Cold-Pressed Pumpkin Seed Oil Antioxidant Activity as Determined by a DC Polarographic Assay Based on Hydrogen Peroxide Scavenge

Gorjanović, Stanislava; Rabrenović, Biljana; Novaković, Miroslav M.; Dimić, Etelka; Basić, Zorica N.; Suznjević, Desanka Z.

doi:10.1007/s11746-011-1863-3

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2011

Authors

Gorjanović, Stanislava
Rabrenović, Biljana

Novaković, Miroslav M.
Dimić, Etelka
Basić, Zorica N.
Suznjević, Desanka Z.

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Abstract

Antioxidant (AO) activity of cold pressed pumpkin (Cucurbita pepo L.) seed oil, produced from three naked and one hulled variety, was assessed using a DC polarographic assay based on a hydrogen peroxide scavenge (HPS). Results are expressed as the decrease of the anodic oxidation current of hydrogen peroxide obtained upon addition of methanolic extract of the investigated oils. Strict correlations of HPS and (1) radical scavenging capacity against the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) (0.99), (2) the induction period estimated by a Rancimat test (0.99) and (3) total phenolic content estimated by Folin-Ciocalteu (FC) assay (0.99) were obtained. In addition, a significant correlation of HPS and the content of delta-tocopherol (0.87), squalene (0.67) and color CIE a* (-0.89) was found. Based on the results reported, the polarographic assay was found to be suitable for determination of AO activity as an indicator of the quality and oxidative stability of oil.

Keywords:

Antioxidant / Hydrogen peroxide / DC polarography / Oxidative stability / Phenolics / Pumpkin oil

Source:
Journal of the American Oil Chemists Society, 2011, 88, 12, 1875-1882

Publisher:

Wiley, Hoboken

Funding / projects:

DOI: 10.1007/s11746-011-1863-3

ISSN: 0003-021X

WoS: 000297128200004

Scopus: 2-s2.0-84855649683

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	18
	13

TY  - JOUR
AU  - Gorjanović, Stanislava
AU  - Rabrenović, Biljana
AU  - Novaković, Miroslav M.
AU  - Dimić, Etelka
AU  - Basić, Zorica N.
AU  - Suznjević, Desanka Z.
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2713
AB  - Antioxidant (AO) activity of cold pressed pumpkin (Cucurbita pepo L.) seed oil, produced from three naked and one hulled variety, was assessed using a DC polarographic assay based on a hydrogen peroxide scavenge (HPS). Results are expressed as the decrease of the anodic oxidation current of hydrogen peroxide obtained upon addition of methanolic extract of the investigated oils. Strict correlations of HPS and (1) radical scavenging capacity against the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) (0.99), (2) the induction period estimated by a Rancimat test (0.99) and (3) total phenolic content estimated by Folin-Ciocalteu (FC) assay (0.99) were obtained. In addition, a significant correlation of HPS and the content of delta-tocopherol (0.87), squalene (0.67) and color CIE a* (-0.89) was found. Based on the results reported, the polarographic assay was found to be suitable for determination of AO activity as an indicator of the quality and oxidative stability of oil.
PB  - Wiley, Hoboken
T2  - Journal of the American Oil Chemists Society
T1  - Cold-Pressed Pumpkin Seed Oil Antioxidant Activity as Determined by a DC Polarographic Assay Based on Hydrogen Peroxide Scavenge
EP  - 1882
IS  - 12
SP  - 1875
VL  - 88
DO  - 10.1007/s11746-011-1863-3
ER  -

@article{
author = "Gorjanović, Stanislava and Rabrenović, Biljana and Novaković, Miroslav M. and Dimić, Etelka and Basić, Zorica N. and Suznjević, Desanka Z.",
year = "2011",
abstract = "Antioxidant (AO) activity of cold pressed pumpkin (Cucurbita pepo L.) seed oil, produced from three naked and one hulled variety, was assessed using a DC polarographic assay based on a hydrogen peroxide scavenge (HPS). Results are expressed as the decrease of the anodic oxidation current of hydrogen peroxide obtained upon addition of methanolic extract of the investigated oils. Strict correlations of HPS and (1) radical scavenging capacity against the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) (0.99), (2) the induction period estimated by a Rancimat test (0.99) and (3) total phenolic content estimated by Folin-Ciocalteu (FC) assay (0.99) were obtained. In addition, a significant correlation of HPS and the content of delta-tocopherol (0.87), squalene (0.67) and color CIE a* (-0.89) was found. Based on the results reported, the polarographic assay was found to be suitable for determination of AO activity as an indicator of the quality and oxidative stability of oil.",
publisher = "Wiley, Hoboken",
journal = "Journal of the American Oil Chemists Society",
title = "Cold-Pressed Pumpkin Seed Oil Antioxidant Activity as Determined by a DC Polarographic Assay Based on Hydrogen Peroxide Scavenge",
pages = "1882-1875",
number = "12",
volume = "88",
doi = "10.1007/s11746-011-1863-3"
}

Gorjanović, S., Rabrenović, B., Novaković, M. M., Dimić, E., Basić, Z. N.,& Suznjević, D. Z.. (2011). Cold-Pressed Pumpkin Seed Oil Antioxidant Activity as Determined by a DC Polarographic Assay Based on Hydrogen Peroxide Scavenge. in Journal of the American Oil Chemists Society
Wiley, Hoboken., 88(12), 1875-1882.
https://doi.org/10.1007/s11746-011-1863-3

Gorjanović S, Rabrenović B, Novaković MM, Dimić E, Basić ZN, Suznjević DZ. Cold-Pressed Pumpkin Seed Oil Antioxidant Activity as Determined by a DC Polarographic Assay Based on Hydrogen Peroxide Scavenge. in Journal of the American Oil Chemists Society. 2011;88(12):1875-1882.
doi:10.1007/s11746-011-1863-3 .

Gorjanović, Stanislava, Rabrenović, Biljana, Novaković, Miroslav M., Dimić, Etelka, Basić, Zorica N., Suznjević, Desanka Z., "Cold-Pressed Pumpkin Seed Oil Antioxidant Activity as Determined by a DC Polarographic Assay Based on Hydrogen Peroxide Scavenge" in Journal of the American Oil Chemists Society, 88, no. 12 (2011):1875-1882,
https://doi.org/10.1007/s11746-011-1863-3 . .