Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination
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2012
Authors
Šmigić, Nada
Rajković, Andreja

Arneborg, Nils

Siegumfeldt, Henrik

Devlieghere, Frank
Nielsen, Dennis Sandris

Article (Published version)

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Show full item recordAbstract
The aim of this study was to investigate the response of Escherichia coli O157:H7 when exposed to different concentrations of sanitation agent chlorine dioxide (ClO2) by determining intracellular pH (pH(i)). For this purpose, fluorescence ratio imaging microscopy was used together with pH-sensitive, ratiometric green fluorescent protein that was introduced in E. coli O157:H7 cells. Along with pH(i), colony counts were determined during the treatment with ClO2. Results revealed several post-treatment subpopulations with different physiological states, as judged by their pH(i). The fraction of cells with no pH gradient increased, and the colony count decreased as the concentration of ClO2 increased.
Keywords:
Escherichia coli O157:H7 / Chlorine dioxide / Sublethal injury / Intracellular pHSource:
Food Analytical Methods, 2012, 5, 3, 327-331Publisher:
- Springer, New York
Funding / projects:
- European CommissionEuropean CommissionEuropean Commission Joint Research Centre [FOOD-CT-2005-007081]
DOI: 10.1007/s12161-011-9295-0
ISSN: 1936-9751
WoS: 000303466800002
Scopus: 2-s2.0-84860474116
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Poljoprivredni fakultetTY - JOUR AU - Šmigić, Nada AU - Rajković, Andreja AU - Arneborg, Nils AU - Siegumfeldt, Henrik AU - Devlieghere, Frank AU - Nielsen, Dennis Sandris PY - 2012 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3024 AB - The aim of this study was to investigate the response of Escherichia coli O157:H7 when exposed to different concentrations of sanitation agent chlorine dioxide (ClO2) by determining intracellular pH (pH(i)). For this purpose, fluorescence ratio imaging microscopy was used together with pH-sensitive, ratiometric green fluorescent protein that was introduced in E. coli O157:H7 cells. Along with pH(i), colony counts were determined during the treatment with ClO2. Results revealed several post-treatment subpopulations with different physiological states, as judged by their pH(i). The fraction of cells with no pH gradient increased, and the colony count decreased as the concentration of ClO2 increased. PB - Springer, New York T2 - Food Analytical Methods T1 - Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination EP - 331 IS - 3 SP - 327 VL - 5 DO - 10.1007/s12161-011-9295-0 ER -
@article{ author = "Šmigić, Nada and Rajković, Andreja and Arneborg, Nils and Siegumfeldt, Henrik and Devlieghere, Frank and Nielsen, Dennis Sandris", year = "2012", abstract = "The aim of this study was to investigate the response of Escherichia coli O157:H7 when exposed to different concentrations of sanitation agent chlorine dioxide (ClO2) by determining intracellular pH (pH(i)). For this purpose, fluorescence ratio imaging microscopy was used together with pH-sensitive, ratiometric green fluorescent protein that was introduced in E. coli O157:H7 cells. Along with pH(i), colony counts were determined during the treatment with ClO2. Results revealed several post-treatment subpopulations with different physiological states, as judged by their pH(i). The fraction of cells with no pH gradient increased, and the colony count decreased as the concentration of ClO2 increased.", publisher = "Springer, New York", journal = "Food Analytical Methods", title = "Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination", pages = "331-327", number = "3", volume = "5", doi = "10.1007/s12161-011-9295-0" }
Šmigić, N., Rajković, A., Arneborg, N., Siegumfeldt, H., Devlieghere, F.,& Nielsen, D. S.. (2012). Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination. in Food Analytical Methods Springer, New York., 5(3), 327-331. https://doi.org/10.1007/s12161-011-9295-0
Šmigić N, Rajković A, Arneborg N, Siegumfeldt H, Devlieghere F, Nielsen DS. Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination. in Food Analytical Methods. 2012;5(3):327-331. doi:10.1007/s12161-011-9295-0 .
Šmigić, Nada, Rajković, Andreja, Arneborg, Nils, Siegumfeldt, Henrik, Devlieghere, Frank, Nielsen, Dennis Sandris, "Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination" in Food Analytical Methods, 5, no. 3 (2012):327-331, https://doi.org/10.1007/s12161-011-9295-0 . .