Antioxidant properties of hot water extracts from carpophore and spores of mushroom Ganoderma lucidum

Abstract

Ganoderma lucidum (Leyss.:Fr.) Karst is one of the medicinal mushrooms, which possesses enviable antioxidant properties. Objective of this investigation was to evaluate antioxidant activity, reducing power, scavenging abilities on 1.1-diphenyl-2-picrylhydrazyl (DPPH) radicals and chelating effects on ferrous ions of hot water extracts obtained from carpophore and spores of this mushroom. Hot water extract from carpophore (Gl-I) showed high antioxidant activity of 85.7 ± 0.7%, at 10 mg/ml, while antioxidant activity of hot water extract from spores (Gl-Is) was 9.2 ± 0.3% at 10 mg/ml. Reducing power of Gl-I reached a plateau of 3.4 ± 0.1 at 20 mg/ml, and 0.3 ± 0.0 at 20 mg/ml for Gl-Is. At 10 mg/ml, scavenging ability on DPPH radicals of Gl-I increased to 96.8 ± 2.5%, whereas Gl-Is scavenged DPPH radicals by 69.6 ± 2.5% at 10 mg/ml. Gl-I chelated 81.6 ± 3.6 % of ferrous ions at 20 mg/ml, while the chelating effect of Gl-Is was 73.8 ± 1.7%. The antioxidative activities of hot water extracts from carpophore and spores of the mushroom G. lucidum were concentration dependent and increased with an increase in the concentration.

Ganoderma lucidum (Leyss.: Fr.) Karst je jedna od medicinski važnih gljiva, koja poseduje izuzetna antioksidativna svojstva. Cilj ovog istraživanja bio je potvrda antioksidativne aktivnosti, redukcione sposobnosti, mogućnosti hvatanja 1,1-difenil-2-pikrilhidrazil (DPPH) radikala i sposobnosti heliranja jona gvožđa vrelih vodenih ekstrakata dobijenih iz karpofora i spora ove gljive. Vreli vodeni ekstrakt iz karpofora (Gl-I) pokazao je visoku antioksidativnu aktivnost 85.7 ± 0.7%, pri 10 mg/ml, dok je antioksidativna aktivnost vrelog vodenog ekstrakta spora (Gl-Is) bila 9.2 ± 0.3% pri 10 mg/ml. Redukciona sposobnost ekstrakta Gl-I dostigla je nivo 3.4 ± 0.1 pri 20 mg/ml i 0.3 ± 0.0 pri 20 mg/ml za ekstrakt Gl-Is. Pri koncentraciji 10 mg/ml sposobnost hvatanja DPPH radikala ekstrakta Gl-I dostigla je 96.8 ± 2.5%, dok je ekstrakt Gl-Is vezao 69.6 ± 2.5% DPPH radikala pri 10 mg/ml. Ekstrakt Gl-I helirao je 81.6 ± 3.6 % fero jona pri koncentraciji 20 mg/ml, a helirajući efekat ekstrakta Gl-Is bio je 73.8 ± 1.7%. Antioksidativne aktivnosti vrelih vodenih ekstrakata iz karpofora i spora gljive G. lucidum zavisile su od koncentracije i povećavale su se sa povećanjem koncentracije.