Quantification of viable spray-dried Lactobacillus plantarum TA and 7A after two years of storage by using real-time PCR
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Mirković, Nemanja
Radulović, Zorica
Matijašić, B.J.B.
Petrušić, Milica M.
Petrović, Tanja

Djordjević, Verica
Nedović, Viktor

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Probiotic are "living microorganisms, which upon ingestion in certain numbers, exert health benefits beyond inherent basic nutrition", and it is recommended that probiotic products contain at least 107 live microorganisms per g or per ml. Spray-drying is one of the possibilities to improve the survival of probiotic bacteria against negative environmental effects. The aim of this study was to compare survival of two spray-dried Lactobacillus plantarum TA and Lactobacillus plantarum 7A strains isolated from plant and breast-fed baby's feces. In addition, beside the plate-count technique, the aim was to examine the possibility of using propidium monoazide (PMA) in combination with real-time polymerase chain reaction (real-time PCR) for determination of spray-dried Lb. plantarum strains after two years of storage at 4°C. Both tested strains showed very good ability to survive spray-drying. After two years of storage at 4°C, the number of intact cells, Lb. plantarum TA and Lb. plantarum 7A,... was determined by real-time PCR with PMA, and it was similar to the number of investigated strains obtained by plate count method. The spray-drying was effective in maintaining the viability of Lb. plantarum TA and 7A strains after two years of storage at 4°C. The PMA real-time PCR determination of the viability tested strains could complement the plate count method.
Keywords:
Probiotic bacteria / Propidium monoazide / Real-time PCRSource:
CEFood 2012 - Proceedings of 6th Central European Congress on Food, 2012, 1082-1086Publisher:
- 6th Central European Congress on Food, CEFood 2012
Scopus: 2-s2.0-84926044671
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Poljoprivredni fakultetTY - CONF AU - Mirković, Nemanja AU - Radulović, Zorica AU - Matijašić, B.J.B. AU - Petrušić, Milica M. AU - Petrović, Tanja AU - Djordjević, Verica AU - Nedović, Viktor PY - 2012 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/2776 AB - Probiotic are "living microorganisms, which upon ingestion in certain numbers, exert health benefits beyond inherent basic nutrition", and it is recommended that probiotic products contain at least 107 live microorganisms per g or per ml. Spray-drying is one of the possibilities to improve the survival of probiotic bacteria against negative environmental effects. The aim of this study was to compare survival of two spray-dried Lactobacillus plantarum TA and Lactobacillus plantarum 7A strains isolated from plant and breast-fed baby's feces. In addition, beside the plate-count technique, the aim was to examine the possibility of using propidium monoazide (PMA) in combination with real-time polymerase chain reaction (real-time PCR) for determination of spray-dried Lb. plantarum strains after two years of storage at 4°C. Both tested strains showed very good ability to survive spray-drying. After two years of storage at 4°C, the number of intact cells, Lb. plantarum TA and Lb. plantarum 7A, was determined by real-time PCR with PMA, and it was similar to the number of investigated strains obtained by plate count method. The spray-drying was effective in maintaining the viability of Lb. plantarum TA and 7A strains after two years of storage at 4°C. The PMA real-time PCR determination of the viability tested strains could complement the plate count method. PB - 6th Central European Congress on Food, CEFood 2012 C3 - CEFood 2012 - Proceedings of 6th Central European Congress on Food T1 - Quantification of viable spray-dried Lactobacillus plantarum TA and 7A after two years of storage by using real-time PCR EP - 1086 SP - 1082 UR - https://hdl.handle.net/21.15107/rcub_agrospace_2776 ER -
@conference{ author = "Mirković, Nemanja and Radulović, Zorica and Matijašić, B.J.B. and Petrušić, Milica M. and Petrović, Tanja and Djordjević, Verica and Nedović, Viktor", year = "2012", abstract = "Probiotic are "living microorganisms, which upon ingestion in certain numbers, exert health benefits beyond inherent basic nutrition", and it is recommended that probiotic products contain at least 107 live microorganisms per g or per ml. Spray-drying is one of the possibilities to improve the survival of probiotic bacteria against negative environmental effects. The aim of this study was to compare survival of two spray-dried Lactobacillus plantarum TA and Lactobacillus plantarum 7A strains isolated from plant and breast-fed baby's feces. In addition, beside the plate-count technique, the aim was to examine the possibility of using propidium monoazide (PMA) in combination with real-time polymerase chain reaction (real-time PCR) for determination of spray-dried Lb. plantarum strains after two years of storage at 4°C. Both tested strains showed very good ability to survive spray-drying. After two years of storage at 4°C, the number of intact cells, Lb. plantarum TA and Lb. plantarum 7A, was determined by real-time PCR with PMA, and it was similar to the number of investigated strains obtained by plate count method. The spray-drying was effective in maintaining the viability of Lb. plantarum TA and 7A strains after two years of storage at 4°C. The PMA real-time PCR determination of the viability tested strains could complement the plate count method.", publisher = "6th Central European Congress on Food, CEFood 2012", journal = "CEFood 2012 - Proceedings of 6th Central European Congress on Food", title = "Quantification of viable spray-dried Lactobacillus plantarum TA and 7A after two years of storage by using real-time PCR", pages = "1086-1082", url = "https://hdl.handle.net/21.15107/rcub_agrospace_2776" }
Mirković, N., Radulović, Z., Matijašić, B.J.B., Petrušić, M. M., Petrović, T., Djordjević, V.,& Nedović, V.. (2012). Quantification of viable spray-dried Lactobacillus plantarum TA and 7A after two years of storage by using real-time PCR. in CEFood 2012 - Proceedings of 6th Central European Congress on Food 6th Central European Congress on Food, CEFood 2012., 1082-1086. https://hdl.handle.net/21.15107/rcub_agrospace_2776
Mirković N, Radulović Z, Matijašić B, Petrušić MM, Petrović T, Djordjević V, Nedović V. Quantification of viable spray-dried Lactobacillus plantarum TA and 7A after two years of storage by using real-time PCR. in CEFood 2012 - Proceedings of 6th Central European Congress on Food. 2012;:1082-1086. https://hdl.handle.net/21.15107/rcub_agrospace_2776 .
Mirković, Nemanja, Radulović, Zorica, Matijašić, B.J.B., Petrušić, Milica M., Petrović, Tanja, Djordjević, Verica, Nedović, Viktor, "Quantification of viable spray-dried Lactobacillus plantarum TA and 7A after two years of storage by using real-time PCR" in CEFood 2012 - Proceedings of 6th Central European Congress on Food (2012):1082-1086, https://hdl.handle.net/21.15107/rcub_agrospace_2776 .