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dc.creatorMilošević, Snežana
dc.creatorSubotić, Angelina
dc.creatorBulajić, Aleksandra
dc.creatorDjekić, Ivana
dc.creatorJevremović, Sladjana
dc.creatorVučurović, Ana
dc.creatorKrstić, Branka
dc.date.accessioned2020-12-17T20:12:08Z
dc.date.available2020-12-17T20:12:08Z
dc.date.issued2011
dc.identifier.issn0717-3458
dc.identifier.urihttp://aspace.agrif.bg.ac.rs/handle/123456789/2667
dc.description.abstractThe possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 mu M), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant pigments content in I. hawkerii shoots increased significantly in the presence of cytokinins. Rooting of shoots was spontaneous on the same media. Rooted plantlets were transferred to soil where 97% successfully acclimatized. By DAS-ELISA and RT-PCR, 80% of the in vitro plantlets were shown to be a virus-free. Considering these, the present protocol seems to be an efficient method for in vitro generation of virus-free I. hawkerii plantlets by meristem tip cultures.en
dc.publisherUniv Catolica De Valparaiso, Valparaiso
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31019/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceElectronic Journal of Biotechnology
dc.subjectmeristem cultureen
dc.subjectNew Guinea Impatiensen
dc.subjectTomato spotted wilt virusen
dc.subjectvirus-free plantsen
dc.titleElimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free planten
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.issue1
dc.citation.other14(1): -
dc.citation.rankM23
dc.citation.volume14
dc.identifier.doi10.2225/vol14-issue1-fulltext-5
dc.identifier.fulltexthttp://aspace.agrif.bg.ac.rs/bitstream/id/1419/2664.pdf
dc.identifier.scopus2-s2.0-79955491211
dc.identifier.wos000293630100003
dc.type.versionpublishedVersion


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