-Maksimović, J. J., Milivojević, J., Poledica, M., Nikolić, M. D.,& Maksimović, V.. (2013). Profiling antioxidant activity of two primocane fruiting red raspberry cultivars (Autumn bliss and Polka). in Journal of Food Composition and Analysis Academic Press Inc Elsevier Science, San Diego., 31(2), 173-179. https://doi.org/10.1016/j.jfca.2013.05.008
Dragišić-Maksimović JJ, Milivojević J, Poledica M, Nikolić MD, Maksimović V. Profiling antioxidant activity of two primocane fruiting red raspberry cultivars (Autumn bliss and Polka). in Journal of Food Composition and Analysis. 2013;31(2):173-179. doi:10.1016/j.jfca.2013.05.008 .
Dragišić-Maksimović, Jelena J., Milivojević, Jasminka, Poledica, Milena, Nikolić, Mihailo D., Maksimović, Vuk, "Profiling antioxidant activity of two primocane fruiting red raspberry cultivars (Autumn bliss and Polka)" in Journal of Food Composition and Analysis, 31, no. 2 (2013):173-179, https://doi.org/10.1016/j.jfca.2013.05.008 . .