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dc.creatorŠmigić, Nada
dc.creatorRajković, Andreja
dc.creatorNielsen, Dennis Sandris
dc.creatorSiegumfeldt, Henrik
dc.creatorUyttendaele, Mieke
dc.creatorDevlieghere, Frank
dc.creatorArneborg, Nils
dc.date.accessioned2020-12-17T19:35:28Z
dc.date.available2020-12-17T19:35:28Z
dc.date.issued2009
dc.identifier.issn0168-1605
dc.identifier.urihttp://aspace.agrif.bg.ac.rs/handle/123456789/2071
dc.description.abstractThe aim of the study was to determine intracellular pH (pH(i)) as an indicator of the physiological state of two Campylobacter jejuni strains (603 and 608) at the single cell level after bactericidal treatment with lactic acid (3% v/v lactic acid, pH 4.0, 0.85% w/v NaCl) and during recovery and survival using Fluorescence Ratio Imaging Microscopy (FRIM). After exposure to lactic acid solution a decline in pHi to 5.5 (FRIM detection limit) was observed in the majority of cells (75-100%) within 2 min. The enumeration data revealed that after 2 min of lactic acid exposure, approx. 90% of the initial population became unculturable. In the following 10 min of exposure, a further decrease in the cell count was observed resulting in 3.53 and 3.21 log CFU/ml reduction of culturable cells at the end of the treatment. On the contrary, the FRIM results revealed that the subpopulations with pH(i >)5.5 increased between 2 and 12 min of exposure to lactic acid. Removing the acid stress and incubating the cells suspension under the more favourable conditions resulted in an immediate increase in cell population with pH(i)> pH(ex) for both C. jejuni strains. Further 24 h incubation at 37 degrees C resulted in increased pHi and colony count (recovery study). On the contrary, 24 h incubation at suboptimal temperature of 4 degrees C, showed pHi decrease to pH(ex)=6.0 (no pH gradient) in the whole population of C. jejuni cells. Rather than dying, cells exposed for longer time (72 and 120 h) to 4 degrees C increased the subpopulation of the cells with positive pH gradient, mostly comprised of the cells with Delta pH > 0.5, indicating the ability of C. jejuni cells to regulate their metabolic activity under suboptimal conditions.en
dc.publisherElsevier, Amsterdam
dc.relationEuropean CommissionEuropean CommissionEuropean Commission Joint Research Centre [FOOD-CT-2005-007081]
dc.rightsrestrictedAccess
dc.sourceInternational Journal of Food Microbiology
dc.subjectCampylobacter jejunien
dc.subjectIntracellular pHen
dc.subjectLactic acid decontaminationen
dc.subjectRecoveryen
dc.subjectSurvivalen
dc.titleIntracellular pH as an indicator of viability and resuscitation of Campylobacter jejuni after decontamination with lactic aciden
dc.typearticle
dc.rights.licenseARR
dc.citation.epage143
dc.citation.issue2
dc.citation.other135(2): 136-143
dc.citation.rankaM21
dc.citation.spage136
dc.citation.volume135
dc.identifier.doi10.1016/j.ijfoodmicro.2009.07.023
dc.identifier.rcubconv_4512
dc.identifier.scopus2-s2.0-70349254402
dc.identifier.pmid19720418
dc.identifier.wos000271051600009
dc.type.versionpublishedVersion


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