The aim of this study was to investigate the response of Escherichia coli O157:H7 when exposed to different concentrations of sanitation agent chlorine dioxide (ClO2) by determining intracellular pH (pH(i)). For this purpose, fluorescence ratio imaging microscopy was used together with pH-sensitive, ratiometric green fluorescent protein that was introduced in E. coli O157:H7 cells. Along with pH(i), colony counts were determined during the treatment with ClO2. Results revealed several post-treatment subpopulations with different physiological states, as judged by their pH(i). The fraction of cells with no pH gradient increased, and the colony count decreased as the concentration of ClO2 increased.
TY - JOUR
AU - Šmigić, Nada
AU - Rajković, Andreja
AU - Arneborg, Nils
AU - Siegumfeldt, Henrik
AU - Devlieghere, Frank
AU - Nielsen, Dennis Sandris
PY - 2012
UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3024
AB - The aim of this study was to investigate the response of Escherichia coli O157:H7 when exposed to different concentrations of sanitation agent chlorine dioxide (ClO2) by determining intracellular pH (pH(i)). For this purpose, fluorescence ratio imaging microscopy was used together with pH-sensitive, ratiometric green fluorescent protein that was introduced in E. coli O157:H7 cells. Along with pH(i), colony counts were determined during the treatment with ClO2. Results revealed several post-treatment subpopulations with different physiological states, as judged by their pH(i). The fraction of cells with no pH gradient increased, and the colony count decreased as the concentration of ClO2 increased.
PB - Springer, New York
T2 - Food Analytical Methods
T1 - Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination
EP - 331
IS - 3
SP - 327
VL - 5
DO - 10.1007/s12161-011-9295-0
ER -
@article{
author = "Šmigić, Nada and Rajković, Andreja and Arneborg, Nils and Siegumfeldt, Henrik and Devlieghere, Frank and Nielsen, Dennis Sandris",
year = "2012",
abstract = "The aim of this study was to investigate the response of Escherichia coli O157:H7 when exposed to different concentrations of sanitation agent chlorine dioxide (ClO2) by determining intracellular pH (pH(i)). For this purpose, fluorescence ratio imaging microscopy was used together with pH-sensitive, ratiometric green fluorescent protein that was introduced in E. coli O157:H7 cells. Along with pH(i), colony counts were determined during the treatment with ClO2. Results revealed several post-treatment subpopulations with different physiological states, as judged by their pH(i). The fraction of cells with no pH gradient increased, and the colony count decreased as the concentration of ClO2 increased.",
publisher = "Springer, New York",
journal = "Food Analytical Methods",
title = "Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination",
pages = "331-327",
number = "3",
volume = "5",
doi = "10.1007/s12161-011-9295-0"
}
Šmigić, N., Rajković, A., Arneborg, N., Siegumfeldt, H., Devlieghere, F.,& Nielsen, D. S.. (2012). Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination. in Food Analytical Methods
Springer, New York., 5(3), 327-331.
https://doi.org/10.1007/s12161-011-9295-0
Šmigić N, Rajković A, Arneborg N, Siegumfeldt H, Devlieghere F, Nielsen DS. Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination. in Food Analytical Methods. 2012;5(3):327-331.
doi:10.1007/s12161-011-9295-0 .
Šmigić, Nada, Rajković, Andreja, Arneborg, Nils, Siegumfeldt, Henrik, Devlieghere, Frank, Nielsen, Dennis Sandris, "Analysis of Intracellular pH in Escherichia coli O157:H7 to Determine the Effect of Chlorine Dioxide Decontamination" in Food Analytical Methods, 5, no. 3 (2012):327-331,
https://doi.org/10.1007/s12161-011-9295-0 . .
