Improved Solvent Extraction Procedure and High-Performance Liquid Chromatography-Evaporative Light-Scattering Detector Method for Analysis of Polar Lipids from Dairy Materials
Само за регистроване кориснике
2011
Аутори
Thien Trung LeMiočinović, Jelena
Tuyet Mai Nguyen
Rombaut, Roeland
van Camp, John
Dewettinck, Koen
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A normal-phase high-performance liquid chromatography-evaporative light-scattering detector method employing dichloromethane, methanol, and acetic acid/triethylamine buffer as the mobile phase was developed for analysis of polar lipids (PLs). This method was applicable for analysis of PLs from both dairy materials and soy lecithin. All of the PLs of interest such as glycolipids, phospholipids, and sphingomyelin were well separated with a total run time of 22.5 min and without necessitating the removal of neutral lipids beforehand. Peak retention times were stable, and the method was reproducible. In this study, a modified method of using solvents for extraction of PLs from dairy matrices was also investigated. The modified method offered higher extraction efficiency, consumed less time, and in some cases saved solvent use.
Кључне речи:
Polar lipids / phospholipids / HPLC / ELSD / extraction / MFGMИзвор:
Journal of Agricultural and Food Chemistry, 2011, 59, 19, 10407-10413Издавач:
- Amer Chemical Soc, Washington
Финансирање / пројекти:
- BOF of Ghent University, BelgiumGhent University [BOF07/DOS/047]
- FIN (Fund for Scientific Research), Flanders [G.0490.08]
DOI: 10.1021/jf200202d
ISSN: 0021-8561
PubMed: 21877694
WoS: 000295488000002
Scopus: 2-s2.0-80053543112
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Thien Trung Le AU - Miočinović, Jelena AU - Tuyet Mai Nguyen AU - Rombaut, Roeland AU - van Camp, John AU - Dewettinck, Koen PY - 2011 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/2648 AB - A normal-phase high-performance liquid chromatography-evaporative light-scattering detector method employing dichloromethane, methanol, and acetic acid/triethylamine buffer as the mobile phase was developed for analysis of polar lipids (PLs). This method was applicable for analysis of PLs from both dairy materials and soy lecithin. All of the PLs of interest such as glycolipids, phospholipids, and sphingomyelin were well separated with a total run time of 22.5 min and without necessitating the removal of neutral lipids beforehand. Peak retention times were stable, and the method was reproducible. In this study, a modified method of using solvents for extraction of PLs from dairy matrices was also investigated. The modified method offered higher extraction efficiency, consumed less time, and in some cases saved solvent use. PB - Amer Chemical Soc, Washington T2 - Journal of Agricultural and Food Chemistry T1 - Improved Solvent Extraction Procedure and High-Performance Liquid Chromatography-Evaporative Light-Scattering Detector Method for Analysis of Polar Lipids from Dairy Materials EP - 10413 IS - 19 SP - 10407 VL - 59 DO - 10.1021/jf200202d ER -
@article{ author = "Thien Trung Le and Miočinović, Jelena and Tuyet Mai Nguyen and Rombaut, Roeland and van Camp, John and Dewettinck, Koen", year = "2011", abstract = "A normal-phase high-performance liquid chromatography-evaporative light-scattering detector method employing dichloromethane, methanol, and acetic acid/triethylamine buffer as the mobile phase was developed for analysis of polar lipids (PLs). This method was applicable for analysis of PLs from both dairy materials and soy lecithin. All of the PLs of interest such as glycolipids, phospholipids, and sphingomyelin were well separated with a total run time of 22.5 min and without necessitating the removal of neutral lipids beforehand. Peak retention times were stable, and the method was reproducible. In this study, a modified method of using solvents for extraction of PLs from dairy matrices was also investigated. The modified method offered higher extraction efficiency, consumed less time, and in some cases saved solvent use.", publisher = "Amer Chemical Soc, Washington", journal = "Journal of Agricultural and Food Chemistry", title = "Improved Solvent Extraction Procedure and High-Performance Liquid Chromatography-Evaporative Light-Scattering Detector Method for Analysis of Polar Lipids from Dairy Materials", pages = "10413-10407", number = "19", volume = "59", doi = "10.1021/jf200202d" }
Thien Trung Le, Miočinović, J., Tuyet Mai Nguyen, Rombaut, R., van Camp, J.,& Dewettinck, K.. (2011). Improved Solvent Extraction Procedure and High-Performance Liquid Chromatography-Evaporative Light-Scattering Detector Method for Analysis of Polar Lipids from Dairy Materials. in Journal of Agricultural and Food Chemistry Amer Chemical Soc, Washington., 59(19), 10407-10413. https://doi.org/10.1021/jf200202d
Thien Trung Le, Miočinović J, Tuyet Mai Nguyen, Rombaut R, van Camp J, Dewettinck K. Improved Solvent Extraction Procedure and High-Performance Liquid Chromatography-Evaporative Light-Scattering Detector Method for Analysis of Polar Lipids from Dairy Materials. in Journal of Agricultural and Food Chemistry. 2011;59(19):10407-10413. doi:10.1021/jf200202d .
Thien Trung Le, Miočinović, Jelena, Tuyet Mai Nguyen, Rombaut, Roeland, van Camp, John, Dewettinck, Koen, "Improved Solvent Extraction Procedure and High-Performance Liquid Chromatography-Evaporative Light-Scattering Detector Method for Analysis of Polar Lipids from Dairy Materials" in Journal of Agricultural and Food Chemistry, 59, no. 19 (2011):10407-10413, https://doi.org/10.1021/jf200202d . .