The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 mu M), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant pigments content in I. hawkerii shoots increased significantly in the presence of cytokinins. Rooting of shoots was spontaneous on the same media. Rooted plantlets were transferred to soil where 97% successfully acclimatized. By DAS-EL...ISA and RT-PCR, 80% of the in vitro plantlets were shown to be a virus-free. Considering these, the present protocol seems to be an efficient method for in vitro generation of virus-free I. hawkerii plantlets by meristem tip cultures.
TY - JOUR
AU - Milošević, Snežana
AU - Subotić, Angelina
AU - Bulajić, Aleksandra
AU - Djekić, Ivana
AU - Jevremović, Sladjana
AU - Vučurović, Ana
AU - Krstić, Branka
PY - 2011
UR - http://aspace.agrif.bg.ac.rs/handle/123456789/2667
AB - The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 mu M), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant pigments content in I. hawkerii shoots increased significantly in the presence of cytokinins. Rooting of shoots was spontaneous on the same media. Rooted plantlets were transferred to soil where 97% successfully acclimatized. By DAS-ELISA and RT-PCR, 80% of the in vitro plantlets were shown to be a virus-free. Considering these, the present protocol seems to be an efficient method for in vitro generation of virus-free I. hawkerii plantlets by meristem tip cultures.
PB - Univ Catolica De Valparaiso, Valparaiso
T2 - Electronic Journal of Biotechnology
T1 - Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant
IS - 1
VL - 14
DO - 10.2225/vol14-issue1-fulltext-5
ER -
@article{
author = "Milošević, Snežana and Subotić, Angelina and Bulajić, Aleksandra and Djekić, Ivana and Jevremović, Sladjana and Vučurović, Ana and Krstić, Branka",
year = "2011",
abstract = "The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 mu M), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant pigments content in I. hawkerii shoots increased significantly in the presence of cytokinins. Rooting of shoots was spontaneous on the same media. Rooted plantlets were transferred to soil where 97% successfully acclimatized. By DAS-ELISA and RT-PCR, 80% of the in vitro plantlets were shown to be a virus-free. Considering these, the present protocol seems to be an efficient method for in vitro generation of virus-free I. hawkerii plantlets by meristem tip cultures.",
publisher = "Univ Catolica De Valparaiso, Valparaiso",
journal = "Electronic Journal of Biotechnology",
title = "Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant",
number = "1",
volume = "14",
doi = "10.2225/vol14-issue1-fulltext-5"
}
Milošević, S., Subotić, A., Bulajić, A., Djekić, I., Jevremović, S., Vučurović, A.,& Krstić, B.. (2011). Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant. in Electronic Journal of Biotechnology
Univ Catolica De Valparaiso, Valparaiso., 14(1).
https://doi.org/10.2225/vol14-issue1-fulltext-5
Milošević S, Subotić A, Bulajić A, Djekić I, Jevremović S, Vučurović A, Krstić B. Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant. in Electronic Journal of Biotechnology. 2011;14(1).
doi:10.2225/vol14-issue1-fulltext-5 .
Milošević, Snežana, Subotić, Angelina, Bulajić, Aleksandra, Djekić, Ivana, Jevremović, Sladjana, Vučurović, Ana, Krstić, Branka, "Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant" in Electronic Journal of Biotechnology, 14, no. 1 (2011),
https://doi.org/10.2225/vol14-issue1-fulltext-5 . .
