Isolation of chromium resistant bacteria from a former bauxite mine area and their capacity for Cr (VI) reduction
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Raičević, Vera
Golić, Zorica
Lalević, Blažo

Jovanović, Ljubinko
Kiković, Dragan
Antić-Mladenović, Svetlana

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The Cr (VI) reducing capacity of bacteria has been investigated in many different soils and waters but little or no information is available from soils originating from bauxite mine areas. From soil, mud and rhizospheres of the floating aquatic plant Potamogeton natans L. and the terrestrial plant Carduus acanthoides L., the Cr content was determined and the microbial populations were sampled. The highest total chromium concentration (204.6 mgkg(-1)) was found in the rhizosphere of C. acanthoides. To determine the numbers and percentages of chromate-resistant bacteria, the autochthonous microbial populations were subjected to different Cr (VI) concentrations (40, 100, 300 and 1000 mM as K2Cr2O7). At 1000 mM Cr (VI) in the medium, about 25% of bacteria from soil and 45% of bacteria from the rhizospheres were resistant. Of 34 bacterial isolates, within 24 h, only Bacillus stearothermophilus 12 ms, Pseudomonas sp. 12 bk3 and Serratia fonticola 7 be were able to reduce 50 mu M Cr (VI). Usi...ng prolonged 72 h incubation, they were able to reduce 500 mu M Cr (VI) concentrations added to the medium. These chromate-resistant bacteria isolated from rhizosphere of plants growing in bauxite mine soil have great potential for bioremediation of Cr (VI)-polluted wastes.
Keywords:
Chromate-resistant bacteria / chromate reduction / bauxite mine / rhizosphere / plantsSource:
African Journal of Biotechnology, 2010, 9, 40, 6727-6732Collections
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Poljoprivredni fakultetTY - JOUR AU - Raičević, Vera AU - Golić, Zorica AU - Lalević, Blažo AU - Jovanović, Ljubinko AU - Kiković, Dragan AU - Antić-Mladenović, Svetlana PY - 2010 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/2141 AB - The Cr (VI) reducing capacity of bacteria has been investigated in many different soils and waters but little or no information is available from soils originating from bauxite mine areas. From soil, mud and rhizospheres of the floating aquatic plant Potamogeton natans L. and the terrestrial plant Carduus acanthoides L., the Cr content was determined and the microbial populations were sampled. The highest total chromium concentration (204.6 mgkg(-1)) was found in the rhizosphere of C. acanthoides. To determine the numbers and percentages of chromate-resistant bacteria, the autochthonous microbial populations were subjected to different Cr (VI) concentrations (40, 100, 300 and 1000 mM as K2Cr2O7). At 1000 mM Cr (VI) in the medium, about 25% of bacteria from soil and 45% of bacteria from the rhizospheres were resistant. Of 34 bacterial isolates, within 24 h, only Bacillus stearothermophilus 12 ms, Pseudomonas sp. 12 bk3 and Serratia fonticola 7 be were able to reduce 50 mu M Cr (VI). Using prolonged 72 h incubation, they were able to reduce 500 mu M Cr (VI) concentrations added to the medium. These chromate-resistant bacteria isolated from rhizosphere of plants growing in bauxite mine soil have great potential for bioremediation of Cr (VI)-polluted wastes. T2 - African Journal of Biotechnology T1 - Isolation of chromium resistant bacteria from a former bauxite mine area and their capacity for Cr (VI) reduction EP - 6732 IS - 40 SP - 6727 VL - 9 UR - https://hdl.handle.net/21.15107/rcub_agrospace_2141 ER -
@article{ author = "Raičević, Vera and Golić, Zorica and Lalević, Blažo and Jovanović, Ljubinko and Kiković, Dragan and Antić-Mladenović, Svetlana", year = "2010", abstract = "The Cr (VI) reducing capacity of bacteria has been investigated in many different soils and waters but little or no information is available from soils originating from bauxite mine areas. From soil, mud and rhizospheres of the floating aquatic plant Potamogeton natans L. and the terrestrial plant Carduus acanthoides L., the Cr content was determined and the microbial populations were sampled. The highest total chromium concentration (204.6 mgkg(-1)) was found in the rhizosphere of C. acanthoides. To determine the numbers and percentages of chromate-resistant bacteria, the autochthonous microbial populations were subjected to different Cr (VI) concentrations (40, 100, 300 and 1000 mM as K2Cr2O7). At 1000 mM Cr (VI) in the medium, about 25% of bacteria from soil and 45% of bacteria from the rhizospheres were resistant. Of 34 bacterial isolates, within 24 h, only Bacillus stearothermophilus 12 ms, Pseudomonas sp. 12 bk3 and Serratia fonticola 7 be were able to reduce 50 mu M Cr (VI). Using prolonged 72 h incubation, they were able to reduce 500 mu M Cr (VI) concentrations added to the medium. These chromate-resistant bacteria isolated from rhizosphere of plants growing in bauxite mine soil have great potential for bioremediation of Cr (VI)-polluted wastes.", journal = "African Journal of Biotechnology", title = "Isolation of chromium resistant bacteria from a former bauxite mine area and their capacity for Cr (VI) reduction", pages = "6732-6727", number = "40", volume = "9", url = "https://hdl.handle.net/21.15107/rcub_agrospace_2141" }
Raičević, V., Golić, Z., Lalević, B., Jovanović, L., Kiković, D.,& Antić-Mladenović, S.. (2010). Isolation of chromium resistant bacteria from a former bauxite mine area and their capacity for Cr (VI) reduction. in African Journal of Biotechnology, 9(40), 6727-6732. https://hdl.handle.net/21.15107/rcub_agrospace_2141
Raičević V, Golić Z, Lalević B, Jovanović L, Kiković D, Antić-Mladenović S. Isolation of chromium resistant bacteria from a former bauxite mine area and their capacity for Cr (VI) reduction. in African Journal of Biotechnology. 2010;9(40):6727-6732. https://hdl.handle.net/21.15107/rcub_agrospace_2141 .
Raičević, Vera, Golić, Zorica, Lalević, Blažo, Jovanović, Ljubinko, Kiković, Dragan, Antić-Mladenović, Svetlana, "Isolation of chromium resistant bacteria from a former bauxite mine area and their capacity for Cr (VI) reduction" in African Journal of Biotechnology, 9, no. 40 (2010):6727-6732, https://hdl.handle.net/21.15107/rcub_agrospace_2141 .