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dc.creatorWang, S.L.
dc.creatorChen, D.
dc.creatorGuo, G.F.
dc.creatorZhang, T.
dc.creatorJiang, S.S.
dc.creatorShen, X.X.
dc.creatorPerović, D.
dc.creatorProdanović, Slaven
dc.creatorYan, Y.M.
dc.date.accessioned2020-12-17T20:34:30Z
dc.date.available2020-12-17T20:34:30Z
dc.date.issued2012
dc.identifier.issn0133-3720
dc.identifier.urihttp://aspace.agrif.bg.ac.rs/handle/123456789/3038
dc.description.abstractIn this work, 9 novel LMW-GS genes (6 LMW-m and 3 LMW-i type) from 4 diploid and 1 tetraploid Aegilops species were amplified and cloned by allelic-specific PCR. Analysis of the deduced amino acid sequences showed that 7 and 2 LMW-GS had 9 and 7 cysteines, respectively. Four LMW-m type subunits genes had an extra cysteine at the C-terminal III, which could form intermolecular disulphide bonds to extend the chains, and therefore would facilitate to form larger gluten polymers. This suggested that these genes are expected to be used as candidate genes for wheat quality improvement. The correlation between specific N-terminal sequences and a decapeptide deletion in the C-terminal II in LMW-GS encoded by D genome was found. Particularly, if LMW-GS possessed a METRCIPG-N-terminal beginning sequences and a decapeptide (LGQCSFQQPQ) deletion in the C-terminal II, they could be encoded by D genome.en
dc.publisherAkademiai Kiado Zrt, Budapest
dc.relationNational Natural Science Foundation of China (NSFC) [30830072]
dc.relationChinese Ministry of Science and TechnologyMinistry of Science and Technology, China [2009CB118303]
dc.relationNational Key Project for Transgenic Crops of China [2008ZX08002-004]
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31069/RS//
dc.rightsrestrictedAccess
dc.sourceCereal Research Communications
dc.subjectAegilops speciesen
dc.subjectLMW-GS genesen
dc.subjectN-terminal domainen
dc.subjectconserved cysteinesen
dc.titleMolecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Speciesen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage551
dc.citation.issue4
dc.citation.other40(4): 542-551
dc.citation.rankM23
dc.citation.spage542
dc.citation.volume40
dc.identifier.doi10.1556/CRC.40.2012.0006
dc.identifier.scopus2-s2.0-84870582053
dc.identifier.wos000311807200010
dc.type.versionpublishedVersion


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