Influence of bioactive compounds extracted from mushroom Ganoderma lucidum on B and T cells

Abstract

Ganoderma lucidum (Leyss.: Fr.) Karst is one of the most often used mushrooms in traditional medicine of Far Eastern people. Because of its bitter taste and wooden build it is not suitable for nutrition, but the bioactive substances extracted from this mushroom possess very important medicinal characteristics. The aim of this experiment was to investigate the effects of different concentrations of isolated Ganoderma lucidum GL-I extract on the growth of JY (B) and Jurkat (T) cells. Obtained extracts were added to the cells in concentrations 1 mg/ml, 100 ng/ml, 10 ng/ml, 1 ng/ml and 100 pg/ml. JY and Jurkat cells were exposed to the action of bioactive compounds, b-glucans, during the incubation period of 72 h, at 37°C, in the atmosphere with 5% CO2 and their number was counted. Among all tested concentrations of extract the most important influence showed concentration of 1 mg/ml, which reduced the number of B cells by 61.46%, while in the case of T cells their number was reduced by 57.14%.

Ganoderma lucidum (Leyss.: Fr.) Karst je jedna od najčešće korišćenih gljiva u tradicionalnoj medicini naroda Dalekog istoka. Gorkog je ukusa i drvenaste građe, pa nije pogodna za ishranu, ali bioaktivne komponente ekstrakovane iz ove gljive pokazuju vrlo važne medicinske karakteristike. Cilj ovog rada bio je ispitivanje dejstva različitih koncentracija izdvojenog ekstrakta gljive Ganoderma lucidum GL-I na rast JY (B) i Jurkat (T) ćelija. Izdvojeni ekstrakt je dodat ćelijama u koncentracijama 1 mg/ml, 100 ng/ml, 10 ng/ml, 1 ng/ml i 100 pg/ml. JY i Jurkat ćelije su inkubirane 72 h na 37°C u atmosferi sa 5% SO2 u prisustvu bioaktivnih komponenata, polisaharida, b-glukana, a zatim je utvrđen njihov broj. Od svih primenjenih koncentracija ekstrakta najveći uticaj je pokazala koncentracija 1 mg/ml, koja je u slučaju dejstva na B ćelije dovela do smanjenja njihovog broja za 61.46%, a u slučaju dejstva na T ćelije broj je smanjen za 57.14%.