Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory
Апстракт
The mechanism of alginate droplet formation as well as experimental parameters for producing very small polymer microbeads (i,e, less then 100 microns diameter) using an electrostatic droplet generator were investigated, It was found that microbead size was a function of needle diameter, charge arrangement (i,e, electrode geometry and spacing) and strength of electric field. The process of alginate droplet formation under the influence of electrostatic forces was assessed with an image analysis/video system and revealed distinct stages; After a voltage was applied the liquid meniscus at the needle tip was distorted from a spherical shape into an inverted cone-like shape. Alginate solution flowed into this cone at an increasing rate causing formation of a neck-like filament, When this filament broke away, producing small droplets, the meniscus relaxed back to a spherical shape until now of the polymer caused the process to start again, A mathematical model of droplet formation at the el...ectrified needle was developed from an analysis of the forces acting on a charged droplet, and agreed well with experimental results. Finally, to assess the effect of an electric field on animal cell viability, an insect cell suspension was subjected to a high voltage, There was no detectable loss in cell viability after the voltage was applied.
Кључне речи:
electrostatics / droplet generation / microbead / scale-up / modeling / animal cellsИзвор:
Minerva Biotecnologica, 2000, 12, 4, 241-248Издавач:
- Edizioni Minerva Medica, Turin
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Pjanović, Rada AU - Goosen, MFA AU - Nedović, Viktor AU - Bugarski, Branko PY - 2000 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/234 AB - The mechanism of alginate droplet formation as well as experimental parameters for producing very small polymer microbeads (i,e, less then 100 microns diameter) using an electrostatic droplet generator were investigated, It was found that microbead size was a function of needle diameter, charge arrangement (i,e, electrode geometry and spacing) and strength of electric field. The process of alginate droplet formation under the influence of electrostatic forces was assessed with an image analysis/video system and revealed distinct stages; After a voltage was applied the liquid meniscus at the needle tip was distorted from a spherical shape into an inverted cone-like shape. Alginate solution flowed into this cone at an increasing rate causing formation of a neck-like filament, When this filament broke away, producing small droplets, the meniscus relaxed back to a spherical shape until now of the polymer caused the process to start again, A mathematical model of droplet formation at the electrified needle was developed from an analysis of the forces acting on a charged droplet, and agreed well with experimental results. Finally, to assess the effect of an electric field on animal cell viability, an insect cell suspension was subjected to a high voltage, There was no detectable loss in cell viability after the voltage was applied. PB - Edizioni Minerva Medica, Turin T2 - Minerva Biotecnologica T1 - Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory EP - 248 IS - 4 SP - 241 VL - 12 UR - https://hdl.handle.net/21.15107/rcub_agrospace_234 ER -
@article{ author = "Pjanović, Rada and Goosen, MFA and Nedović, Viktor and Bugarski, Branko", year = "2000", abstract = "The mechanism of alginate droplet formation as well as experimental parameters for producing very small polymer microbeads (i,e, less then 100 microns diameter) using an electrostatic droplet generator were investigated, It was found that microbead size was a function of needle diameter, charge arrangement (i,e, electrode geometry and spacing) and strength of electric field. The process of alginate droplet formation under the influence of electrostatic forces was assessed with an image analysis/video system and revealed distinct stages; After a voltage was applied the liquid meniscus at the needle tip was distorted from a spherical shape into an inverted cone-like shape. Alginate solution flowed into this cone at an increasing rate causing formation of a neck-like filament, When this filament broke away, producing small droplets, the meniscus relaxed back to a spherical shape until now of the polymer caused the process to start again, A mathematical model of droplet formation at the electrified needle was developed from an analysis of the forces acting on a charged droplet, and agreed well with experimental results. Finally, to assess the effect of an electric field on animal cell viability, an insect cell suspension was subjected to a high voltage, There was no detectable loss in cell viability after the voltage was applied.", publisher = "Edizioni Minerva Medica, Turin", journal = "Minerva Biotecnologica", title = "Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory", pages = "248-241", number = "4", volume = "12", url = "https://hdl.handle.net/21.15107/rcub_agrospace_234" }
Pjanović, R., Goosen, M., Nedović, V.,& Bugarski, B.. (2000). Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory. in Minerva Biotecnologica Edizioni Minerva Medica, Turin., 12(4), 241-248. https://hdl.handle.net/21.15107/rcub_agrospace_234
Pjanović R, Goosen M, Nedović V, Bugarski B. Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory. in Minerva Biotecnologica. 2000;12(4):241-248. https://hdl.handle.net/21.15107/rcub_agrospace_234 .
Pjanović, Rada, Goosen, MFA, Nedović, Viktor, Bugarski, Branko, "Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory" in Minerva Biotecnologica, 12, no. 4 (2000):241-248, https://hdl.handle.net/21.15107/rcub_agrospace_234 .