Influence of bioactive compounds extracted from mushroom Ganoderma lucidum on B and T cells
Uticaj bioaktivnih komponenata ekstrakovanih iz gljive Ganoderma lucidum na B i T ćelije
Apstrakt
Ganoderma lucidum (Leyss.: Fr.) Karst is one of the most often used mushrooms in traditional medicine of Far Eastern people. Because of its bitter taste and wooden build it is not suitable for nutrition, but the bioactive substances extracted from this mushroom possess very important medicinal characteristics. The aim of this experiment was to investigate the effects of different concentrations of isolated Ganoderma lucidum GL-I extract on the growth of JY (B) and Jurkat (T) cells. Obtained extracts were added to the cells in concentrations 1 mg/ml, 100 ng/ml, 10 ng/ml, 1 ng/ml and 100 pg/ml. JY and Jurkat cells were exposed to the action of bioactive compounds, b-glucans, during the incubation period of 72 h, at 37°C, in the atmosphere with 5% CO2 and their number was counted. Among all tested concentrations of extract the most important influence showed concentration of 1 mg/ml, which reduced the number of B cells by 61.46%, while in the case of T cells their number was reduced by 57....14%.
Ganoderma lucidum (Leyss.: Fr.) Karst je jedna od najčešće korišćenih gljiva u tradicionalnoj medicini naroda Dalekog istoka. Gorkog je ukusa i drvenaste građe, pa nije pogodna za ishranu, ali bioaktivne komponente ekstrakovane iz ove gljive pokazuju vrlo važne medicinske karakteristike. Cilj ovog rada bio je ispitivanje dejstva različitih koncentracija izdvojenog ekstrakta gljive Ganoderma lucidum GL-I na rast JY (B) i Jurkat (T) ćelija. Izdvojeni ekstrakt je dodat ćelijama u koncentracijama 1 mg/ml, 100 ng/ml, 10 ng/ml, 1 ng/ml i 100 pg/ml. JY i Jurkat ćelije su inkubirane 72 h na 37°C u atmosferi sa 5% SO2 u prisustvu bioaktivnih komponenata, polisaharida, b-glukana, a zatim je utvrđen njihov broj. Od svih primenjenih koncentracija ekstrakta najveći uticaj je pokazala koncentracija 1 mg/ml, koja je u slučaju dejstva na B ćelije dovela do smanjenja njihovog broja za 61.46%, a u slučaju dejstva na T ćelije broj je smanjen za 57.14%.
Ključne reči:
b-glucans / bioactive polysaccharides / Ganoderma lucidum / Jurkatcells / JY cellsIzvor:
Zbornik Matice srpske za prirodne nauke, 2009, 116, 217-223Izdavač:
- Matica srpska, Novi Sad
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Klaus, Anita AU - Kozarski, Maja AU - Nikšić, Miomir PY - 2009 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/2031 AB - Ganoderma lucidum (Leyss.: Fr.) Karst is one of the most often used mushrooms in traditional medicine of Far Eastern people. Because of its bitter taste and wooden build it is not suitable for nutrition, but the bioactive substances extracted from this mushroom possess very important medicinal characteristics. The aim of this experiment was to investigate the effects of different concentrations of isolated Ganoderma lucidum GL-I extract on the growth of JY (B) and Jurkat (T) cells. Obtained extracts were added to the cells in concentrations 1 mg/ml, 100 ng/ml, 10 ng/ml, 1 ng/ml and 100 pg/ml. JY and Jurkat cells were exposed to the action of bioactive compounds, b-glucans, during the incubation period of 72 h, at 37°C, in the atmosphere with 5% CO2 and their number was counted. Among all tested concentrations of extract the most important influence showed concentration of 1 mg/ml, which reduced the number of B cells by 61.46%, while in the case of T cells their number was reduced by 57.14%. AB - Ganoderma lucidum (Leyss.: Fr.) Karst je jedna od najčešće korišćenih gljiva u tradicionalnoj medicini naroda Dalekog istoka. Gorkog je ukusa i drvenaste građe, pa nije pogodna za ishranu, ali bioaktivne komponente ekstrakovane iz ove gljive pokazuju vrlo važne medicinske karakteristike. Cilj ovog rada bio je ispitivanje dejstva različitih koncentracija izdvojenog ekstrakta gljive Ganoderma lucidum GL-I na rast JY (B) i Jurkat (T) ćelija. Izdvojeni ekstrakt je dodat ćelijama u koncentracijama 1 mg/ml, 100 ng/ml, 10 ng/ml, 1 ng/ml i 100 pg/ml. JY i Jurkat ćelije su inkubirane 72 h na 37°C u atmosferi sa 5% SO2 u prisustvu bioaktivnih komponenata, polisaharida, b-glukana, a zatim je utvrđen njihov broj. Od svih primenjenih koncentracija ekstrakta najveći uticaj je pokazala koncentracija 1 mg/ml, koja je u slučaju dejstva na B ćelije dovela do smanjenja njihovog broja za 61.46%, a u slučaju dejstva na T ćelije broj je smanjen za 57.14%. PB - Matica srpska, Novi Sad T2 - Zbornik Matice srpske za prirodne nauke T1 - Influence of bioactive compounds extracted from mushroom Ganoderma lucidum on B and T cells T1 - Uticaj bioaktivnih komponenata ekstrakovanih iz gljive Ganoderma lucidum na B i T ćelije EP - 223 IS - 116 SP - 217 UR - https://hdl.handle.net/21.15107/rcub_agrospace_2031 ER -
@article{ author = "Klaus, Anita and Kozarski, Maja and Nikšić, Miomir", year = "2009", abstract = "Ganoderma lucidum (Leyss.: Fr.) Karst is one of the most often used mushrooms in traditional medicine of Far Eastern people. Because of its bitter taste and wooden build it is not suitable for nutrition, but the bioactive substances extracted from this mushroom possess very important medicinal characteristics. The aim of this experiment was to investigate the effects of different concentrations of isolated Ganoderma lucidum GL-I extract on the growth of JY (B) and Jurkat (T) cells. Obtained extracts were added to the cells in concentrations 1 mg/ml, 100 ng/ml, 10 ng/ml, 1 ng/ml and 100 pg/ml. JY and Jurkat cells were exposed to the action of bioactive compounds, b-glucans, during the incubation period of 72 h, at 37°C, in the atmosphere with 5% CO2 and their number was counted. Among all tested concentrations of extract the most important influence showed concentration of 1 mg/ml, which reduced the number of B cells by 61.46%, while in the case of T cells their number was reduced by 57.14%., Ganoderma lucidum (Leyss.: Fr.) Karst je jedna od najčešće korišćenih gljiva u tradicionalnoj medicini naroda Dalekog istoka. Gorkog je ukusa i drvenaste građe, pa nije pogodna za ishranu, ali bioaktivne komponente ekstrakovane iz ove gljive pokazuju vrlo važne medicinske karakteristike. Cilj ovog rada bio je ispitivanje dejstva različitih koncentracija izdvojenog ekstrakta gljive Ganoderma lucidum GL-I na rast JY (B) i Jurkat (T) ćelija. Izdvojeni ekstrakt je dodat ćelijama u koncentracijama 1 mg/ml, 100 ng/ml, 10 ng/ml, 1 ng/ml i 100 pg/ml. JY i Jurkat ćelije su inkubirane 72 h na 37°C u atmosferi sa 5% SO2 u prisustvu bioaktivnih komponenata, polisaharida, b-glukana, a zatim je utvrđen njihov broj. Od svih primenjenih koncentracija ekstrakta najveći uticaj je pokazala koncentracija 1 mg/ml, koja je u slučaju dejstva na B ćelije dovela do smanjenja njihovog broja za 61.46%, a u slučaju dejstva na T ćelije broj je smanjen za 57.14%.", publisher = "Matica srpska, Novi Sad", journal = "Zbornik Matice srpske za prirodne nauke", title = "Influence of bioactive compounds extracted from mushroom Ganoderma lucidum on B and T cells, Uticaj bioaktivnih komponenata ekstrakovanih iz gljive Ganoderma lucidum na B i T ćelije", pages = "223-217", number = "116", url = "https://hdl.handle.net/21.15107/rcub_agrospace_2031" }
Klaus, A., Kozarski, M.,& Nikšić, M.. (2009). Influence of bioactive compounds extracted from mushroom Ganoderma lucidum on B and T cells. in Zbornik Matice srpske za prirodne nauke Matica srpska, Novi Sad.(116), 217-223. https://hdl.handle.net/21.15107/rcub_agrospace_2031
Klaus A, Kozarski M, Nikšić M. Influence of bioactive compounds extracted from mushroom Ganoderma lucidum on B and T cells. in Zbornik Matice srpske za prirodne nauke. 2009;(116):217-223. https://hdl.handle.net/21.15107/rcub_agrospace_2031 .
Klaus, Anita, Kozarski, Maja, Nikšić, Miomir, "Influence of bioactive compounds extracted from mushroom Ganoderma lucidum on B and T cells" in Zbornik Matice srpske za prirodne nauke, no. 116 (2009):217-223, https://hdl.handle.net/21.15107/rcub_agrospace_2031 .