Intracellular pH as an indicator of viability and resuscitation of Campylobacter jejuni after decontamination with lactic acid
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2009
Authors
Šmigić, Nada
Rajković, Andreja

Nielsen, Dennis Sandris

Siegumfeldt, Henrik

Uyttendaele, Mieke
Devlieghere, Frank
Arneborg, Nils

Article (Published version)

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The aim of the study was to determine intracellular pH (pH(i)) as an indicator of the physiological state of two Campylobacter jejuni strains (603 and 608) at the single cell level after bactericidal treatment with lactic acid (3% v/v lactic acid, pH 4.0, 0.85% w/v NaCl) and during recovery and survival using Fluorescence Ratio Imaging Microscopy (FRIM). After exposure to lactic acid solution a decline in pHi to 5.5 (FRIM detection limit) was observed in the majority of cells (75-100%) within 2 min. The enumeration data revealed that after 2 min of lactic acid exposure, approx. 90% of the initial population became unculturable. In the following 10 min of exposure, a further decrease in the cell count was observed resulting in 3.53 and 3.21 log CFU/ml reduction of culturable cells at the end of the treatment. On the contrary, the FRIM results revealed that the subpopulations with pH(i >)5.5 increased between 2 and 12 min of exposure to lactic acid. Removing the acid stress and incubatin...g the cells suspension under the more favourable conditions resulted in an immediate increase in cell population with pH(i)> pH(ex) for both C. jejuni strains. Further 24 h incubation at 37 degrees C resulted in increased pHi and colony count (recovery study). On the contrary, 24 h incubation at suboptimal temperature of 4 degrees C, showed pHi decrease to pH(ex)=6.0 (no pH gradient) in the whole population of C. jejuni cells. Rather than dying, cells exposed for longer time (72 and 120 h) to 4 degrees C increased the subpopulation of the cells with positive pH gradient, mostly comprised of the cells with Delta pH > 0.5, indicating the ability of C. jejuni cells to regulate their metabolic activity under suboptimal conditions.
Keywords:
Campylobacter jejuni / Intracellular pH / Lactic acid decontamination / Recovery / SurvivalSource:
International Journal of Food Microbiology, 2009, 135, 2, 136-143Publisher:
- Elsevier, Amsterdam
Funding / projects:
- European CommissionEuropean CommissionEuropean Commission Joint Research Centre [FOOD-CT-2005-007081]
DOI: 10.1016/j.ijfoodmicro.2009.07.023
ISSN: 0168-1605
PubMed: 19720418
WoS: 000271051600009
Scopus: 2-s2.0-70349254402
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Poljoprivredni fakultetTY - JOUR AU - Šmigić, Nada AU - Rajković, Andreja AU - Nielsen, Dennis Sandris AU - Siegumfeldt, Henrik AU - Uyttendaele, Mieke AU - Devlieghere, Frank AU - Arneborg, Nils PY - 2009 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/2071 AB - The aim of the study was to determine intracellular pH (pH(i)) as an indicator of the physiological state of two Campylobacter jejuni strains (603 and 608) at the single cell level after bactericidal treatment with lactic acid (3% v/v lactic acid, pH 4.0, 0.85% w/v NaCl) and during recovery and survival using Fluorescence Ratio Imaging Microscopy (FRIM). After exposure to lactic acid solution a decline in pHi to 5.5 (FRIM detection limit) was observed in the majority of cells (75-100%) within 2 min. The enumeration data revealed that after 2 min of lactic acid exposure, approx. 90% of the initial population became unculturable. In the following 10 min of exposure, a further decrease in the cell count was observed resulting in 3.53 and 3.21 log CFU/ml reduction of culturable cells at the end of the treatment. On the contrary, the FRIM results revealed that the subpopulations with pH(i >)5.5 increased between 2 and 12 min of exposure to lactic acid. Removing the acid stress and incubating the cells suspension under the more favourable conditions resulted in an immediate increase in cell population with pH(i)> pH(ex) for both C. jejuni strains. Further 24 h incubation at 37 degrees C resulted in increased pHi and colony count (recovery study). On the contrary, 24 h incubation at suboptimal temperature of 4 degrees C, showed pHi decrease to pH(ex)=6.0 (no pH gradient) in the whole population of C. jejuni cells. Rather than dying, cells exposed for longer time (72 and 120 h) to 4 degrees C increased the subpopulation of the cells with positive pH gradient, mostly comprised of the cells with Delta pH > 0.5, indicating the ability of C. jejuni cells to regulate their metabolic activity under suboptimal conditions. PB - Elsevier, Amsterdam T2 - International Journal of Food Microbiology T1 - Intracellular pH as an indicator of viability and resuscitation of Campylobacter jejuni after decontamination with lactic acid EP - 143 IS - 2 SP - 136 VL - 135 DO - 10.1016/j.ijfoodmicro.2009.07.023 ER -
@article{ author = "Šmigić, Nada and Rajković, Andreja and Nielsen, Dennis Sandris and Siegumfeldt, Henrik and Uyttendaele, Mieke and Devlieghere, Frank and Arneborg, Nils", year = "2009", abstract = "The aim of the study was to determine intracellular pH (pH(i)) as an indicator of the physiological state of two Campylobacter jejuni strains (603 and 608) at the single cell level after bactericidal treatment with lactic acid (3% v/v lactic acid, pH 4.0, 0.85% w/v NaCl) and during recovery and survival using Fluorescence Ratio Imaging Microscopy (FRIM). After exposure to lactic acid solution a decline in pHi to 5.5 (FRIM detection limit) was observed in the majority of cells (75-100%) within 2 min. The enumeration data revealed that after 2 min of lactic acid exposure, approx. 90% of the initial population became unculturable. In the following 10 min of exposure, a further decrease in the cell count was observed resulting in 3.53 and 3.21 log CFU/ml reduction of culturable cells at the end of the treatment. On the contrary, the FRIM results revealed that the subpopulations with pH(i >)5.5 increased between 2 and 12 min of exposure to lactic acid. Removing the acid stress and incubating the cells suspension under the more favourable conditions resulted in an immediate increase in cell population with pH(i)> pH(ex) for both C. jejuni strains. Further 24 h incubation at 37 degrees C resulted in increased pHi and colony count (recovery study). On the contrary, 24 h incubation at suboptimal temperature of 4 degrees C, showed pHi decrease to pH(ex)=6.0 (no pH gradient) in the whole population of C. jejuni cells. Rather than dying, cells exposed for longer time (72 and 120 h) to 4 degrees C increased the subpopulation of the cells with positive pH gradient, mostly comprised of the cells with Delta pH > 0.5, indicating the ability of C. jejuni cells to regulate their metabolic activity under suboptimal conditions.", publisher = "Elsevier, Amsterdam", journal = "International Journal of Food Microbiology", title = "Intracellular pH as an indicator of viability and resuscitation of Campylobacter jejuni after decontamination with lactic acid", pages = "143-136", number = "2", volume = "135", doi = "10.1016/j.ijfoodmicro.2009.07.023" }
Šmigić, N., Rajković, A., Nielsen, D. S., Siegumfeldt, H., Uyttendaele, M., Devlieghere, F.,& Arneborg, N.. (2009). Intracellular pH as an indicator of viability and resuscitation of Campylobacter jejuni after decontamination with lactic acid. in International Journal of Food Microbiology Elsevier, Amsterdam., 135(2), 136-143. https://doi.org/10.1016/j.ijfoodmicro.2009.07.023
Šmigić N, Rajković A, Nielsen DS, Siegumfeldt H, Uyttendaele M, Devlieghere F, Arneborg N. Intracellular pH as an indicator of viability and resuscitation of Campylobacter jejuni after decontamination with lactic acid. in International Journal of Food Microbiology. 2009;135(2):136-143. doi:10.1016/j.ijfoodmicro.2009.07.023 .
Šmigić, Nada, Rajković, Andreja, Nielsen, Dennis Sandris, Siegumfeldt, Henrik, Uyttendaele, Mieke, Devlieghere, Frank, Arneborg, Nils, "Intracellular pH as an indicator of viability and resuscitation of Campylobacter jejuni after decontamination with lactic acid" in International Journal of Food Microbiology, 135, no. 2 (2009):136-143, https://doi.org/10.1016/j.ijfoodmicro.2009.07.023 . .