Cold-Pressed Pumpkin Seed Oil Antioxidant Activity as Determined by a DC Polarographic Assay Based on Hydrogen Peroxide Scavenge
Authorized Users Only
2011
Authors
Gorjanović, StanislavaRabrenović, Biljana

Novaković, Miroslav M.
Dimić, Etelka
Basić, Zorica N.
Suznjević, Desanka Z.
Article (Published version)

Metadata
Show full item recordAbstract
Antioxidant (AO) activity of cold pressed pumpkin (Cucurbita pepo L.) seed oil, produced from three naked and one hulled variety, was assessed using a DC polarographic assay based on a hydrogen peroxide scavenge (HPS). Results are expressed as the decrease of the anodic oxidation current of hydrogen peroxide obtained upon addition of methanolic extract of the investigated oils. Strict correlations of HPS and (1) radical scavenging capacity against the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) (0.99), (2) the induction period estimated by a Rancimat test (0.99) and (3) total phenolic content estimated by Folin-Ciocalteu (FC) assay (0.99) were obtained. In addition, a significant correlation of HPS and the content of delta-tocopherol (0.87), squalene (0.67) and color CIE a* (-0.89) was found. Based on the results reported, the polarographic assay was found to be suitable for determination of AO activity as an indicator of the quality and oxidative stability of oil.
Keywords:
Antioxidant / Hydrogen peroxide / DC polarography / Oxidative stability / Phenolics / Pumpkin oilSource:
Journal of the American Oil Chemists Society, 2011, 88, 12, 1875-1882Publisher:
- Wiley, Hoboken
Funding / projects:
- Content of bioactive components in small and stone fruits as affected by cultivar specificities and growing conditions, and obtaining biologically valuable products by improved and newly developed technologies (RS-31093)
- Modulation of antioxidative metabolism in plants for improvement of plant abiotic stress tolerance and identification of new biomarkers for application in remediation and monitoring of degraded biotopes (RS-43010)
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-172053)
DOI: 10.1007/s11746-011-1863-3
ISSN: 0003-021X
WoS: 000297128200004
Scopus: 2-s2.0-84855649683
Collections
Institution/Community
Poljoprivredni fakultetTY - JOUR AU - Gorjanović, Stanislava AU - Rabrenović, Biljana AU - Novaković, Miroslav M. AU - Dimić, Etelka AU - Basić, Zorica N. AU - Suznjević, Desanka Z. PY - 2011 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/2713 AB - Antioxidant (AO) activity of cold pressed pumpkin (Cucurbita pepo L.) seed oil, produced from three naked and one hulled variety, was assessed using a DC polarographic assay based on a hydrogen peroxide scavenge (HPS). Results are expressed as the decrease of the anodic oxidation current of hydrogen peroxide obtained upon addition of methanolic extract of the investigated oils. Strict correlations of HPS and (1) radical scavenging capacity against the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) (0.99), (2) the induction period estimated by a Rancimat test (0.99) and (3) total phenolic content estimated by Folin-Ciocalteu (FC) assay (0.99) were obtained. In addition, a significant correlation of HPS and the content of delta-tocopherol (0.87), squalene (0.67) and color CIE a* (-0.89) was found. Based on the results reported, the polarographic assay was found to be suitable for determination of AO activity as an indicator of the quality and oxidative stability of oil. PB - Wiley, Hoboken T2 - Journal of the American Oil Chemists Society T1 - Cold-Pressed Pumpkin Seed Oil Antioxidant Activity as Determined by a DC Polarographic Assay Based on Hydrogen Peroxide Scavenge EP - 1882 IS - 12 SP - 1875 VL - 88 DO - 10.1007/s11746-011-1863-3 ER -
@article{ author = "Gorjanović, Stanislava and Rabrenović, Biljana and Novaković, Miroslav M. and Dimić, Etelka and Basić, Zorica N. and Suznjević, Desanka Z.", year = "2011", abstract = "Antioxidant (AO) activity of cold pressed pumpkin (Cucurbita pepo L.) seed oil, produced from three naked and one hulled variety, was assessed using a DC polarographic assay based on a hydrogen peroxide scavenge (HPS). Results are expressed as the decrease of the anodic oxidation current of hydrogen peroxide obtained upon addition of methanolic extract of the investigated oils. Strict correlations of HPS and (1) radical scavenging capacity against the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) (0.99), (2) the induction period estimated by a Rancimat test (0.99) and (3) total phenolic content estimated by Folin-Ciocalteu (FC) assay (0.99) were obtained. In addition, a significant correlation of HPS and the content of delta-tocopherol (0.87), squalene (0.67) and color CIE a* (-0.89) was found. Based on the results reported, the polarographic assay was found to be suitable for determination of AO activity as an indicator of the quality and oxidative stability of oil.", publisher = "Wiley, Hoboken", journal = "Journal of the American Oil Chemists Society", title = "Cold-Pressed Pumpkin Seed Oil Antioxidant Activity as Determined by a DC Polarographic Assay Based on Hydrogen Peroxide Scavenge", pages = "1882-1875", number = "12", volume = "88", doi = "10.1007/s11746-011-1863-3" }
Gorjanović, S., Rabrenović, B., Novaković, M. M., Dimić, E., Basić, Z. N.,& Suznjević, D. Z.. (2011). Cold-Pressed Pumpkin Seed Oil Antioxidant Activity as Determined by a DC Polarographic Assay Based on Hydrogen Peroxide Scavenge. in Journal of the American Oil Chemists Society Wiley, Hoboken., 88(12), 1875-1882. https://doi.org/10.1007/s11746-011-1863-3
Gorjanović S, Rabrenović B, Novaković MM, Dimić E, Basić ZN, Suznjević DZ. Cold-Pressed Pumpkin Seed Oil Antioxidant Activity as Determined by a DC Polarographic Assay Based on Hydrogen Peroxide Scavenge. in Journal of the American Oil Chemists Society. 2011;88(12):1875-1882. doi:10.1007/s11746-011-1863-3 .
Gorjanović, Stanislava, Rabrenović, Biljana, Novaković, Miroslav M., Dimić, Etelka, Basić, Zorica N., Suznjević, Desanka Z., "Cold-Pressed Pumpkin Seed Oil Antioxidant Activity as Determined by a DC Polarographic Assay Based on Hydrogen Peroxide Scavenge" in Journal of the American Oil Chemists Society, 88, no. 12 (2011):1875-1882, https://doi.org/10.1007/s11746-011-1863-3 . .