Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant

2011
Autori
Milošević, Snežana
Subotić, Angelina

Bulajić, Aleksandra

Djekić, Ivana

Jevremović, Sladjana

Vučurović, Ana

Krstić, Branka
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 mu M), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant pigments content in I. hawkerii shoots increased significantly in the presence of cytokinins. Rooting of shoots was spontaneous on the same media. Rooted plantlets were transferred to soil where 97% successfully acclimatized. By DAS-EL...ISA and RT-PCR, 80% of the in vitro plantlets were shown to be a virus-free. Considering these, the present protocol seems to be an efficient method for in vitro generation of virus-free I. hawkerii plantlets by meristem tip cultures.
Ključne reči:
meristem culture / New Guinea Impatiens / Tomato spotted wilt virus / virus-free plantsIzvor:
Electronic Journal of Biotechnology, 2011, 14, 1Izdavač:
- Univ Catolica De Valparaiso, Valparaiso
Finansiranje / projekti:
- Razvoj i primena biotehnoloških postupaka u dobijanju zdravog sadnog materijala ukrasnih biljaka (RS-31019)
DOI: 10.2225/vol14-issue1-fulltext-5
ISSN: 0717-3458
WoS: 000293630100003
Scopus: 2-s2.0-79955491211
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Milošević, Snežana AU - Subotić, Angelina AU - Bulajić, Aleksandra AU - Djekić, Ivana AU - Jevremović, Sladjana AU - Vučurović, Ana AU - Krstić, Branka PY - 2011 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/2667 AB - The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 mu M), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant pigments content in I. hawkerii shoots increased significantly in the presence of cytokinins. Rooting of shoots was spontaneous on the same media. Rooted plantlets were transferred to soil where 97% successfully acclimatized. By DAS-ELISA and RT-PCR, 80% of the in vitro plantlets were shown to be a virus-free. Considering these, the present protocol seems to be an efficient method for in vitro generation of virus-free I. hawkerii plantlets by meristem tip cultures. PB - Univ Catolica De Valparaiso, Valparaiso T2 - Electronic Journal of Biotechnology T1 - Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant IS - 1 VL - 14 DO - 10.2225/vol14-issue1-fulltext-5 ER -
@article{ author = "Milošević, Snežana and Subotić, Angelina and Bulajić, Aleksandra and Djekić, Ivana and Jevremović, Sladjana and Vučurović, Ana and Krstić, Branka", year = "2011", abstract = "The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 mu M), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant pigments content in I. hawkerii shoots increased significantly in the presence of cytokinins. Rooting of shoots was spontaneous on the same media. Rooted plantlets were transferred to soil where 97% successfully acclimatized. By DAS-ELISA and RT-PCR, 80% of the in vitro plantlets were shown to be a virus-free. Considering these, the present protocol seems to be an efficient method for in vitro generation of virus-free I. hawkerii plantlets by meristem tip cultures.", publisher = "Univ Catolica De Valparaiso, Valparaiso", journal = "Electronic Journal of Biotechnology", title = "Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant", number = "1", volume = "14", doi = "10.2225/vol14-issue1-fulltext-5" }
Milošević, S., Subotić, A., Bulajić, A., Djekić, I., Jevremović, S., Vučurović, A.,& Krstić, B.. (2011). Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant. in Electronic Journal of Biotechnology Univ Catolica De Valparaiso, Valparaiso., 14(1). https://doi.org/10.2225/vol14-issue1-fulltext-5
Milošević S, Subotić A, Bulajić A, Djekić I, Jevremović S, Vučurović A, Krstić B. Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant. in Electronic Journal of Biotechnology. 2011;14(1). doi:10.2225/vol14-issue1-fulltext-5 .
Milošević, Snežana, Subotić, Angelina, Bulajić, Aleksandra, Djekić, Ivana, Jevremović, Sladjana, Vučurović, Ana, Krstić, Branka, "Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant" in Electronic Journal of Biotechnology, 14, no. 1 (2011), https://doi.org/10.2225/vol14-issue1-fulltext-5 . .