Isoenzyme polymorphism of almond genotypes selected in the region of northern Serbia
Abstract
Isoenzyme polymorphism was studied in 20 almond (Prunus dulcis [Mill.] D. A. Webb) genotypes selected from seedling populations of unknown almond cultivars in the region of northern Serbia (Vojvodina). Fourteen enzyme systems were studied using the method of vertical polyacrylamide gel electrophoresis. Ten systems were polymorphic in twelve loci. This polymorphism allowed unique identification of all studied genotypes. The most useful enzyme for analysis of almond genetic variability was menadione reductase. Polymorphism identified for alkaline phosphatase, formate dehydrogenase, glutamate dehydrogenase, malic enzyme, and menadione reductase was reported for the first time in almond. Cluster analysis was used to construct a dendrogram on which five clusters with different number of genotypes could be identified.
Keywords:
Prunus dulcis / cluster analysis / electrophoresis / isoenzymesSource:
Horticultural Science, 2010, 37, 2, 56-61Publisher:
- Czech Academy Agricultural Sciences, Prague
Funding / projects:
- Nove sorte, selekcije i tehnologije gajenja kao faktori intenziviranja voćarske proizvodnje (RS-20103)
DOI: 10.17221/55/2009-HORTSCI
ISSN: 0862-867X
WoS: 000277823800003
Scopus: 2-s2.0-77952722056
Collections
Institution/Community
Poljoprivredni fakultetTY - JOUR AU - Čolić, Slavica AU - Milatović, Dragan AU - Nikolić, D. AU - Zec, Gordan PY - 2010 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/2390 AB - Isoenzyme polymorphism was studied in 20 almond (Prunus dulcis [Mill.] D. A. Webb) genotypes selected from seedling populations of unknown almond cultivars in the region of northern Serbia (Vojvodina). Fourteen enzyme systems were studied using the method of vertical polyacrylamide gel electrophoresis. Ten systems were polymorphic in twelve loci. This polymorphism allowed unique identification of all studied genotypes. The most useful enzyme for analysis of almond genetic variability was menadione reductase. Polymorphism identified for alkaline phosphatase, formate dehydrogenase, glutamate dehydrogenase, malic enzyme, and menadione reductase was reported for the first time in almond. Cluster analysis was used to construct a dendrogram on which five clusters with different number of genotypes could be identified. PB - Czech Academy Agricultural Sciences, Prague T2 - Horticultural Science T1 - Isoenzyme polymorphism of almond genotypes selected in the region of northern Serbia EP - 61 IS - 2 SP - 56 VL - 37 DO - 10.17221/55/2009-HORTSCI ER -
@article{ author = "Čolić, Slavica and Milatović, Dragan and Nikolić, D. and Zec, Gordan", year = "2010", abstract = "Isoenzyme polymorphism was studied in 20 almond (Prunus dulcis [Mill.] D. A. Webb) genotypes selected from seedling populations of unknown almond cultivars in the region of northern Serbia (Vojvodina). Fourteen enzyme systems were studied using the method of vertical polyacrylamide gel electrophoresis. Ten systems were polymorphic in twelve loci. This polymorphism allowed unique identification of all studied genotypes. The most useful enzyme for analysis of almond genetic variability was menadione reductase. Polymorphism identified for alkaline phosphatase, formate dehydrogenase, glutamate dehydrogenase, malic enzyme, and menadione reductase was reported for the first time in almond. Cluster analysis was used to construct a dendrogram on which five clusters with different number of genotypes could be identified.", publisher = "Czech Academy Agricultural Sciences, Prague", journal = "Horticultural Science", title = "Isoenzyme polymorphism of almond genotypes selected in the region of northern Serbia", pages = "61-56", number = "2", volume = "37", doi = "10.17221/55/2009-HORTSCI" }
Čolić, S., Milatović, D., Nikolić, D.,& Zec, G.. (2010). Isoenzyme polymorphism of almond genotypes selected in the region of northern Serbia. in Horticultural Science Czech Academy Agricultural Sciences, Prague., 37(2), 56-61. https://doi.org/10.17221/55/2009-HORTSCI
Čolić S, Milatović D, Nikolić D, Zec G. Isoenzyme polymorphism of almond genotypes selected in the region of northern Serbia. in Horticultural Science. 2010;37(2):56-61. doi:10.17221/55/2009-HORTSCI .
Čolić, Slavica, Milatović, Dragan, Nikolić, D., Zec, Gordan, "Isoenzyme polymorphism of almond genotypes selected in the region of northern Serbia" in Horticultural Science, 37, no. 2 (2010):56-61, https://doi.org/10.17221/55/2009-HORTSCI . .