Increased Resistance of Listeria monocytogenes and Escherichia coli O157:H7 after Exposure to Repetitive Cycles of Mild Bactericidal Treatments
Апстракт
Mild bactericidal technologies are becoming increasingly important in modem food industry. While maintaining nutritional and sensorial attributes of fresh foods these technologies generally deliver microbiologically perishable food products. Currently little information exists on possible increase in the resistance of pathogens after exposure to repetitive cycles of these bactericidal treatments. Multi-strain cocktails of two of the most important foodborne pathogens, Listeria monocytogenes and attenuated Escherichia coli O157:H7, were exposed to 20 consecutive cycles of mild inactivation by three different techniques. Used techniques comprised inactivation with lactic acid, chlorine dioxide and intense light pulses. Results showed that development of resistant mutant and/or selection of resistant cells was both species and technique dependent. While repetitive cycles of chlorine dioxide treatment did not result in increased resistance with any of three tested pathogens, lactic acid tr...eatment showed potential to create or pull out resistant cells of L. monocytogenes. Effect was determined as decreased level of reduction in bacterial counts. Even greater effect was observed for intense light pulses which showed clear trend in increased overall resistance of both L. monocytogenes and E. coli O157:H7 strains. Trend observations were confirmed through linear regression analysis. Current findings indicate ability of foodborne pathogens to adapt to mild bactericidal treatments creating new challenges in risk assessment and more specifically in hazard analysis.
Кључне речи:
Mild bactericidal treatments / increased resistance / L. monocytogenes / E. coli O157:H7Извор:
Proceedings of the 2008 Joint Central European Congress, Vol 2, 2008, 295-300Издавач:
- Croatian Chamber Economy, Zagreb
Институција/група
Poljoprivredni fakultetTY - CONF AU - Rajković, Andreja AU - Medić, Helga AU - Šmigić, Nada AU - Devlieghere, Frank PY - 2008 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/1818 AB - Mild bactericidal technologies are becoming increasingly important in modem food industry. While maintaining nutritional and sensorial attributes of fresh foods these technologies generally deliver microbiologically perishable food products. Currently little information exists on possible increase in the resistance of pathogens after exposure to repetitive cycles of these bactericidal treatments. Multi-strain cocktails of two of the most important foodborne pathogens, Listeria monocytogenes and attenuated Escherichia coli O157:H7, were exposed to 20 consecutive cycles of mild inactivation by three different techniques. Used techniques comprised inactivation with lactic acid, chlorine dioxide and intense light pulses. Results showed that development of resistant mutant and/or selection of resistant cells was both species and technique dependent. While repetitive cycles of chlorine dioxide treatment did not result in increased resistance with any of three tested pathogens, lactic acid treatment showed potential to create or pull out resistant cells of L. monocytogenes. Effect was determined as decreased level of reduction in bacterial counts. Even greater effect was observed for intense light pulses which showed clear trend in increased overall resistance of both L. monocytogenes and E. coli O157:H7 strains. Trend observations were confirmed through linear regression analysis. Current findings indicate ability of foodborne pathogens to adapt to mild bactericidal treatments creating new challenges in risk assessment and more specifically in hazard analysis. PB - Croatian Chamber Economy, Zagreb C3 - Proceedings of the 2008 Joint Central European Congress, Vol 2 T1 - Increased Resistance of Listeria monocytogenes and Escherichia coli O157:H7 after Exposure to Repetitive Cycles of Mild Bactericidal Treatments EP - 300 SP - 295 UR - https://hdl.handle.net/21.15107/rcub_agrospace_1818 ER -
@conference{ author = "Rajković, Andreja and Medić, Helga and Šmigić, Nada and Devlieghere, Frank", year = "2008", abstract = "Mild bactericidal technologies are becoming increasingly important in modem food industry. While maintaining nutritional and sensorial attributes of fresh foods these technologies generally deliver microbiologically perishable food products. Currently little information exists on possible increase in the resistance of pathogens after exposure to repetitive cycles of these bactericidal treatments. Multi-strain cocktails of two of the most important foodborne pathogens, Listeria monocytogenes and attenuated Escherichia coli O157:H7, were exposed to 20 consecutive cycles of mild inactivation by three different techniques. Used techniques comprised inactivation with lactic acid, chlorine dioxide and intense light pulses. Results showed that development of resistant mutant and/or selection of resistant cells was both species and technique dependent. While repetitive cycles of chlorine dioxide treatment did not result in increased resistance with any of three tested pathogens, lactic acid treatment showed potential to create or pull out resistant cells of L. monocytogenes. Effect was determined as decreased level of reduction in bacterial counts. Even greater effect was observed for intense light pulses which showed clear trend in increased overall resistance of both L. monocytogenes and E. coli O157:H7 strains. Trend observations were confirmed through linear regression analysis. Current findings indicate ability of foodborne pathogens to adapt to mild bactericidal treatments creating new challenges in risk assessment and more specifically in hazard analysis.", publisher = "Croatian Chamber Economy, Zagreb", journal = "Proceedings of the 2008 Joint Central European Congress, Vol 2", title = "Increased Resistance of Listeria monocytogenes and Escherichia coli O157:H7 after Exposure to Repetitive Cycles of Mild Bactericidal Treatments", pages = "300-295", url = "https://hdl.handle.net/21.15107/rcub_agrospace_1818" }
Rajković, A., Medić, H., Šmigić, N.,& Devlieghere, F.. (2008). Increased Resistance of Listeria monocytogenes and Escherichia coli O157:H7 after Exposure to Repetitive Cycles of Mild Bactericidal Treatments. in Proceedings of the 2008 Joint Central European Congress, Vol 2 Croatian Chamber Economy, Zagreb., 295-300. https://hdl.handle.net/21.15107/rcub_agrospace_1818
Rajković A, Medić H, Šmigić N, Devlieghere F. Increased Resistance of Listeria monocytogenes and Escherichia coli O157:H7 after Exposure to Repetitive Cycles of Mild Bactericidal Treatments. in Proceedings of the 2008 Joint Central European Congress, Vol 2. 2008;:295-300. https://hdl.handle.net/21.15107/rcub_agrospace_1818 .
Rajković, Andreja, Medić, Helga, Šmigić, Nada, Devlieghere, Frank, "Increased Resistance of Listeria monocytogenes and Escherichia coli O157:H7 after Exposure to Repetitive Cycles of Mild Bactericidal Treatments" in Proceedings of the 2008 Joint Central European Congress, Vol 2 (2008):295-300, https://hdl.handle.net/21.15107/rcub_agrospace_1818 .