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Utvrđivanje rezistentnosti Myzus persicae (Sulzer) molekularnim metodama

dc.creatorVučetić, Andja
dc.creatorPetrović-Obradović, Olivera
dc.creatorMargaritopoulos, J.
dc.creatorSkouras, P.
dc.date.accessioned2020-12-17T19:20:54Z
dc.date.available2020-12-17T19:20:54Z
dc.date.issued2008
dc.identifier.issn0354-4664
dc.identifier.urihttp://aspace.agrif.bg.ac.rs/handle/123456789/1831
dc.description.abstractIn two years of investigating resistance of the peach-potato aphid Myzus persicae (Sulzer) by molecular methods, several types of resistance were established in the majority of individuals from peach and tobacco in Serbia and Montenegro. Most of the tested individuals had the FE4 gene, which encodes production of FE4 esterase. The gene responsible for kdr (knock-down resistance) was found in the majority of individuals, but in the heterozygous state, while resistance based on formation of modified acetlycholinesterase (MACE) was least represented. Also, tests showed aphids from tobacco to be more sensitive to insecticide action than aphids from peach. Three tests were used in these investiga­tions, e.g., the PCR - esterase, PCR - kdr, and RFLP - PCR tests, each for a single type of resistance.en
dc.description.abstractU dvogodišnjim istraživanjima rezistentnosti zelene breskvine vaši, Myzus persicae (Sulzer) molekularnim metodama, ustanovljeno je da je kod većine testiranih jedinki sa breskve i duvana u Srbiji i Crnoj Gori, utvrđen neki od tipova rezistentnosti. Većina testiranih jedinki imala je FE4 gen koji kodira produkciju FE4 karboksilesteraze. Gen odgovoran za kdr (knock-down rezistentnost) nađen je kod većine jedinki, ali u heterozigotnom stanju dok je rezistentnost koja se zasniva na stvaranju modifikovane acetilholinesteraze (MACE) najmanje zastupljena. Takođe, testovi su pokazali da su vaši sa duvana osetljivije na dejstvo insekticida od vašiju sa breskve. U ovim istraživanjima korišćena su tri testa: PCR-esteraza test, PCR-kdr i RFLP-PCR, svaki za po jedan tip rezistentnosti.sr
dc.publisherSrpsko biološko društvo, Beograd, i dr.
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/143006/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceArchives of Biological Sciences
dc.subjectMyzus persicae persicaeen
dc.subjectM. persicae nicotianaeen
dc.subjectresistanceen
dc.subjectesteraseen
dc.subjectkdren
dc.subjectMACEen
dc.subjectSerbiaen
dc.subjectMontenegroen
dc.titleEstablishing the resistance of Myzus persicae (Sulzer) by molecular methodsen
dc.titleUtvrđivanje rezistentnosti Myzus persicae (Sulzer) molekularnim metodamasr
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage499
dc.citation.issue3
dc.citation.other60(3): 493-499
dc.citation.spage493
dc.citation.volume60
dc.identifier.doi10.2298/ABS0803493V
dc.identifier.fulltexthttp://aspace.agrif.bg.ac.rs/bitstream/id/718/1828.pdf
dc.identifier.rcubconv_4408
dc.identifier.scopus2-s2.0-53549093031
dc.identifier.wos000258524700018
dc.type.versionpublishedVersion


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